Euploidization of human hepatocytes from donors of different ages and both sexes compared with those from cases of werner's syndrome and progeria

Gahan, P. B.; Middleton, J.

doi:10.1016/0531-5565(84)90044-5

Cited by 14 publications

(11 citation statements)

References 6 publications

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“…Unfortunately, small sample sizes and the qualitative nature of many of these studies preclude any meaningful correlations with functional analyses (13). While nuclear ploidy increases in rodent livers, the data from human tissue are conflicting (14)(15)(16)(17). A recent study in humans reported that the rate of hepatocyte polyploidization is slow during the first five decades of life, but that it increases substantially, with subjects between 86 and 92 years of age exhibiting 27% polyploid nuclei (18).…”

Section: Liver Morphologymentioning

confidence: 98%

Aging and the Liver: An Update

Schmucker

1998

The Journals of Gerontology Series A: Biological Sciences and M

155

103

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Section: Liver Morphologymentioning

confidence: 98%

Aging and the Liver: An Update

Schmucker

1998

The Journals of Gerontology Series A: Biological Sciences and M

155

103

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“…For example, Shima (1978) reported a 90% drop in the number of diploid hepatocytes accompanied by increases in several polyploid classes in mice between 1 and 28 months of age. However, Gahan and Middleton (1984) reported that ANIMAL AGE (Months) Fig. 6.…”

Section: Hepatocyte and Nuclear Volumesmentioning

confidence: 99%

Hepatocyte fine structure during maturation and senescence

Schmucker

1990

J. Elec. Microsc. Tech.

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Aging is accompanied by a myriad of changes in cell structure, function, and composition. The fact that much of the information concerning age-related alterations in cellular morphology is qualitative precludes meaningful correlations with biochemical changes in order to enhance data interpretation. The mammalian liver has been subjected to both qualitative and quantitative evaluations of hepatocyte structure as a function of aging, i.e., development, maturation, and senescence. Although these data are characterized by considerable variability and, in some instances, blatant contradictions, there exists sufficient agreement in several parameters to permit a consensus in the inbred rat model. Certainly the volume of individual hepatocytes increases with age, and many of the organelle compartments reflect this change. While old rats exhibit a high incidence of polyploidy, there is no definitive evidence to demonstrate a concomitant increase in the binuclear hepatocyte index. Several specific hepatocellular organelles undergo changes in their relative volume or surface area that appear to correlate with functional alterations. The volume density of the lysosomal compartment enlarges significantly during senescence and is accompanied by increased activities of several constituent hydrolases. The hepatic concentration of smooth-surfaced endoplasmic reticulum declines markedly with aging, as does the yield of liver microsomes and the activities of several microsomal enzymes, e.g., mono-oxygenases and glucose-6-phosphatase. However, the responses of the majority of hepatocyte organelles to aging is varied and inconsistent based on the limited data currently available.

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“…In human liver, Swartz (1956) and Gahan and Middleton (1984) are in basic agreement that one class of diploid hepatocytes is present up to 6 years of age, that a tetraploid class appears between 6 and 10 years of age, and that an octaploid class appears at 20 years of age. Since these studies have been mainly concerned with the time of origin of ploidy classes, only a small number of older individuals was examined.…”

Section: Introductionmentioning

confidence: 82%

“…Swartz (1956) studied 4 individuals 40 to 90 years of age. Gahan and Middleton (1984) studied 4 individuals 56 to 80 years of age. By examining a larger number of older individuals in the present study, we undertook to answer the following questions: (1) Does the process of polyploidization continue throughout the human lifespan as it does in mice, or does it reach a plateau at which point polyploid cell formation ceases?…”

Section: Introductionmentioning

confidence: 99%

Changes in Polyploidy During Aging in Human Liver

Enesco¹,

Laskey²,

Fisman³

1986

Can. J. Aging

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The level of liver polyploidy was compared in three groups of humans: a young group aged 20–46, an older group aged 62–68 and the oldest group aged 70–79. The most important trend showed by the data was that there is a depletion of 2N and 4N cells, accompanied by an increase in 8N and 16N cells as a function of advancing age. There was a high degree of variability in the percentage of cells in each ploidy class from one individual to the next for all age groups. For this reason, polyploidy appears to be a less effective biomarker of aging in humans than it is in experimental animals.

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Euploidization of human hepatocytes from donors of different ages and both sexes compared with those from cases of werner's syndrome and progeria

Cited by 14 publications

References 6 publications

Aging and the Liver: An Update

Aging and the Liver: An Update

Hepatocyte fine structure during maturation and senescence

Changes in Polyploidy During Aging in Human Liver

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