Eukaryotic expression plasmid transfer from the intracellular bacteriumListeria monocytogenesto host cells

Oral Immunization with RecombinantListeria monocytogenesControls Virus Load after Vaginal Challenge with Feline Immunodeficiency Virus

“…Additional advantages of L. monocytogenes are that the natural route of infection is at the intestinal mucosa and that antigen-presenting cells are target cells. Furthermore, it has been proven that L. monocytogenes can transfer a plasmid to mammalian cells (21,32) and, as reported here, can be employed to deliver an FIV Env-expressing DNA vaccine plasmid. As with any live vaccine vector, safety issues must be addressed.…”

“…As a vector, foreign genes can be stably inserted into the L. monocytogenes genome and express at high levels into the cytoplasm of host cells for processing and presentation by the major histocompatibility complex class I pathway (27). L. monocytogenes is also an effective delivery vehicle for plasmids designed for eukaryotic expression of immunogens (21,32). With regard to safety, several strategies have been employed to restrict but not eliminate replication of L. monocytogenes, thereby allowing amplification of the antigenic load and induction of an immune response at the mucosa as well as systemically (25,40).…”

Requirement of theListeria monocytogenesBroad-Range Phospholipase PC-PLC during Infection of Human Epithelial Cells

“…During infection of the humanderived epithelial cell line Henle 407, PC-PLC promotes lysis of primary vacuoles in the absence of LLO (31). Previous studies have shown that LLO-negative L. monocytogenes strains can also escape from primary vacuoles in the humanderived epithelial cell lines HEp-2 and HeLa (24,40). However, an L. monocytogenes strain with deletions of LLO and both phospholipases, PI-PLC and PC-PLC, fails to escape from the primary vacuole in these cells (24,40).…”

“…Previous studies have shown that L. monocytogenes can access the host cell cytosol in the absence of LLO during infection of the human-derived fibroblast cell line WS1, the human-derived epithelial cell line Henle 407, and human-derived dendritic cells (42,44). Recently, the epithelial cell lines HEp-2 and HeLa have also been identified as human-derived host cells in which LLO is not required for lysis of L. monocytogenes-containing primary vacuoles (24,40). Prior studies have shown that PC-PLC mediates LLO-independent escape from primary vacuoles in Henle 407 cells (31).…”

Bactofection of lung epithelial cells in vitro and in vivo using a genetically modified Escherichia coli

“…Proof-of-principle for bactofection in a range of non-phagocytic cell lines, including airway epithelial cells, has been established in vitro. 13,14,[17][18][19] These studies have mainly focused on transfer of the GFP reporter gene. However, more relevant to our work, recombinant E. coli and L. monocytogenes were recently used to transfer artificial chromosomes carrying the CFTR gene locus 20 or a pCMV-CFTR plasmid, 10,21 respectively, to cell lines in vitro.…”