Abstract:Chronic diseases and illnesses associated with non-specific symptoms are on the rise. In addition to chronic stress in social and work environments, physical and chemical exposures at home, at work, and during leisure activities are causal or contributing environmental stressors that deserve attention by the general practitioner as well as by all other members of the health care community. It seems necessary now to take "new exposures" like electromagnetic fields (EMF) into account. Physicians are increasingly… Show more
“…Prolonged exposure to low‐frequency PEMF is considered a carcinogen based on some epidemiological studies [Wertheimer and Leeper, ; Messam et al, ; Wang et al, ; Sermage‐Faure et al, ]. Technological advancement and continuous exposure of humans to the miscellaneous sources of these fields have heightened concern that the integrity and health of RPE cells are in danger from these fields [Belyaev et al, ]. Further investigations with respect to cellular and molecular changes of RPE cells exposed to different sets of PEMFs should be performed.…”
A great deal of evidence has confirmed that electromagnetic fields (EMFs) can affect the central nervous system. In this study, cultured neonatal human retinal pigment epithelial (hRPE) cells were exposed to pulsed EMF of 1 mT intensity and 50 Hz frequency 8 h daily for 3 days. In addition to cell proliferation and cell death assays, immunocytochemistry for RPE65, PAX6, nestin, and cytokeratin 8/18 proteins were performed. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for NES, PAX6, RPE65, and ACTA2 gene expression. Exposed hRPE cells did not demonstrate significant change in terms of cytomorphology, cell proliferation, or cell death. Protein expression of PAX6 was decreased in treated cells compared to controls and remained unchanged for RPE65, cytokeratin 8/18, and nestin. Gene expressions of NES, RPE65, and PAX6 were decreased in treated cells as compared to controls. Gene expression of ACTA2 did not significantly change. In conclusion, viability of cultivated neonatal hRPE cells did not change after short exposure to a safe dose of pulsed EMF albeit that both gene and protein expressions of retinal progenitor cell markers were reduced. Whether longer exposure durations that are being constantly produced by widely-used electronic devices may induce significant changes in these cells, needs further investigation. Bioelectromagnetics. 39:585-594, 2018.
“…Prolonged exposure to low‐frequency PEMF is considered a carcinogen based on some epidemiological studies [Wertheimer and Leeper, ; Messam et al, ; Wang et al, ; Sermage‐Faure et al, ]. Technological advancement and continuous exposure of humans to the miscellaneous sources of these fields have heightened concern that the integrity and health of RPE cells are in danger from these fields [Belyaev et al, ]. Further investigations with respect to cellular and molecular changes of RPE cells exposed to different sets of PEMFs should be performed.…”
A great deal of evidence has confirmed that electromagnetic fields (EMFs) can affect the central nervous system. In this study, cultured neonatal human retinal pigment epithelial (hRPE) cells were exposed to pulsed EMF of 1 mT intensity and 50 Hz frequency 8 h daily for 3 days. In addition to cell proliferation and cell death assays, immunocytochemistry for RPE65, PAX6, nestin, and cytokeratin 8/18 proteins were performed. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for NES, PAX6, RPE65, and ACTA2 gene expression. Exposed hRPE cells did not demonstrate significant change in terms of cytomorphology, cell proliferation, or cell death. Protein expression of PAX6 was decreased in treated cells compared to controls and remained unchanged for RPE65, cytokeratin 8/18, and nestin. Gene expressions of NES, RPE65, and PAX6 were decreased in treated cells as compared to controls. Gene expression of ACTA2 did not significantly change. In conclusion, viability of cultivated neonatal hRPE cells did not change after short exposure to a safe dose of pulsed EMF albeit that both gene and protein expressions of retinal progenitor cell markers were reduced. Whether longer exposure durations that are being constantly produced by widely-used electronic devices may induce significant changes in these cells, needs further investigation. Bioelectromagnetics. 39:585-594, 2018.
“…The first evidence of possible health risks connected to exposures to EMFs dates back to 1979, when reports on the possible pathological effects of the prolonged proximity to certain configurations of power lines [Wertheimer and Leeper, ] or exposure to radar‐emitted radiofrequencies [Robinette et al, ] were first published. As evidence started to accumulate, the idea, albeit controversial, that chronic exposure to EMFs could pose a threat to human health was developed [Budinger, ; Wertheimer and Leeper, ; Kavet and Banks, ; Brown and Chattopadhyay, ; Kavet, ; Tenforde, ; Habash et al, ; Bellieni et al, ; Rodríguez‐De la Fuente et al, ; Carpenter, ; Monazzam et al, ; Belyaev et al, ; Pall, , ; Lasalvia et al, ] to the point that 220 scientists from different countries in the world signed an international appeal to the United Nations and World Health Organization (WHO) in 2016 highlighting the need to raise awareness in the general public and in health operators about the risks associated with chronic exposure to EMFs [Hardell, ]. This necessity has also been acknowledged by WHO and several governments [Belyaev et al, ].…”
Section: Electromagnetic Field Effects On Cells and Tissuesmentioning
“…Firstly, it should be noticed that the need of readdressing the subject comes not only from scientific purism, but from two very sensitive issues for the general public: (i) the possibility of ELF-MF being carcinogenic [IARC, 2002;Villeneuve et al, 2002;Kabuto et al, 2006;Sch€ uz, 2011;Belyaev et al, 2016;Carlberg et al, 2017] and (ii) the possibility of using ELF-MF as therapeutic agents against cancer and other diseases [Shupak et al, 2003;Funk et al, 2009;Novikov et al, 2009;Hu et al, 2010;Tatarov et al, 2011;Nie et al, 2013;Buckner et al, 2015]. Indeed, even though the controversy has not evolved much in recent decades, the relevance of magnetobiology has risen in the eyes of scientists, the general public, governments, and international organizations, which have repeatedly pointed out inconsistencies among experimental results [IARC, 2002b;WHO, 2007;ICNIRP, 2010].…”
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