Eubacterium tucumanense sp. nov.: an Anaerobic Gram-positive Non-sporeformer Isolated from an Anaerobic Digester

Diaz, Hector F.; Núñez, Carlos Germán; Siñeríz, Faustino

doi:10.1099/00221287-135-9-2537

Cited by 1 publication

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References 12 publications

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“…Growth was determined by measuring the OD 600 using a UV/visible spectrophotometer (SPECORD205; Analytic Jena). The temperature range for growth was determined at 16,30,37,40,45 and 65 °C and cell cultures were incubated for 3 days anaerobically. The pH range for growth was tested at pH 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0, and cell cultures were incubated for 3 days at 37 °C anaerobically, by adjusting the pH before sterilisation with 0.1 M sodium acetate buffer (for pH 4.0-6.0), 0.1 M phosphate buffer (for pH 7.0-8.0) and 0.2 M Tris buffer (for pH 8.0-9.0).…”

Section: Cell Morphology Observation and Chemotaxonomic Determinationsmentioning

confidence: 99%

Eubacterium album sp. nov., a butyrate-producing bacterium isolated from faeces of a healthy human

Liu,

Abdugheni,

Ran

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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An oval to rod-shaped, Gram-stain-positive, strictly anaerobic bacterium, designated LFL-14T, was isolated from the faeces of a healthy Chinese woman. Cells of the strain were non-spore-forming, grew optimally at 37 °C (growth range 30–45 °C) and pH 7.0 (growth range 6.0–9.0) under anaerobic conditions in the liquid modified Gifu anaerobic medium (mGAM). The result of 16S rRNA gene-based analysis indicated that LFL-14T shared an identity of 94.7 0% with Eubacterium ventriosum ATCC 27560T, indicating LFL-14T represented a novel taxon. The results of genome-based analysis revealed that the average nucleotide identity (ANI), the digital DNA–DNA hybridisation (dDDH) and average amino acid identity (AAI) between LFL-14T and its phylogenetically closest neighbour, Eubacterium ventriosum ATCC 27560T, were 77.0 %, 24.6 and 70.9 %, respectively, indicating that LFL-14T represents a novel species of the genus Eubacterium. The genome size of LFL-14T was 2.92 Mbp and the DNA G+C content was 33.14 mol%. We analysed the distribution of the genome of LFL-14T in cohorts of healthy individuals, type 2 diabetes patients (T2D) and patients with non-alcoholic fatty liver disease (NAFLD). We found that its abundance was higher in the T2D cohort, but it had a low average abundance of less than 0.2 % in all three cohorts. The percentages of frequency of occurrence in the T2D, healthy and NAFLD cohorts were 48.87 %, 16.72 % and 13.10 % respectively. The major cellular fatty acids of LFL-14T were C16 : 0 (34.4 %), C17 : 0 2-OH (21.4 %) and C14 : 0 (11.7 %). Additionally, the strain contained diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE), as well as unidentified phospholipids and unidentified glycolipids. The glucose fermentation products of LFL-14T were acetate and butyrate. In summary, On the basis of its chemotaxonomic, phenotypic, phylogenetic and phylogenomic properties, strain LFL-14T (= CGMCC 1.18005T = KCTC 25580T) is identified as representing a novel species of the genus Eubacterium, for which the name Eubacterium album sp. nov. is proposed.

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Section: Cell Morphology Observation and Chemotaxonomic Determinationsmentioning

confidence: 99%

Eubacterium album sp. nov., a butyrate-producing bacterium isolated from faeces of a healthy human

Liu,

Abdugheni,

Ran

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

show abstract

Eubacterium tucumanense sp. nov.: an Anaerobic Gram-positive Non-sporeformer Isolated from an Anaerobic Digester

Cited by 1 publication

References 12 publications

Eubacterium album sp. nov., a butyrate-producing bacterium isolated from faeces of a healthy human

Eubacterium album sp. nov., a butyrate-producing bacterium isolated from faeces of a healthy human

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