“…Over the years TMAO’s dipole moment has been measured and calculated by many different methods. ,,,,− The N–O bond length is also of interest in characterizing the physical properties and behavior of TMAO. , Researchers are also interested in the physical properties of TMAO in the biological setting. Many spectroscopic studies, including infrared, ,,,− Raman, ,,,− NMR, , UV–vis absorption, circular dichroism, − and gas electron diffraction have been employed over the years to describe TMAO’s molecular properties and help explain the effects of TMAO on hydrogen-bonded water networks and also biological environments in general.…”