Eukaryotic Translation Initiation Factor 3 Subunit D is One Clinical Target and Pre-Tumor Gene for Non Hodgkin Lymphoma to Promote Cell Proliferation Through Warburg Effect by Interacting with GRP78

Abstract

Non-Hodgkin’s lymphoma (NHL) is one highly heterogeneous tumor originating from the lymphatic system. Its morbidity rate shows a gradually increasing trend year by year. The present study determined that the possible function and mechanisms of EIF3D on cell proliferation of NHL. A total of 24 patients with NHL were obtained at Aerospace Center Hospital. In patients with NHL, mRNA and protein expression of EIF3D was up-regulated. Patients with low EIF3D possessed better survival rate. EIF3D stimulated cell proliferation and the number of Edu cells through the activation of Warburg effect in vitro model of NHLNHL. Sh-EIF3D diminished NHL cell proliferation and the number of Edu cells through the inactivation of Warburg effect in vitro model of NHL. Then, we found that EIF3D reduced GRP78 protein ubiquitination to induce GRP78/Akt proteins, and si-EIF3D promote GRP78 protein ubiquitination to suppress GRP78/Akt proteins in vitro model of NHL. Our results indicate that EIF3D promote NHL cell proliferation throughWarburg effect by the inhibition of GRP78 protein ubiquitination, suggesting that it may prove to be one clinical target and pre-tumor gene for NHL.