Eugenia uniflora leaves essential oil induces toxicity in Drosophila melanogaster: involvement of oxidative stress mechanisms

The present study aims to test the Acritopappus confertus (Gardner) R. M. King & H. Rob. essential oil allelopathic potential on the germination and inital development of Cenchrus echinatus L. and Lactuca sativa L. seedlings, in addition to identifying and quantifying chemical constituents. The chemical composition analysis was performed by gas chromatography (GC-FID) and gas chromatography coupled to mass-spectrometry (GC-MS). For the allelopathic assays, the essential oil was emulsified with dimethylsulfoxide (DMSO) in a 1:1 ratio, and diluted in distilled water to obtain 0.001, 0.01, 0.10, 0.25, 0.50, 0.75 and 1% c. The control consisted of 1% aqueous DMSO solution.Treatments were performed in five repetition with 20 seeds each. The pH of the oils were measured and adjusted to a scale of 6 to 7. Assays were conducted in a germination chamber at 25 °C with a 12h photoperiod. Seed germination, Germination Speed Index (GSI), caulicle and radicle length were analyzed. The data were subjected to analysis of variance and the means were compared by Tukey test (p<0.05), through the ASSISTAT. The constituents mycene, β-pinene and limonene stood out the most in chemical analysis. The essential oil did not influence C. echinatus and L. sativa seed germination, however it influenced GSI and seedling development. The effects observed herein may be due to chemical constituents found in the studied species, which may act in an isolated or combined manner.

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“…The temperature program was the same as that used for GC analyzes. The Essential oil injection volume was 1 μL (Cunha et al, 2015).…”

Section: Gas Chromatography Coupled To Mass Spectrometry (Gc-ms)mentioning

confidence: 99%

Chromatographic profile and allelopathic potential of the essential oil of Acritopappus confertus (Gardner) R. M. King & H. Rob. (Asteraceae)

Sousa

Leite

Linhares

et al. 2020

RSD

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“…At the cellular level, essential oils and their components may exert prooxidant effects by changes in electron flow through mitochondrial complexes and increasing reactive oxygen species (ROS) production which cause damages to macromolecules and apoptosis [56]. For example, prooxidant effects of EO from Eugenia uniflora (L.) leaves on Drosophila melanogaster were confirmed by elevation in ROS and lipid peroxides levels, as well as by activation of enzymatic antioxidants and proteins involved in stress response and antioxidant signaling [57].…”

Section: Introductionmentioning

confidence: 99%

Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus

Lazarević

Jevremović²,

Kostić

et al. 2020

Insects

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The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.

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“…Chromatographic conditions were as follows: initial oven temperature was 50°C for one minute, followed by heating at 50°C/min to 300°C, keeping this temperature for 9 minutes, for a total of 60 minutes. Separation was achieved on a DB-5MS column (30 m x 320 μ m x 0.25 μ m) at a constant rate of 1.5 mL/min of helium; detection was performed in quadrupole equipment using ionizing electrons [ 6 ]. Identification of components was done based on the retention index (RI), determined by the homologous series of n-alkanes, C7-C30 under similar experimental conditions, compared with the search mass spectra libraries (NIST and WILEY) and mass spectra data in the literature [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

Immunotoxicological Evaluation of Schinus molle L. (Anacardiaceae) Essential Oil in Lymphocytes and Macrophages

Duarte

Zambrano

Quintana

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Schinus molle L. is used to treat various diseases; however, the literature lacks information regarding its possible immunotoxic effects. The aim of the study was to investigate the immunotoxic effects of essential oil from leaves of Schinus molle L. in cultures of human lymphocytes and macrophages. The cultures were treated with essential oil (EO) of Schinus molle L. and subsequently subjected to genotoxic analysis (comet assay), mutagenic analysis (micronucleus frequency and chromosomal aberration), and cytotoxic (cell viability) and functional parameters (interleukins secretions). Our analyses have determined that the essential oil from leaves of Schinus molle L. presents several compounds with α-pinene being the major compound; in addition, the compound verbenene was firstly identified; genotoxic effects were detected only in macrophages and only at the two highest concentrations tested. An important finding is that Schinus molle L. oil causes an activation of the immune system. This action has its mechanism centered by the cascade nitric oxide-interleukin-10-tumor necrosis factor alpha.

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Eugenia uniflora leaves essential oil induces toxicity in Drosophila melanogaster: involvement of oxidative stress mechanisms

Abstract: Eugenia unifloraL. (Myrtaceae family), also known as “pitanga”, is a tree species widely used in popular medicine.

Cited by 51 publications

References 49 publications

Chromatographic profile and allelopathic potential of the essential oil of Acritopappus confertus (Gardner) R. M. King & H. Rob. (Asteraceae)

Chromatographic profile and allelopathic potential of the essential oil of Acritopappus confertus (Gardner) R. M. King & H. Rob. (Asteraceae)

Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus

Immunotoxicological Evaluation of Schinus molle L. (Anacardiaceae) Essential Oil in Lymphocytes and Macrophages

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