Eupatilin Alleviates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting Inflammation and Oxidative Stress

Liu, Haiying; Hao, Jingwen; Wu, Chunyuan; Liu, Guosheng; Wang, Xing; Yu, Jieming; Liu, Yu; Zhao, Hongxia

doi:10.12659/msm.917406

Cited by 23 publications

(20 citation statements)

References 30 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There have been studies on the antioxidant role of eupatilin in epithelial cells of various organs [ 18 , 29 ]. As an example, there was an in vivo experiment confirming that eupatilin inhibited the production of ROS and inflammatory cytokines in acute lung injury induced by LPS [ 17 ]. As can be seen from our study results, the generation of ROS induced in bronchial epithelial cells due to FPM is inhibited by eupatilin, suggesting that eupatilin could be considered as a treatment for respiratory diseases caused by FPM.…”

Section: Discussionmentioning

confidence: 99%

“…Eupatilin (5,7-dihydroxy-3,4,6-trimethoxyflavone) is an active flavone extracted from Artemisia species and has been reported to have antioxidant, anti-inflammatory, anti-cancer, and neuroprotective effects [ 15 , 16 , 17 , 18 ]. Based on the literature, we hypothesized that eupatilin could reduce oxidative damage to respiratory cells caused by FPM.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells

Lee

Choi

et al. 2021

Toxics

View full text Add to dashboard Cite

Background: Eupatilin is an active flavon extracted from the Artemisia species and has properties such as antioxidant, anti-inflammatory, and anti-cancer. We examined the effect of eupatilin using fine particulate matter (FPM) and human bronchial epithelial cell line (BEAS-2B) to confirm the potential of eupatilin as a therapeutic agent for respiratory diseases caused by FPM. Methods: Reactive oxygen species (ROS) levels were checked by flow cytometry to identify if FPM and eupatilin affect ROS production. Western blotting was performed to identify the mechanism of action of eupatilin in FPM-exposed BEAS-2B cells. Results: When cells were exposed to FPM above 12.5 μg/mL concentration for 24 h, ROS production increased significantly compared to the control. When eupatilin was added to cells exposed to FPM, the ROS level decreased proportionally with the eupatilin dose. The phosphorylation of Akt, NF-κB p65, and p38 MAPK induced by FPM was significantly reduced by eupatilin, respectively. Conclusion: FPM cause respiratory disease by producing ROS in bronchial epithelial cells. Eupatilin has been shown to inhibit ROS production through altering signaling pathways. The ROS inhibiting property of eupatilin can be exploited in FPM induced respiratory disorders.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells

Lee

Choi

et al. 2021

Toxics

View full text Add to dashboard Cite

show abstract

“…[ 7 18 19 20 ] As we discussed, there are two pharmacologically active flavone compounds, eupatilin, and its hydroxylated form, in A. kermanensis with their wide range of biological activity. Eupatilin is known for its anticancer,[ 17 18 21 22 23 24 25 ] anti-inflammatory,[ 26 27 ] anti-oxidant,[ 28 ] neuroprotective,[ 29 ] and antiallergic[ 30 ] activities. It is speculated that eupatilin could be subjected to structural optimization to synthesize derivative analogs to reinforce its efficacy, minimize toxicity, and optimize absorption profiles, ultimately leading to potent drug candidates.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of methylated flavones from Artemisia kermanensis

2021

View full text Add to dashboard Cite

Background: Artemisia kermanensis Podl. is a green aromatic perennial shrub that belongs to the family Asteraceae and it grows widely in central deserts and south-eastern mountains of Iran such as Taftan Mountain in Sistan and Baluchestan Province. Artemisia species have been used traditionally as a remedy for various feverous diseases, including malaria, treatment of colds, infections, parasites, inflammations of the liver, as well as dyspepsia, diabetes, hypertension, and so many other conditions. Materials and Methods: Air-dried A. kermanensis extraction from all parts of the plant was done using different organic solvents. The methanolic extract was selected for isolation of flavonoids, using thin-layer chromatography. The chemical structures of the isolated compounds were determined based on analysis of mass and nuclear magnetic resonance spectra. Results: Two flavone aglycones were isolated and identified for the first time from this plant's methanolic extract, including 5,7-dihydroxy-3',4',6-trimethoxyflavone (eupatilin) and 5,7,3'-Trihydroxy-6,4',5'-trimethoxyflavone. Conclusions: Eupatilin is known for its anticancer, antioxidant, and anti-inflammatory activities. In future researches on A. kermanensis , as a rich source of these flavone compounds, it is wise to investigate for the proven eupatilin's biological activities that have been mentioned.

show abstract

“…Eupatilin (5,7-dihydroxy-3,4,6-trimethoxyflavone) is a lipophilic polymethoxylated flavone isolated from Artemisia asiatica. Eupatilin has been reported to have antioxidative, antiinflammatory, and anti-allergic effects, although the underlying mechanisms are unclear [11][12][13][14]. Moreover, eupatilin has been reported to have an anti-inflammatory effect on the TGF-β pathway [9].…”

Section: Introductionmentioning

confidence: 99%

Antifibrotic effects of eupatilin on TGF-β1-treated human vocal fold fibroblasts

et al. 2021

View full text Add to dashboard Cite

Vocal fold scarring is a major cause of dysphonia. Vocal fold fibroblasts (VFFs) and the TGF-β signaling pathway play important roles in scar formation. Eupatilin, a chromone derivative of the Artemisia species, is a traditional folk remedy for wound healing. However, until recently, few studies investigated the therapeutic effects of eupatilin. We investigated the antifibrogenic effects of eupatilin on TGF-β1-treated human vocal fold fibroblasts (hVFFs). The optimal concentration of eupatilin was determined by a cell viability assay. Western blotting was used to measure the expression of alpha-smooth muscle actin during myofibroblast differentiation, fibronectin (FN), collagen type I (Col I), and collagen type III (Col III) extracellular matrix proteins, and Smad2, Smad3, and p38 in the fibrotic pathway. Measurements were made before and after eupatilin treatment. Eupatilin at 100 nM was shown to be safe for use in hVFFs. TGF-β1 induced hVFFs to proliferate and differentiate into myofibroblasts and increased Col III and FN synthesis in a time- and dose-dependent manner. Eupatilin suppressed TGF-β1-induced hVFF proliferation and differentiation into myofibroblasts through the Smad and p38 signaling pathways. Furthermore, eupatilin inhibited TGF-β1-induced FN, Col I, and Col III synthesis in hVFFs. Our in vitro findings show that eupatilin effectively suppressed TGF-β1-induced fibrotic changes in hVFFs via the Smad and p38 signaling pathways. Thus, eupatilin may be considered a novel therapeutic agent for the treatment of vocal fold fibrosis.

show abstract

Eupatilin Alleviates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting Inflammation and Oxidative Stress

Cited by 23 publications

References 30 publications

Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells

Eupatilin Inhibits Reactive Oxygen Species Generation via Akt/NF-κB/MAPK Signaling Pathways in Particulate Matter-Exposed Human Bronchial Epithelial Cells

Isolation and characterization of methylated flavones from Artemisia kermanensis

Antifibrotic effects of eupatilin on TGF-β1-treated human vocal fold fibroblasts

Contact Info

Product

Resources

About