Eugenol, a plant-derived phenolic nutraceutical, protects thiol (SH) group in myocardium from ROS-mediated oxidation under chemotherapeutic stress induced by arsenic trioxide – ain vivomodel study

Abstract

Arsenic trioxide is an effective chemotherapeutic agent for the treatment of acute promyelocytic leukemia. The clinical usefulness of arsenic trioxide is narrow due to different organ toxicities. It is hypothesized that the generation of reactive oxygen species by arsenic trioxide leads to thiol-based oxidative damage in rat myocardium. In this study, the defensive effect of eugenol on thiol-based oxidative stress was investigated in arsenic trioxide-treated rats. Rats were orally administered with arsenic trioxide (4 mg/kg per day) alone and in combination with eugenol (5 mg/kg per day) for 30 days. Reduction in relative organ weight, total thiol level, protein thiol content, acid-soluble thiol content, thioredoxin activity, and protein content was witnessed in arsenic trioxide-treated rats. Additionally, the total antioxidant activity, tissue GSH level, and GSH/GSSG ratio were considerably diminished. However, the co-treatment of eugenol noticeably sheltered the arsenic trioxide-mediated cardiotoxicity. In conclusion, eugenol is a prospective phenolic compound, of natural origin, for protecting the thiol group in myocardium from oxidative stress by chemotherapeutic compounds.

In Vitro and In Vivo Protective Effects of Lentil (Lens culinaris) Extract against Oxidative Stress-Induced Hepatotoxicity

“…Reportedly, polyphenol-rich plants and polyphenolic compounds, such as flavonoids, carotenoids, and phenolic acid, exhibit protective effects against oxidative stress-induced hepatotoxicity by either the direct scavenging of ROS or the modulation of endogenous antioxidant defense system [ 21 , 22 , 23 ]. More recently, a study was conducted to elucidate the profile of phytochemical constituents in 20 different lentil cultivars and their antioxidant activities, and the results revealed that the content of total tocopherols and total carotenoids were 37–64 μg/g dry weight (DW) and 5.3–28.1 μg/g DW, respectively [ 24 ].…”

Eugenol attenuates concanavalin A-induced hepatitis through modulation of cytokine levels and inhibition of mitochondrial oxidative stress

“…Recently, Binu et al [28] showed that eugenol restored liver function from arsenic-induced liver injury. Further, the antioxidant effects of eugenol have been highlighted in various disease models, proving its versatility [29,30].…”

<p>Protective Effects of Crocetin on Arsenic Trioxide-Induced Hepatic Injury: Involvement of Suppression in Oxidative Stress and Inflammation Through Activation of Nrf2 Signaling Pathway in Rats</p>

“…13,14 Synthetic or natural scavengers of ROS and antioxidants could attenuate ATO-induced toxicity, thereby enabling full exploitation of the therapeutic potential of ATO. 15 The imbalance between the generation of ROS and the antioxidant systems is usually maintained by critical enzymes, for example, superoxide dismutase (SOD) and catalase (CAT). 13 Exposure of arsenic to individuals involved in the production of pro-inflammatory cytokines.…”