Etude in vitro des phenomenes immunologiques dans la schistosomiase humaine et experimentale—I. Etude comparative in vitro de l'activite lethale d'immunserums sur les formes immatures et sur les adultes de Schistosoma mansoni

Càpron, André; Capron, Moníque; Dupas, H; Bout, Daniel; Petitprez, A

doi:10.1016/0020-7519(74)90026-5

Cited by 45 publications

(14 citation statements)

References 9 publications

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“…Contrasting with the inefficiency of T cell-mediated killing, it was shown early that antischistosome antibodies can damage schistosomula in vitro. The initial observation made with Rhesus monkey immune serum, which led to the identification of an IgG antibody lethal for schistosomula in the presence of complement (Clegg & Smithers 1972) was rapidly followed by several items of evidence that complement-fixing antibodies could achieve a highly significant killing ofschistosomula in various animal species including man, rabbit, rat and mouse (Capron et al 1974, Capron et al 1977d).…”

Section: B) Lethal Antibodymentioning

confidence: 99%

Effector Mechanisms of Immunity to Schistosomes and Their Regulation

Càpron

Dessaint

Capron

et al. 1982

Immunological Reviews

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108

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Section: B) Lethal Antibodymentioning

confidence: 99%

Effector Mechanisms of Immunity to Schistosomes and Their Regulation

Càpron

Dessaint

Capron

et al. 1982

Immunological Reviews

Self Cite

108

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“…Schistosomulum antigen was prepared by sonication of frozen thawed mechanical schistosomula [19,20], Soluble egg antigen (SEA) was prepared according to the method of Boros and Warren …”

Section: Crude Antigenic Preparationsmentioning

confidence: 99%

T-Cell Responsiveness towards Various Synthetic Peptides of the P28 Antigen in Rat and Mouse Models during <i>Schistosoma mansoni</i> Infection

Wolowczuk

Auriault²,

Gras-Masse³

et al. 1990

Int Arch Allergy Immunol

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It has recently been demonstrated that the Schistosoma mansoni P28 antigen can induce a strong protective immunity after direct immunization in various experimental models. T lymphocytes from Fischer rats immunized with the recombinant P28 antigen were cultured in vitro in the presence of seven synthetic peptides derived from the amino acid sequence of the P28. The most significant and reproducible proliferation was obtained with the 24–43 and 115–131 synthetic peptides. In order to analyze whether these located determinants were also exposed to the host’s immune system during the natural S. mansoni infection or after immunization with crude antigenic extracts of various development stages of the parasite, the T-cell responsiveness of infected or immunized Fischer rats and BALB/c mice was tested towards these synthetic peptides. The results showed that, in both permissive (mouse) and non-permissive (rat) hosts, 24–43 and 115–131 synthetic peptides are recognized during the course of infection and that there is a dynamic variation of this recognition. These peptides are also recognized by T cells educated against crude antigenic extracts of different developmental stages of the parasite which contained the native form of the P28 molecule. Taken together, the results indicated that these synthetic peptides derived from the recombinant P28 antigen can activate T lymphocytes educated against the native P28 molecule during the development and maturation of the parasite in their hosts. Therefore, they might be useful for the construction of synthetic vaccines against schistosomiasis.

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“…S. mansoni schistosomula prepared according to the skin penetration procedure, were the targets of the cytotoxicity assays (20). For IgE-mediated eosinophildependent cytotoxicity, 50-#1 aliquots of the schistosomula suspension containing 50 targets in M EM supplemented with 1% heat-inactivated FCS (MEM/FCS), were incubated in flat-bottomed microtiter plates (Nunc, Roskilde, Denmark).…”

Section: Methodsmentioning

confidence: 99%

Functional study of a monoclonal antibody to IgE Fc receptor (Fc epsilon R2) of eosinophils, platelets, and macrophages.

Capron¹,

Jouault²,

Prin³

et al. 1986

The Journal of Experimental Medicine

141

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An IgM mAb (BB10) was produced by immunization of mice with human eosinophils purified according to their abnormal low density ("hypodense" cells), and previously shown to exhibit increased IgE-dependent antiparasite cytotoxicity. This BB10 antibody, selected for positive fluorescence staining of hypodense blood or lung eosinophils and low or negative staining of normodense eosinophils or neutrophils, could strongly inhibit IgE-dependent cytotoxicity of human eosinophils and platelets. The specificity for the IgE Fc receptor was suggested by the high levels of inhibition of IgE rosettes formed by eosinophils after incubation with the purified IgM fraction of BB10, whereas other receptors (Fc gamma R, CR1) were not affected. On the other hand, BB10, able to inhibit rat eosinophil Fc epsilon R, did not react with the IgE Fc receptor on mast cells or basophils. A technique using radioiodinated BB10 allowed us to quantify the specific binding of BB10 to human eosinophils and platelets. Competition experiments revealed a crossinhibition between the binding of BB10 and IgE, suggesting the specificity of BB10 for the IgE binding site of eosinophil, platelet, and monocyte Fc epsilon R. Three proteins having extrapolated Mr of 32,000, 43,000-45,000, and 97,000 were found in the platelet extract eluted from a BB10 or from an IgE immunosorbent column. These findings confirm the similarities between IgE Fc receptors on human eosinophils, platelets, and macrophages, already observed with polyclonal antibodies directed against the B lymphocyte Fc epsilon receptor. They suggest, moreover, that the mAb BB10 can represent a good reagent for further investigations on the structure and the functions of this IgE Fc receptor (Fc epsilon R2).

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Etude in vitro des phenomenes immunologiques dans la schistosomiase humaine et experimentale—I. Etude comparative in vitro de l'activite lethale d'immunserums sur les formes immatures et sur les adultes de Schistosoma mansoni

Cited by 45 publications

References 9 publications

Effector Mechanisms of Immunity to Schistosomes and Their Regulation

Effector Mechanisms of Immunity to Schistosomes and Their Regulation

T-Cell Responsiveness towards Various Synthetic Peptides of the P28 Antigen in Rat and Mouse Models during <i>Schistosoma mansoni</i> Infection

Functional study of a monoclonal antibody to IgE Fc receptor (Fc epsilon R2) of eosinophils, platelets, and macrophages.

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