Eupatilin suppresses the allergic inflammatory response in vitro and in vivo

Song, Eunhye; Chung, Kyung‐Sook; Kang, Yun‐Mi; Lee, Jong‐Hyun; An, Hyo‐Jin

doi:10.1016/j.phymed.2017.08.027

Cited by 30 publications

(25 citation statements)

References 34 publications

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“…MAPKs phosphorylation are also crucial events in the allergic inflammation response. The maximal MAPK (JNK, ERK, p38) expression is known to occur 10 ∼ 30 min after LPS stimulation (Reddy and Reddanna, 2009; Song et al, 2018). RAW264.7 cells were pretreated with ZCO for 4 h, and then stimulated with LPS for 30 min.…”

Section: Discussionmentioning

confidence: 99%

“…When stimulated, NF-κB translocates to the nucleus, and regulates the expression of various transcription factors leading to the expression of pro-inflammatory enzymes like cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS), which are responsible for the stimulation of inflammatory signaling molecules (Ahmad et al, 2017). Apart from NF-κB, MAPKs also plays an important role in the regulation of the cell growth, cell differentiation, and cellular pro-inflammatory molecules (Huang et al, 2012; Song et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai

Guo

Park

et al. 2018

Front. Pharmacol.

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Zanthoxylum coreanum Nakai is a rare shrub which grows in Korea and China. Pericarp of Z. coreanum has been used as a crude medicine, but there are few researches about the pharmacologic activities. The present study was designed to investigate the anti-allergic inflammatory activities of the essential oil from fruits of Zanthoxylum coreanum Nakai (ZCO). Our findings showed that ZCO inhibited both the IgE-antigen complex or PMA/A23187-induced β-hexosaminidase release and IL-4 production dose-dependently in RBL-2H3 mast cells, and confirmed that ZCO at the tested concentrations did not show cytotoxicity to RBL-2H3 cells by MTS assay. Additionally, we found that ZCO showed the significant inhibition on LPS-induced overproduction of TNF-α, IL-6 and NO. Consistently, the protein levels of iNOS and COX-2 were also remarkably decreased by ZCO treatment. Herein, Our mechanistic studies revealed that ZCO significantly suppressed the activation of transcription factor NF-κB in PMA-activated 293T cells, and further inhibited NF-κB p65 translocation into the nucleus in LPS-stimulated RAW264.7 cells. Further investigation identified that ZCO down-regulated LPS-induced phosphorylation of MAPK (JNK, ERK, and p38) signal pathway. For incremental research, we established an DNCB-induced atopic dermatitis model in BALB/c mice, and found that ZCO remarkably inhibited DNCB-induced ear swelling and AD-like symptoms. Based on these findings, ZCO is suggested to have a therapeutic potential for the allergic inflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai

Guo

Park

et al. 2018

Front. Pharmacol.

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“…Eupatilin can suppress renal cancer growth by inhibiting the expression of miR-21, which targets yes-associated protein 1 [43]. Regarding its anti-inflammatory effect, eupatilin downregulated the expression of pro-inflammatory cytokines, such as IL-1β, TNF-α, and IL-6, in an anaphylactic shock model by regulating NF-κB and MAPK signaling [44].…”

Section: Eupatilinmentioning

confidence: 99%

Therapeutic Single Compounds for Osteoarthritis Treatment

et al. 2021

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Osteoarthritis (OA) is an age-related degenerative disease for which an effective disease-modifying therapy is not available. Natural compounds derived from plants have been traditionally used in the clinic to treat OA. Over the years, many studies have explored the treatment of OA using natural extracts. Although various active natural extracts with broad application prospects have been discovered, single compounds are more important for clinical trials than total natural extracts. Moreover, although natural extracts exhibit minimal safety issues, the cytotoxicity and function of all single compounds in a total extract remain unclear. Therefore, understanding single compounds with the ability to inhibit catabolic factor expression is essential for developing therapeutic agents for OA. This review describes effective single compounds recently obtained from natural extracts and the possibility of developing therapeutic agents against OA using these compounds.

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“…Eupatilin (5,7-dihydroxy-3,4,6-trimethoxyflavone) is a lipophilic polymethoxylated flavone isolated from Artemisia asiatica. Eupatilin has been reported to have antioxidative, antiinflammatory, and anti-allergic effects, although the underlying mechanisms are unclear [11][12][13][14]. Moreover, eupatilin has been reported to have an anti-inflammatory effect on the TGF-β pathway [9].…”

Section: Introductionmentioning

confidence: 99%

Antifibrotic effects of eupatilin on TGF-β1-treated human vocal fold fibroblasts

et al. 2021

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Vocal fold scarring is a major cause of dysphonia. Vocal fold fibroblasts (VFFs) and the TGF-β signaling pathway play important roles in scar formation. Eupatilin, a chromone derivative of the Artemisia species, is a traditional folk remedy for wound healing. However, until recently, few studies investigated the therapeutic effects of eupatilin. We investigated the antifibrogenic effects of eupatilin on TGF-β1-treated human vocal fold fibroblasts (hVFFs). The optimal concentration of eupatilin was determined by a cell viability assay. Western blotting was used to measure the expression of alpha-smooth muscle actin during myofibroblast differentiation, fibronectin (FN), collagen type I (Col I), and collagen type III (Col III) extracellular matrix proteins, and Smad2, Smad3, and p38 in the fibrotic pathway. Measurements were made before and after eupatilin treatment. Eupatilin at 100 nM was shown to be safe for use in hVFFs. TGF-β1 induced hVFFs to proliferate and differentiate into myofibroblasts and increased Col III and FN synthesis in a time- and dose-dependent manner. Eupatilin suppressed TGF-β1-induced hVFF proliferation and differentiation into myofibroblasts through the Smad and p38 signaling pathways. Furthermore, eupatilin inhibited TGF-β1-induced FN, Col I, and Col III synthesis in hVFFs. Our in vitro findings show that eupatilin effectively suppressed TGF-β1-induced fibrotic changes in hVFFs via the Smad and p38 signaling pathways. Thus, eupatilin may be considered a novel therapeutic agent for the treatment of vocal fold fibrosis.

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Eupatilin suppresses the allergic inflammatory response in vitro and in vivo

Cited by 30 publications

References 34 publications

Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai

Anti-allergic Inflammatory Effects of the Essential Oil From Fruits of Zanthoxylum coreanum Nakai

Therapeutic Single Compounds for Osteoarthritis Treatment

Antifibrotic effects of eupatilin on TGF-β1-treated human vocal fold fibroblasts

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