Etude de la teneur en hyoscyamine et scopolamine d'une population sauvage de<i>Datura stramonium</i>L. en Algérie

Houmani, Zahia; Cosson, L.; Corbineau, Françoise; Côme, D.

doi:10.1080/12538078.1994.10515135

Cited by 7 publications

(3 citation statements)

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“…In the spontaneous whole plants of Datura species the mean hyoscyamine contents are between 0.2 -0.8 mg/g D.W. 1 12 , each root line obtained following transformation by A. rhizogenes can be considered a different line from all others on a genetic and biochemical level. Thus, in the production programs of alkaloids via in vitro plant culture technics, the optimization of the transformation by the A. rhizogenes is a crucial and unavoidable step for obtaining a large number of HRs allowing the selection of efficient lines.…”

Section: Discussionmentioning

confidence: 99%

“…This last one is a tropane alkaloid used in medicine as useful natural product. 1,2 In vitro plant cultures drawn an interest and cost-effective alternative to classical approaches to plant secondary metabolite production. They constitute sustainable and eco-friendly system to obtain complex chemical structures biosynthesized by concerned plant species.…”

Section: Introductionmentioning

confidence: 99%

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Hairy Root Technology: Effect of Etiolation on Datura sp. Transgenic Root Induction and Hyoscyamine Production

Harfi¹,

Khelifi²

2019

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Introduction: Hyoscyamine, because of its medicinal properties, is an important tropane alkaloid. In order to implement a biotechnological process for its production, hairy roots of Datura species resulting from genetic transformation by Agrobacterium rhizogenes A4 strain have been the subject of this work. In the in vitro alkaloids production programs, optimization of the transformation is a crucial step for obtaining a large number of root lines allowing the selection of efficient lines. Methods: The conditions for hairy roots induction were explored by studying the effect of Datura sp. in vitro seedling etiolation on the genetic transformation. The first step was the establishment of Datura in vitro plantlet cultures followed by the hairy roots induction with A4 strain of A. rhizogenes. The confirmation of the genetic transformation was performed by polymerase chain reaction (PCR) analysis of rolB gene in the roots. After hyoscyamine extraction, it was analyzed (quantitatively and qualitatively) by gas chromatography. Results: 343 root lines were obtained in total, 96 root lines from non-etiolated Datura in vitro seedlings and 247 root lines from etiolated in vitro plantlets. After the selection of six transgenic root lines, tow root lines from each species (D. tramonium, D. tatula and D. innoxia), one from non-etiolated in vitro plantlet and one from etiolated in vitro plantlet, the most hyoscyamine content was 8.43 mg/g D.W. obtained with D. tatula etiolated in vitro seedling. Conclusion: The etiolated in vitro plantlets seem more favorable to hairy roots induction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hairy Root Technology: Effect of Etiolation on Datura sp. Transgenic Root Induction and Hyoscyamine Production

Harfi¹,

Khelifi²

2019

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“…These plants have also played a significant role in traditional medicine and have been linked to practices involving sorcery, witchcraft, and magico-religious rituals. It is important to note that both of these plants contain the active hallucinogenic compounds atropine and scopolamine [277]. Tropane alkaloids are important natural products that are abundantly present in the Solanaceae family and include the anticholinergic drugs atropine, hyoscyamine, and scopolamine (Figure 16) [278].…”

Section: Toxicological Evidencementioning

confidence: 99%

Therapeutics to Treat Psychiatric and Neurological Disorders: A Promising Perspective from Algerian Traditional Medicine

Larit,

León

2023

Plants

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Ancient people sought out drugs in nature to prevent, cure, and treat their diseases, including mental illnesses. Plants were their primary source for meeting their healthcare needs. In Algeria, folk medicine remains a fundamental part of the local intangible knowledge. This study aims to conduct a comprehensive ethnomedicinal investigation and documentation of medicinal plants and the different plant formulations traditionally used in Algeria for the treatment of pain, psychiatric, and neurological disorders. It also intends to improve the current knowledge of Algerian folk medicine. Several scientific databases were used to accomplish this work. Based on this investigation, we identified 82 plant species belonging to 69 genera and spanning 38 distinct botanical families used as remedies to treat various psychological and neurological conditions. Their traditional uses and methods of preparation, along with their phytochemical composition, main bioactive constituents, and toxicity were noted. Therefore, this review provides a new resource of information on Algerian medicinal plants used in the treatment and management of neurological and psychological diseases, which can be useful not only for the documentation and conservation of traditional knowledge, but also for conducting future phytochemical and pharmacological studies.

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Approaches for Modeling and Optimization of the Secondary Metabolite Production by Plant Biotechnology Methods

Amdoun¹,

Benyoussef²,

Benamghar³

et al. 2020

Reference Series in Phytochemistry

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Etude de la teneur en hyoscyamine et scopolamine d'une population sauvage deDatura stramoniumL. en Algérie

Cited by 7 publications

References 3 publications

Hairy Root Technology: Effect of Etiolation on Datura sp. Transgenic Root Induction and Hyoscyamine Production

Hairy Root Technology: Effect of Etiolation on Datura sp. Transgenic Root Induction and Hyoscyamine Production

Therapeutics to Treat Psychiatric and Neurological Disorders: A Promising Perspective from Algerian Traditional Medicine

Approaches for Modeling and Optimization of the Secondary Metabolite Production by Plant Biotechnology Methods

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