Euphorbia kansui roots induced-diarrhea in mice correlates with inflammatory response

Chai, Yu-Shuang; Hu, Jun; Wang, Xiukun; Wang, Yugang; Xiao, Xinyue; Cheng, Xian‐Long; Hua, Lei; Fan, Lei; Xing, Dongming; Du, Lijun

doi:10.3724/sp.j.1009.2013.00231

Cited by 13 publications

(4 citation statements)

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“…It can cause acute toxicity such as skin and gastrointestinal irritation, nervous system toxicity such as dyskinesia, ataxia, muscle spasm and mental malaise. 8,9 It can also cause long-term toxicity such as hepatic injury and promotion of tumors, 10,11 which have seriously restricted its clinical application. A unique processing method, which is called Paozhi, is a common traditional approach that usually occurs before some herbs are prescribed.…”

Section: Introductionmentioning

confidence: 99%

“…However, raw Kansui has a degree of toxicity. It can cause acute toxicity such as skin and gastrointestinal irritation, nervous system toxicity such as dyskinesia, ataxia, muscle spasm and mental malaise 8,9 . It can also cause long‐term toxicity such as hepatic injury and promotion of tumors, 10,11 which have seriously restricted its clinical application.…”

Section: Introductionmentioning

confidence: 99%

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Detoxification mechanism of vinegar‐processed Kansui revealed by systematic phytochemical analysis using ultrahigh‐performance liquid chromatography diode array detection tandem mass spectrometry, ultrahigh‐performance liquid chromatography high‐resolution mass spectrometry and in silico drug target identification

Lian

Lv³

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale The dried roots of Euphorbia kansui L., known as Kansui, are used to treat ascites and edema in traditional Chinese medicine. However, the toxicity of this herb has seriously restricted its clinical application. A unique vinegar‐processing method has been used to reduce its toxicity since the time of ancient China. However, the detoxification mechanism underlying such vinegar processing has not been fully revealed. To find the answer, the process‐induced changes in components should be carefully investigated. Methods We performed a systematic analysis of chemical components in raw and vinegar‐processed Kansui using ultrahigh‐performance liquid chromatography (UHPLC) diode array detection tandem mass spectrometry and UHPLC high‐resolution mass spectrometry. Thirty‐one chemical components in raw and vinegar‐processed Kansui were found, the chemical structures of 28 components among them were proposed and the process‐induced changes in components were then investigated. Results A comprehensive conclusion about the process‐induced chemical change was drawn. It was found that jatrophane‐type diterpenoids decreased markedly after vinegar processing, while ingenane‐type diterpenoids were retained during vinegar processing. In silico drug target identification gave hints that jatrophane‐type diterpenoids, which decreased markedly during vinegar processing, may have more intense toxicity involving cholinesterase and mitogen‐activated protein kinases, while ingenane‐type diterpenoids, which were retained during vinegar processing, may have a more intense therapeutic effect involving carbonic anhydrase. Conclusions The possible detoxification mechanism of vinegar‐processed Kansui is presented. The research has significance for the therapeutic/toxic chemical basis of Kansui. Also, it has significance for drug discovery from terpenoids within the herb.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detoxification mechanism of vinegar‐processed Kansui revealed by systematic phytochemical analysis using ultrahigh‐performance liquid chromatography diode array detection tandem mass spectrometry, ultrahigh‐performance liquid chromatography high‐resolution mass spectrometry and in silico drug target identification

Lian

Lv³

et al. 2022

Rapid Comm Mass Spectrometry

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“…Kansuinine A (KA; Figure 1 A) is extracted from Euphorbia kansui L., a well-known medicinal plant in China. Euphorbia kansui extract acts on the intestinal smooth muscles to stimulate the intestines, resulting in the propulsion of feces [ 16 ]. Furthermore, 3- O -(2,3-dimethylbutanoyl)-13- O -decanoylingenol, purified from E. kansui extract, displays therapeutic potential against allergic diseases, and the underlying mechanism involves the inhibition of intracellular signaling pathways to activate and release chemical mediators from mast cells [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling

Chen

Pan

Tsai

et al. 2021

IJMS

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Reactive oxygen species (ROS)-induced vascular endothelial cell apoptosis is strongly associated with atherosclerosis progression. Herein, we aimed to examine whether Kansuinine A (KA), extracted from Euphorbia kansui L., prevents atherosclerosis development in a mouse model and inhibits cell apoptosis through oxidative stress reduction. Atherosclerosis development was analyzed in apolipoprotein E-deficient (ApoE−/−) mice fed a high-fat diet (HFD) using Oil Red O staining and H&E staining. Human aortic endothelial cells (HAECs) were treated with KA, followed by hydrogen peroxide (H2O2), to investigate the KA-mediated inhibition of ROS-induced oxidative stress and cell apoptosis. Oil Red O staining and H&E staining showed that atherosclerotic lesion size was significantly smaller in the aortic arch of ApoE−/− mice in the HFD+KA group than that in the aortic arch of those in the HFD group. Further, KA (0.1–1.0 μM) blocked the H2O2-induced death of HAECs and ROS generation. The H2O2-mediated upregulation of phosphorylated IKKβ, phosphorylated IκBα, and phosphorylated NF-κB was suppressed by KA. KA also reduced the Bax/Bcl-2 ratio and cleaved caspase-3 expression, preventing H2O2-induced vascular endothelial cell apoptosis. Our results indicate that KA may protect against ROS-induced endothelial cell apoptosis and has considerable clinical potential in the prevention of atherosclerosis and cardiovascular diseases.

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“…In clinics, it is often used to treat pancreatitis and intestinal obstruction (Fan et al, 2005;Ge et al, 2009;Li et al, 2002;Ouyang et al, 2004). In recent years, its anti-inflammatory (Dutra et al, 2012a;Chai et al, 2013), anti-viral (Zheng, 2004) and anti-fertility properties (Han et al, 2011), as well as its ability to regulate the immune system, have been reported (Nunomura et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of euphol in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study

Xie

Gao

et al. 2014

Biomedical Chromatography

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Euphol is a potential pharmacologically active ingredient isolated from Euphorbia kansui. A simple, rapid, and sensitive method to determine euphol in rat plasma was developed based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the first time. The analyte and internal standard (IS), oleanic acid, were extracted from plasma with methanol and chromatographied on a C18 short column eluted with a mobile phase of methanol–water–formic acid (95:5:0.1, v/v/v). Detection was performed by positive ion atmospheric pressure chemical ionization in selective reaction monitoring mode. This method monitored the transitions m/z 409.0 →109.2 and m/z 439.4 → 203.2 for euphol and IS, respectively. The assay was linear over the concentration range 27–9000 ng/mL, with a limit of quantitation of 27 ng/mL. The accuracy was between –7.04 and 4.11%, and the precision was <10.83%. This LC-MS/MS method was successfully applied to investigate the pharmacokinetic study of euphol in rats after intravenous (6 mg/kg) and oral (48 mg/kg) administration. Results showed that the absolute bioavailability of euphol was approximately 46.01%.

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Euphorbia kansui roots induced-diarrhea in mice correlates with inflammatory response

Abstract: The results showed that the extract of E. KS acted on the intestinal smooth muscle with propulsion of feces involving the irritation of the intestines with acute inflammatory reactions.

Cited by 13 publications

References 0 publications

Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling

Quantitative determination of euphol in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study

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