Etude épidémiologique sur la fièvre catarrhale du mouton chez les ovins, bovins et différentes espèces d'animaux sauvages en Côte d'Ivoire

Formenty, Pierre; Doménech, Joseph; Lauginie, Francis; Ouattara, Mamadou; Diawara, Safere; Jp, Raath; Grobler, Douw; Leforban, Yves; Angba, Assy

doi:10.20506/rst.13.3.798

Cited by 6 publications

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Antelope Diseases – the Good, the Bad and the Ugly

Kock

Chardonnet²,

Risley

2016

Antelope Conservation

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The identity of the cells that form the periosteum during development is controversial with current dogma suggesting these are derived from a Sox9-positive progenitor. Herein, we characterize a newly created Prrx1eGFP reporter transgenic mouse line during limb formation and postnatally. Interestingly, in the embryo Prrx1eGFP-labeled cells become restricted around the Sox9-positive cartilage anlage without themselves becoming Sox9-positive. In the adult, the Prrx1eGFP transgene live labels a subpopulation of cells within the periosteum that are enriched at specific sites, and this population is diminished in aged mice. The green fluorescent protein (GFP)-labeled subpopulation can be isolated using fluorescence-activated cell sorting (FACS) and represents approximately 8% of all isolated periosteal cells. The GFP-labeled subpopulation is significantly more osteogenic than unlabeled, GFP-negative periosteal cells. In addition, the osteogenic and chondrogenic capacity of periosteal cells in vitro can be extended with the addition of fibroblast growth factor (FGF) to the expansion media. We provide evidence to suggest that osteoblasts contributing to cortical bone formation in the embryo originate from Prrx1eGFP-positive cells within the perichondrium, which possibly piggyback on invading vascular cells and secrete new bone matrix. In summary, the Prrx1eGFP mouse is a powerful tool to visualize and isolate periosteal cells and to quantify their properties in the embryo and adult.

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Antelope Diseases – the Good, the Bad and the Ugly

Kock

Chardonnet²,

Risley

2016

Antelope Conservation

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Environmental and host factors affecting seroprevalence of bluetongue virus infections of sheep

Noaman

Arzani

2016

Comp Clin Pathol

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Bluetongue epidemiology in wild ruminants from Southern Spain

et al. 2008

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Serum samples from 210 wild ruminants collected between 2006 and 2007 in southern Spain were tested for antibodies against bluetongue virus (BTV) by means of a competitive ELISA assay. Eighty-seven of the 210 wild ruminants analysed (41%) showed antibodies against BTV. Statistically significant differences were found in the seroprevalence among species: 66% (65 of 98) for red deer (Cervus elaphus), 50% (ten of 20) for fallow deer (Dama dama), 33% (three of nine) for mouflon (Ovis aries musimon) and 11% (nine of 83) for Spanish ibex (Capra pyrenaica). Overall, the sites where seropositive wild ruminants were found coincide with the areas where BTV had been detected in livestock, but in eastern Sierra Morena, the virus circulated in wild ruminants, although it had not been detected in domestic ruminants in the same areas. Wild ruminants over 1-year of age (sub-adults and adults) had significantly higher seroprevalences than juvenile animals. Statistically significant differences were also observed between BTV seroprevalence and management (free-ranging vs. captivity) with higher prevalence in freeranging animals. The high seroprevalences obtained suggest that BTV is widespread in wild ruminants in southern Spain. This factor could have an important influence on the evolution of the infection in domestic livestock and indicates the need to include wild ruminant species in BTV surveillance or control programs.

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Etude épidémiologique sur la fièvre catarrhale du mouton chez les ovins, bovins et différentes espèces d'animaux sauvages en Côte d'Ivoire

Cited by 6 publications

References 0 publications

Antelope Diseases – the Good, the Bad and the Ugly

Antelope Diseases – the Good, the Bad and the Ugly

Environmental and host factors affecting seroprevalence of bluetongue virus infections of sheep

Bluetongue epidemiology in wild ruminants from Southern Spain

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