Eupatilin Suppresses Pancreatic Cancer Cells <i>via</i> Glucose Uptake Inhibition, AMPK Activation, and Cell Cycle Arrest

PARK, TAE HYUN; KIM, HYEON SOO

doi:10.21873/anticanres.15506

Cited by 9 publications

(3 citation statements)

References 32 publications

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“…In the present study, it was found that VNN1 inhibited the phosphorylation of AMPK in PC cells. As activated AMPK can suppress the invasion and migration of PC cells ( 45 , 46 ), it was hypothesized that VNN1 may promote PC progression by inhibiting AMPK signaling pathway. Therefore, it may be noteworthy to investigate the role of VNN1 in PC progression and develop potential VNN1-targeted therapies for PC in the future.…”

Section: Discussionmentioning

confidence: 99%

VNN1 overexpression in pancreatic cancer cells inhibits paraneoplastic islet function by increasing oxidative stress and inducing β‑cell dedifferentiation

Qin

Kang

et al. 2023

Oncol Rep

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Vanin-1 (VNN1) may be a potential biomarker for the early screening of pancreatic cancer (PC)-associated diabetes (PCAD). A previous study by the authors reported that cysteamine secreted by VNN1-overexpressing PC cells induced the dysfunction of paraneoplastic insulinoma cell lines by increasing oxidative stress. In the present study, it was observed that both cysteamine and exosomes (Exos) secreted by VNN1-overexpressing PC cells aggravated the dysfunction of mouse primary islets. PC-derived VNN1 could be transported into islets through PC cell-derived Exos (PC-Exos). However, β-cell dedifferentiation, and not cysteamine-mediated oxidative stress, was responsible for the islet dysfunction induced by VNN1-containing Exos. VNN1 inhibited the phosphorylation of AMPK and GAPDH, and prevented Sirt1 activation and FoxO1 deacetylation in islets, which may be responsible for the induction of β-cell dedifferentiation induced by VNN1-overexpressing PC-Exos. Furthermore, it was demonstrated that VNN1-overexpressing PC cells further impaired the functions of paraneoplastic islets in vivo using diabetic mice with islets transplanted under the kidney capsule. On the whole, the present study demonstrates that PC cells overexpressing VNN1 exacerbate the dysfunction of paraneoplastic islets by inducing oxidative stress and β-cell dedifferentiation.

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Section: Discussionmentioning

confidence: 99%

VNN1 overexpression in pancreatic cancer cells inhibits paraneoplastic islet function by increasing oxidative stress and inducing β‑cell dedifferentiation

Qin

Kang

et al. 2023

Oncol Rep

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“…Research has shown that quercetin and quercetin 3-O-glucuronide regulate the sleep-wake cycle by activating the GABA(A) receptor and improving sleep duration and quality of insomnia mice ( Kambe et al, 2010 ; Kim et al, 2021 ). Eupatilin has been shown to inhibit pancreatic cancer cells, the growth of kidney cancer cells, and the metastasis of prostate cancer cells while improving the immune system ( Zhong et al, 2019 ; Serttas et al, 2021 ; Park and Kim, 2022 ). Our study first reports that eupatilin may improve sleep of a sleep-disturbed zebrafish, but it requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Aquilaria sinensis leaf tea affects the immune system and increases sleep in zebrafish

Tan,

Wang,

Smith

et al. 2023

Front. Pharmacol.

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The importance of adequate sleep for good health cannot be overstated. Excessive light exposure at night disrupts sleep, therefore, it is important to find more healthy drinks that can promote sleep under sleep-disturbed conditions. The present study investigated the use of A. sinensis (Lour.) Spreng leaf tea, a natural product, to reduce the adverse effects of nighttime light on sleep. Here, Aquilaria sinensis leaf tea at 1.0 and 1.5 g/L significantly increased sleep time in zebrafish larvae (5–7 dpf) with light-induced sleep disturbance. Transcriptome sequencing and qRT-PCR analysis revealed a decrease in the immune-related genes, such as nfkbiab, tnfrsf1a, nfkbiaa, il1b, traf3, and cd40 in the 1.5 g/L Aquilaria sinensis leaf tea treatment group. In addition, a gene associated with sleep, bhlhe41, showed a significant decrease. Moreover, Aquilaria sinensis leaf tea suppressed the increase in neutrophils of Tg(mpo:GFP) zebrafish under sleep-disturbed conditions, indicating its ability to improve the immune response. Widely targeted metabolic profiling of the Aquilaria sinensis tea using ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry (UPLC-ESI-MS/MS) revealed flavonoids as the predominant component. Network pharmacological and molecular docking analyses suggested that the flavonoids quercetin and eupatilin in Aquilaria sinensis leaf tea improved the sleep of zebrafish by interacting with il1b and cd40 genes under light exposure at night. Therefore, the results of the study provide evidence supporting the notion that Aquilaria sinensis leaf tea has a positive impact on sleep patterns in zebrafish subjected to disrupted sleep due to nighttime light exposure. This suggests that the utilization of Aquilaria sinensis leaf tea as a potential therapeutic intervention for sleep disturbances induced by light may yield advantageous outcomes.

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“…In addition, the experimental results showed that the two flavonoids, jaceosidin, and eupatilin, found in Artemisiae Argyi Folium samples with an average content of 0.56 and 1.15 mg/g, respectively, were not detected in Artemisiae Verlotori Folium samples. According to the literature, jaceosidin has antioxidant, anticancer, antiinflammatory, anti-allergic, and antibacterial activities [34][35][36][37][38], and eupatilin has good anti-inflammatory, antioxidant, anti-apoptotic and antitumor effects [39][40][41][42][43]. The nature of Artemisiae Verlotori Folium is milder than that of Artemisiae Argyi Folium and is likely to be related to the difference in these two components between the two species, which remains to be further studied.…”

Section: Ta B L Ementioning

confidence: 99%

Quantitative and qualitative analysis of Artemisiae Verlotori Folium and Artemisiae Argyi Folium by high‐performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Deng

et al. 2023

J of Separation Science

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Artemisiae Argyi Folium (Aiye in Chinese) has been widely used since ancient times. In the Lingnan region (Southern China), the leaf of Artemisia verlotorum Lamotte, which is named Hongjiaoai (HJA) because the roots are red (Hongjiao means red foot in Chinese), has been used as a local substitute for Artemisiae Argyi Folium. The plant has a long medicinal and edible history that can be traced to the Jin Dynasty. However, there is no systematic and reliable method to control the quality of Artemisiae Verlotori Folium. In this study, a comprehensive method involving high-performance liquid chromatography coupled with diode array detection and quadrupole-time-of-flight high-definition mass spectrometry was established to identify and quantify eight constituents (including organic acids and flavonoids) in Artemisiae Verlotori Folium and Artemisiae Argyi Folium as well as high-performance liquid chromatography fingerprints of the two varieties. Moreover, dissimilarities of chemical compositions among the two varieties were further investigated by orthogonal partial least squares discrimination analysis and cluster analysis. This research not only explored the similarities and differences between Artemisiae Verlotori Folium and Artemisiae Argyi Folium in eight components but also provided a qualitative and quantitative analytical method that quickly, accurately, and comprehensively assesses the quality of Artemisiae Verlotori Folium.Article Related Abbreviations: DAD, diode array detector; HCA, hierarchical clustering; HPLC-DAD-Q-TOF-MS, HPLC coupled with diode array detector and quadrupole-time-of-flight mass spectrometry; OPLS-DA, orthogonal partial least squares discriminant analysis; TCM, traditional Chinese medicine; VIP, variable importance in the projection.

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Eupatilin Suppresses Pancreatic Cancer Cells via Glucose Uptake Inhibition, AMPK Activation, and Cell Cycle Arrest

Cited by 9 publications

References 32 publications

VNN1 overexpression in pancreatic cancer cells inhibits paraneoplastic islet function by increasing oxidative stress and inducing β‑cell dedifferentiation

VNN1 overexpression in pancreatic cancer cells inhibits paraneoplastic islet function by increasing oxidative stress and inducing β‑cell dedifferentiation

Aquilaria sinensis leaf tea affects the immune system and increases sleep in zebrafish

Quantitative and qualitative analysis of Artemisiae Verlotori Folium and Artemisiae Argyi Folium by high‐performance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

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