Eukaryotic initiation factor 3, subunit C silencing inhibits cell proliferation and promotes apoptosis in human ovarian cancer cells

Fang, Wen; Wu, Zhimeng; Zhang, Qi-Zhu; Qin, Yuan-Kun; Zhou, Zunlun; Wu, Jin-Jian; Zhao, Xing; Tan, Jun; Sawmiller, Darrell; Zi, Dan

doi:10.1042/bsr20191124

Cited by 11 publications

(9 citation statements)

References 30 publications

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“…For example, EIF3A, the largest subunit of EIF3 family, has been found to be abnormally overexpressed in a variety of malignant tumors, which may be a promising therapeutic target for the design of anti-cancer drugs 28 . EIF3C was also demonstrated to participate in the regulation of human cancers such as ovarian cancer 29,30 , renal cell carcinoma 31 , osteosarcoma 32 , and cervical cancer 33 .…”

Section: Discussionmentioning

confidence: 99%

ZNF280A promotes lung adenocarcinoma development by regulating the expression of EIF3C

et al. 2021

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Lung adenocarcinoma (LUAD) is the most common histological subtype in non-small cell lung cancer, which is the malignant tumor with the highest mortality and morbidity in the world. Herein, ZNF280A, a member of the zinc finger protein family carrying two consecutive Cys2His2 zinc finger domains, was shown by us to act as a tumor driver in LUAD. The immunohistochemical analysis of ZNF280A in LUAD indicated its positive correlation with tumor grade, pathological stage and lymphatic metastasis, and negative relationship with patients’ survival. A loss-of-function study revealed the inhibition of LUAD development by ZNF280A in vitro and in vivo, whereas ZNF280A overexpression induced opposite effects. Statistical analysis of gene expression profiling in LUAD cells with or without ZNF280A knockdown identified EIF3C as a potential downstream of ZNF280A, which possesses similar regulatory effects on phenotypes of LUAD cells with ZNF280A. Moreover, downregulation of EIF3C in ZNF280A-overexpressed cells could attenuate neutralize the ZNF280A-induced promotion of LUAD. In summary, our study demonstrated that ZNF280A may promote the development of LUAD by regulating cell proliferation, apoptosis, cell cycle, and cell migration and probably via interacting EIF3C.

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Section: Discussionmentioning

confidence: 99%

ZNF280A promotes lung adenocarcinoma development by regulating the expression of EIF3C

et al. 2021

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“…Hypoxic melanoma EVs carried a hypoxic signature consisting of six proteins (AKR7A2, DDX39B, EIF3C, FARSA, PRMT5, VARS) which were significantly associated with a poor prognosis for melanoma patients. EIF3C promotes proliferation, migration and invasion of prostate [46], ovarian and hepatocellular carcinoma cells [47,48] and was associated with resistance to erlotinib in lung cancer [49]. PRMT5 (Protein arginine methyltransferase 5) is upregulated under hypoxia [50] and has many roles in cancer [51].…”

Section: Discussionmentioning

confidence: 99%

Hypoxia-Induced Adaptations of miRNomes and Proteomes in Melanoma Cells and Their Secreted Extracellular Vesicles

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Gaigneaux

et al. 2020

Cancers

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Reduced levels of intratumoural oxygen are associated with hypoxia-induced pro-oncogenic events such as invasion, metabolic reprogramming, epithelial–mesenchymal transition, metastasis and resistance to therapy, all favouring cancer progression. Small extracellular vesicles (EV) shuttle various cargos (proteins, miRNAs, DNA and others). Tumour-derived EVs can be taken up by neighbouring or distant cells in the tumour microenvironment, thus facilitating intercellular communication. The quantity of extracellular vesicle secretion and their composition can vary with changing microenvironmental conditions and disease states. Here, we investigated in melanoma cells the influence of hypoxia on the content and number of secreted EVs. Whole miRNome and proteome profiling revealed distinct expression patterns in normoxic or hypoxic growth conditions. Apart from the well-known miR-210, we identified miR-1290 as a novel hypoxia-associated microRNA, which was highly abundant in hypoxic EVs. On the other hand, miR-23a-5p and -23b-5p were consistently downregulated in hypoxic conditions, while the protein levels of the miR-23a/b-5p-predicted target IPO11 were concomitantly upregulated. Furthermore, hypoxic melanoma EVs exhibit a signature consisting of six proteins (AKR7A2, DDX39B, EIF3C, FARSA, PRMT5, VARS), which were significantly associated with a poor prognosis for melanoma patients, indicating that proteins and/or miRNAs secreted by cancer cells may be exploited as biomarkers.

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“…19 , 20 In addition, previous studies have demonstrated that inhibition of EIF3Cexpression can lead to inhibit cell proliferation and apoptosis. 21 , 22 However, the potential roles of EIF3Cin PC have not been fully understood.…”

Section: Discussionmentioning

confidence: 99%

Knockdown EIF3C Suppresses Cell Proliferation and Increases Apoptosis in Pancreatic Cancer Cell

et al. 2020

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Increasing evidence shows that eukaryotic initiation factor subunit (EIF3C) plays a crucial role in development of tumors. However, the underlying roles of EIF3Cin the development of pancreatic cancer (PC) remain unknown. In this study, we examined the expression of EIF3C in PC tissues, their adjacent normal tissues and 3 cell lines (SW1990, PANC-1 and AsPC-1). Moreover, the EIF3C-shRNA lentivirus was constructed to suppress EIF3C expression. Following this, the cell colony formation assay was employed to evaluate proliferation ability of PC cells. Meanwhile, the cell cycle and apoptotic assays were also performed by flow cytometry. We found that level of EIF3C in PC tissues was significantly increased compared with that in adjacent normal tissues. Furthermore, the knockdown of EIF3C can significantly reduce cell proliferation, block cell cycle in G2/M and induce apoptosis in both SW1990 and PANC-1 cells. Our findings suggest that EIF3C plays a crucial role in the progression of PC and may be a potential target in the treatment of PC.

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Eukaryotic initiation factor 3, subunit C silencing inhibits cell proliferation and promotes apoptosis in human ovarian cancer cells

Cited by 11 publications

References 30 publications

ZNF280A promotes lung adenocarcinoma development by regulating the expression of EIF3C

ZNF280A promotes lung adenocarcinoma development by regulating the expression of EIF3C

Hypoxia-Induced Adaptations of miRNomes and Proteomes in Melanoma Cells and Their Secreted Extracellular Vesicles

Knockdown EIF3C Suppresses Cell Proliferation and Increases Apoptosis in Pancreatic Cancer Cell

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