Eukaryotic elongation factor 2 is involved in the anticoccidial action of diclazuril in the second-generation merozoites of Eimeria tenella

Zhou, Bian-hua; Jia, Liu-shu; Guo, H. W.; Ding, Haiying; Yang, Jing-yun

doi:10.1016/j.vetpar.2019.108991

Cited by 16 publications

(14 citation statements)

References 47 publications

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“…We infer that the eEF2 protein (A0A086JND6_TOXGO) and its corresponding peptide sequence (NMSVIAHVDHGK) may be meaningful in future research. We noted that eEF2 plays a major role in the protein synthesis and survival of Eimeria tenella [ 33 ], and low levels of total eEF2 protein in T. gondii reduced TgPHYb expression [ 34 ]. eEF2 has been identified or confirmed as an antimalarial and anticoccidial drug target of Plasmodium falciparum and Eimeria tenella , respectively [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…We noted that eEF2 plays a major role in the protein synthesis and survival of Eimeria tenella [ 33 ], and low levels of total eEF2 protein in T. gondii reduced TgPHYb expression [ 34 ]. eEF2 has been identified or confirmed as an antimalarial and anticoccidial drug target of Plasmodium falciparum and Eimeria tenella , respectively [ 33 , 34 ]. It is clear that the eEF2 of T. gondii localizes to the nucleus [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

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Glycosylation Analysis of Feline Small Intestine Following Toxoplasma gondii Infection

Zhai

Xie

Peng

et al. 2022

Animals

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Toxoplasma gondii (T. gondii) is responsible for severe human and livestock diseases, huge economic losses, and adversely affects the health of the public and the development of animal husbandry. Glycosylation is a common posttranslational modification of proteins in eukaryotes, and N-glycosylation is closely related to the biological functions of proteins. However, glycosylation alterations in the feline small intestine following T. gondii infection have not been reported. In this study, the experimental group was intragastrically challenged with 600 brain cysts of the Prugniuad (Pru) strain that were collected from infected mice. The cats’ intestinal epithelial tissues were harvested at 10 days post-infection and then sent for protein glycosylation analysis. High-performance liquid chromatography coupled to tandem mass spectrometry was used to analyze the glycosylation alterations in the small intestine of cats infected with T. gondii. The results of the present study showed that 56 glycosylated peptides were upregulated and 37 glycosylated peptides were downregulated in the feline small intestine infected by T. gondii. Additionally, we also identified eight N-glycosylated proteins of T. gondii including eight N-glycopeptides and eight N-glycosylation sites. The protein A0A086JND6_TOXGO (eEF2) and its corresponding peptide sequence were identified in T. gondii infection. Some special GO terms (i.e., cellular process and metabolic process, cell and cell part, and catalytic activity) were significantly enriched, and the Clusters of Orthologous Groups of proteins (COG) function prediction results showed that posttranslational modification, protein turnover, and chaperones (11%) had the highest enrichment for T. gondii. Interestingly, eEF2, a protein of T. gondii, is also involved in the significantly enriched T. gondii MAPK pathway. The host proteins ICAM-1 and PPT1 and the endoplasmic reticulum stress pathway may play an important role in the glycosylation of Toxoplasma-infected hosts. This is the first report showing that T. gondii oocysts can undergo N-glycosylation in the definitive host and that eEF2 is involved, which may provide a new target for T. gondii detection to prevent the spread of T. gondii oocysts in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Glycosylation Analysis of Feline Small Intestine Following Toxoplasma gondii Infection

Zhai

Xie

Peng

et al. 2022

Animals

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“…The eukaryotic elongation factor 2 (eEF2) is important for protein synthesis and cell survival. 45 This protein has been suggested as a potential drug target in several parasitic infestations, for example poultry coccidiosis 45 and malaria. 46 Also, this protein has served as a vaccine candidate, particularly in leishmaniasis.…”

Section: Discussionmentioning

confidence: 99%

Immunoproteomic analysis of fish ectoparasite, Argulus siamensis antigens

Das

Badhe

Sahoo

et al. 2021

Parasite Immunology

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Aquaculture production makes a considerable contribution to the economy of developing countries. The growth of aquaculture is constrained due to different infectious diseases caused by bacteria, viruses and parasites. Among different parasitic diseases, argulosis, a disease caused by a crustacean ectoparasite of the genus Argulus causes heavy economic losses to aquaculture production.Argulus spp. are the cosmopolitan fish parasites commonly known as fish lice with about 129 species distributed throughout the world. 1 Depending upon species, these parasites live on a wide range of freshwater or marine fish species as hosts for the most part of their life cycle and the intensity of infections are higher on cultured fish than in the natural environment. 2 These ectoparasites feed on blood as well as on mucus and epithelial cells by puncturing the host's skin by the pre-oral stylet and injecting a cytolytic toxin. 3 This results in primary lesions at the contact sites. Haemorrhagic spots develop particularly on the fins and at the base of fins, the head, and nonrespiratory surfaces of the gill cavity, which may lead to secondary infections. 4 Fish loss usually occurs due to direct effects such as dermal ulceration, osmotic imbalance, physiological stress and immunosuppression. 5

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“…We now show experimental results for a proof-ofconcept demonstration of the algorithm introduced in the previous section, on a 16-qubit quantum computer provided by IBM Q Experience. We perform unsupervised data clustering for three sets of paper abstracts from three different fields: quantum physics [84][85][86], medicine [87][88][89] and biology [90][91][92]. Each set consists of three papers selected at random that focus on one of the following topics: "Quantum Machine Learning", "Cancer" and "Gene Expression".…”

Section: Experimental Demonstration Of Qasofmmentioning

confidence: 99%

Hybrid quantum-classical unsupervised data clustering based on the Self-Organizing Feature Map

Lazarev¹,

Narozniak²,

Byrnes³

et al. 2020

Preprint

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Eukaryotic elongation factor 2 is involved in the anticoccidial action of diclazuril in the second-generation merozoites of Eimeria tenella

Cited by 16 publications

References 47 publications

Glycosylation Analysis of Feline Small Intestine Following Toxoplasma gondii Infection

Glycosylation Analysis of Feline Small Intestine Following Toxoplasma gondii Infection

Immunoproteomic analysis of fish ectoparasite, Argulus siamensis antigens

Hybrid quantum-classical unsupervised data clustering based on the Self-Organizing Feature Map

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