Eukaryal and archaeal diversity in a submerged sinkhole ecosystem influenced by sulfur-rich, hypoxic groundwater

Nold, Stephen C.; Zajack, Heidi A.; Biddanda, Bopaiah A.

doi:10.1016/j.jglr.2010.02.014

Cited by 20 publications

(25 citation statements)

References 73 publications

(90 reference statements)

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“…There are currently no cultured representatives or proposed phenotypes for these groups. Cluster B and C sequences have been retrieved from environments with methanogenic communities and complete or partially anoxic zones, such as water [48], landfill leachates [49], sediments [50], bioreactors [51] and the digestive tract of animals [52]. This study adds activated sludge to that list.…”

Section: Discussionmentioning

confidence: 99%

Diversity and dynamics of Archaea in an activated sludge wastewater treatment plant

2012

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BackgroundThe activated sludge process is one of the most widely used methods for treatment of wastewater and the microbial community composition in the sludge is important for the process operation. While the bacterial communities have been characterized in various activated sludge systems little is known about archaeal communities in activated sludge. The diversity and dynamics of the Archaea community in a full-scale activated sludge wastewater treatment plant were investigated by fluorescence in situ hybridization, terminal restriction fragment length polymorphism analysis and cloning and sequencing of 16S rRNA genes.ResultsThe Archaea community was dominated by Methanosaeta-like species. During a 15 month period major changes in the community composition were only observed twice despite seasonal variations in environmental and operating conditions. Water temperature appeared to be the process parameter that affected the community composition the most. Several terminal restriction fragments also showed strong correlations with sludge properties and effluent water properties. The Archaea were estimated to make up 1.6% of total cell numbers in the activated sludge and were present both as single cells and colonies of varying sizes.ConclusionsThe results presented here show that Archaea can constitute a constant and integral part of the activated sludge and that it can therefore be useful to include Archaea in future studies of microbial communities in activated sludge.

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Section: Discussionmentioning

confidence: 99%

Diversity and dynamics of Archaea in an activated sludge wastewater treatment plant

2012

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“…Benthic producers were separated in the field by color and morphology, as previous work indicated distinct lineages based on color differences: purple filamentous cyanobacteria, whitish strands of sulfur-oxidizing bacteria (Beggiatoa spp. ), and green photosynthetic benthic algae (Ruberg et al 2008, Biddanda et al 2009, Nold et al 2010a, 2010b. For macrophytic vegetation, we made a visual estimate of relative abundance and collected samples from the dominant vegetation types.…”

Section: Methodsmentioning

confidence: 99%

Benthic macroinvertebrate and fish communities in Lake Huron are linked to submerged groundwater vents

Sanders¹,

Biddanda²,

Stricker³

et al. 2011

Aquat. Biol.

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“…However, the DSAG is not restricted to the marine environment, as can be concluded from several recent studies reporting their occurrence in inland lakes (Dong et al, 2006; Jiang et al, 2008; Nold et al, 2010; Schubert et al, 2011), a terrestrial cave system (Chen et al, 2009), soil (Kasai et al, 2005), and fresh water iron mats (Kato et al, 2012). Although members of the DSAG apparently have a liberal habitat preference, there is at least one common denominator: they seem to be restricted to anaerobic or micro-aerophilic environments.…”

Section: Introductionmentioning

confidence: 97%

Quantitative and phylogenetic study of the Deep Sea Archaeal Group in sediments of the Arctic mid-ocean spreading ridge

Jørgensen¹,

Thorseth²,

Pedersen³

et al. 2013

Front. Microbiol.

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In marine sediments archaea often constitute a considerable part of the microbial community, of which the Deep Sea Archaeal Group (DSAG) is one of the most predominant. Despite their high abundance no members from this archaeal group have so far been characterized and thus their metabolism is unknown. Here we show that the relative abundance of DSAG marker genes can be correlated with geochemical parameters, allowing prediction of both the potential electron donors and acceptors of these organisms. We estimated the abundance of 16S rRNA genes from Archaea, Bacteria, and DSAG in 52 sediment horizons from two cores collected at the slow-spreading Arctic Mid-Ocean Ridge, using qPCR. The results indicate that members of the DSAG make up the entire archaeal population in certain horizons and constitute up to ~50% of the total microbial community. The quantitative data were correlated to 30 different geophysical and geochemical parameters obtained from the same sediment horizons. We observed a significant correlation between the relative abundance of DSAG 16S rRNA genes and the content of organic carbon (p < 0.0001). Further, significant co-variation with iron oxide, and dissolved iron and manganese (all p < 0.0000), indicated a direct or indirect link to iron and manganese cycling. Neither of these parameters correlated with the relative abundance of archaeal or bacterial 16S rRNA genes, nor did any other major electron donor or acceptor measured. Phylogenetic analysis of DSAG 16S rRNA gene sequences reveals three monophyletic lineages with no apparent habitat-specific distribution. In this study we support the hypothesis that members of the DSAG are tightly linked to the content of organic carbon and directly or indirectly involved in the cycling of iron and/or manganese compounds. Further, we provide a molecular tool to assess their abundance in environmental samples and enrichment cultures.

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Eukaryal and archaeal diversity in a submerged sinkhole ecosystem influenced by sulfur-rich, hypoxic groundwater

Cited by 20 publications

References 73 publications

Diversity and dynamics of Archaea in an activated sludge wastewater treatment plant

Diversity and dynamics of Archaea in an activated sludge wastewater treatment plant

Benthic macroinvertebrate and fish communities in Lake Huron are linked to submerged groundwater vents

Quantitative and phylogenetic study of the Deep Sea Archaeal Group in sediments of the Arctic mid-ocean spreading ridge

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