Etude ultrastructurale des lésions viscérales provoquées par l’injection intra-péritonéale de <i>Trichomonas vaginalis</i> chez la souris

Brugerolle, Guy; Gobert, J.G.; Savel, J

doi:10.1051/parasite/1974493301

Cited by 13 publications

(17 citation statements)

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“…In spite of these observations, we have shown that amoeboid forms of T. vaginalis (Figs. 1, 2, 4, 5) can be found in vitro and in situations distinct from previous reports (Brugerolle et al 1974;Nielsen and Nielsen 1975;Rasmussen et al 1986;Honigberg and Brugerolle 1990;Arroyo et al 1993;Alderete et al 1995;Petrin et al 1998;Gilbert et al 2000). Here we demonstrate that a fresh T. vaginalis isolate (FMV1 strain) presents amoeboid forms even after axenic subculturing for many weeks (Fig.…”

Section: Discussioncontrasting

confidence: 45%

Trichomonas vaginalis virulence against epithelial cells and morphological variability: the comparison between a well-established strain and a fresh isolate

et al. 2004

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The FMVI strain of Trichomonas vaginalis was freshly isolated from an asymptomatic patient, and its morphological properties and virulence in vitro compared with the well-established JT strain. The morphological variability of the parasites was assessed by differential interference microscopy and both scanning and transmission electron microscopy. The FMV1 strain presented nearly 20% amoeboid cells whereas the JT strain presented high percentages of ellipsoid but no amoeboid cells. The FMV1 morphotype population was unaltered after at least 1 year of subculturing. Electron microscopy revealed that this strain produced numerous pseudopod structures which mediated intimate contact and interdigitation among trophozoites. Dead FMV1 parasites were often phagocytosed by conspecific cells. We also compared the cytolytic capacity of these two populations against epithelial MDCK cells and its contact dependence. The FMV1 strain rapidly adhered to plastic or glass surfaces and to MDCK monolayers. This strain destroyed about 93% of the epithelial cells in 90 min whereas the cytolytic activity of the JT parasites was very much lower (about 41%). Parasite supernatants displayed no cytolytic activity, indicating contact-mediated lysis. The protozoan virulence in vitro did not correlate well with the clinical observations. The implications of these results are discussed.

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Section: Discussioncontrasting

confidence: 45%

Trichomonas vaginalis virulence against epithelial cells and morphological variability: the comparison between a well-established strain and a fresh isolate

et al. 2004

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“…Experimental infections of a variety of mammalian hosts with T. vaginalis have been used to study various aspects of the host-parasite interactions, to evaluate the inherent pathogenicity of the parasite and to assay the effects of antitrichomonal drugs (Brugerolle et al 1974;Honigberg 1978;Street et al 1983;Cerkasovova et al 1986). Most data on the experimental infection of laboratory animals with T. vaginalis, however, have been obtained from subcutaneous and intraperitoneal inoculation of mice with live trichomonads (Frost et al 1961;Honigberg 1961;Reardon et al 1961;Frost and Honigberg 1962;Teras and Roigas 1966).…”

Section: Introductionmentioning

confidence: 99%

Relationship of the virulence of Trichomonas vaginalis and the major histocompatibility complex in murine trichomonad infection

Caterina

Warton

Papadimitriou

et al. 1996

Parasitology Research

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A biological assay based upon the induction of abscesses in mice injected subcutaneously with Trichomonas vaginalis was shown to be a valid method for comparing the virulence of two isolates of T. vaginalis cultured from two patients, one suffering from severe vaginitis and the other exhibiting only mild disease. The data showed excellent correlation between the physical dimensions of abscesses in mice injected with each trichomonad isolate and the severity of vaginitis produced in the women from whom the isolates were obtained. The assay employed in our study incorporated measurement of the mean abscess volumes from day 1 to day 6 post-inoculation with T. vaginalis. We found that the abscess assay was clearly superior to a murine intraperitoneal assay for virulence evaluation of trichomonad isolates. We then used the murine abscess assay to determine the susceptibility of different strains of mice to infection with a virulent T. vaginalis isolate so as to test whether the genetic constitution of the host would influence the pathogenesis of the disease. BALB/c (H-2d) mice were susceptible to infection with T. vaginalis, but both CBA/CaH (H-2k) and BALB/c-H-2k mice were shown to be resistant. The quantitation of abscess formation in these inbred and congeneic resistant mouse strains demonstrates that the severity of infection with T. vaginalis is governed by genes mapping within the major histocompatibility complex.

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“…The presence of interdigitations in the contact area and the reduced intercellular space in contact areas are also well shown (Brugerolle et al 1974;Nielsen and Nielsen 1975). On the other hand, microchannels through which microvilli and cytoplasmic fragments of epithelial cells have been internalized have been suggested to exist (GonzaÂ lez- Robles et al 1995).…”

Section: Discussionmentioning

confidence: 93%

“…Extensive studies had previously been performed to clarify basic aspects of interaction between Trichomonas vaginalis and cells from the host (Brugerolle et al 1974;Nielsen and Nielsen 1975;Heath 1981;Alderete and Pearlman 1984;Krieger et al 1985;Rasmussen et al 1986;Silva Filho and De Souza 1988;Krieger 1990;GonzaÂ lez-Robles et al 1995). However, the mechanism responsible for the cytopathic eect had not yet been clari®ed.…”

Section: Discussionmentioning

confidence: 99%

Observation of membrane fusion on the interaction of Trichomonas vaginalis with human vaginal epithelial cells

Furtado

Benchimol

1998

Parasitology Research

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The in vitro cytopathic effect of Trichomonas vaginalis on epithelial cells was analyzed through the interaction of two parasite strains with freshly collected human vaginal epithelial cells (HVECs) from normal women. Videomicroscopy, light and electron microscopy (scanning and transmission), freeze-fracture, the tracer lanthanum nitrate, and the periodic acid-thio-semicarbazide-silver proteinate techniques were used to analyze regions of close contact between the HVECs and T. vaginalis. After 2 h of interaction, all HVECs were dead, whereas all the trichomonads were alive. Microscopic observations demonstrated that in addition to previously described regions of adhesion and interdigitations, areas of continuity between the cytoplasm of the two interacting cells were found. They were not easy to find since they correspond to focal spots placed in different depths of the section. When these regions were depicted, the plasma membranes of the T. vaginalis and the vaginal epithelial cells seemed to be fused.

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Etude ultrastructurale des lésions viscérales provoquées par l’injection intra-péritonéale de Trichomonas vaginalis chez la souris

Cited by 13 publications

References 1 publication

Trichomonas vaginalis virulence against epithelial cells and morphological variability: the comparison between a well-established strain and a fresh isolate

Trichomonas vaginalis virulence against epithelial cells and morphological variability: the comparison between a well-established strain and a fresh isolate

Relationship of the virulence of Trichomonas vaginalis and the major histocompatibility complex in murine trichomonad infection

Observation of membrane fusion on the interaction of Trichomonas vaginalis with human vaginal epithelial cells

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