Etoxazole resistance in predatory mite Phytoseiulus persimilis A.-H. (Acari: Phytoseiidae): Cross-resistance, inheritance and biochemical resistance mechanisms

Salman, Sibel Yorulmaz; Aydınlı, Fatma Esen; Ay, Recep

doi:10.1016/j.pestbp.2014.12.014

Cited by 16 publications

(5 citation statements)

References 44 publications

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“…Previous studies have been suggested that detoxification enzymes play roles in clofentezine resistance in T. urticae (Ay & Kara, 2011) and etoxazole resistance in Ph. Persimilis (Yorulmaz Salman et al , 2015). Cytochrome P450s constitute one of the largest and oldest gene superfamilies in all living organisms (Liu & Zhu, 2011; Feyereisen, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…However, these methods are not appropriate in the case of MGIs since MGIs are not toxic to deutonymph and adult stages of T. urticae (Dekeyser, 2005; Nauen & Smagghe, 2006). In the past, various types of bioassays have been employed to test the activities of MGIs (Aveyard et al , 1986; Knight et al , 1990; Rathman et al , 1990; Marcic, 2003; Yorulmaz Salman et al , 2015). Most of these bioassay methods involve direct application of the MGI to eggs (Knight et al , 1990; Uesugi et al , 2002; Demaeght et al , 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies reported that a single non-synonymous mutation (I1017F) in the non-catalytic domain of CHS1 confers resistance to clofentezine, etoxazole, and hexythiazox in etoxazole and hexythiazox-resistant T. urticae populations (Van Leeuwen et al , 2012; Demaeght et al , 2014). Biochemical enzyme activity assays suggest that detoxification enzymes such as carboxylesterases (CoEs), glutathione S-transferases (GSTs), or cytochrome P450s may also contribute to resistance in a clofentezine resistant T. urticae strain and a etoxazole resistant Phytoseiulus persimilis (Acari: Phytoseiidae) strain (Ay & Kara 2011; Yorulmaz Salman et al , 2015).…”

Section: Introductionmentioning

confidence: 99%

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Mechanisms of resistance to three mite growth inhibitors ofTetranychus urticaein hops

Adesanya

Morales

Walsh

et al. 2017

Bull. Entomol. Res.

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Mite growth inhibitors (MGIs), such as etoxazole and hexythiazox, are valuable IPM tools for Tetranychus urticae control in hops due to their unique mode of action and selectivity. Hence, it is necessary to standardize bioassay methods to evaluate the efficacy of MGIs, monitor resistance, and identify mechanisms underlying MGI resistance in the field. Here, we developed a three-tiered approach for evaluating ovicidal toxicity of MGIs to T. urticae, which simulated different MGI exposure scenarios in the field. The most effective bioassay method was direct exposure of T. urticae eggs to MGIs. With this method, four field-collected T. urticae populations showed low-to-moderate resistance to MGIs. Cross-resistance among MGIs and from MGIs to bifenazate and bifenthrin was detected. Besides target site insensitivity, enhanced cytochrome P450 and esterase activities also contribute to the MGI resistance in hop yard-collected T. urticae populations. Low-to-moderate MGI resistance in T. urticae populations may be mediated by multiple mechanisms. Positive selection pressure on the I1017F mutation is moderate in field-collected T. urticae populations. Further studies are required to identify metabolic detoxification genes that confer resistance to MGIs for precise resistance monitoring.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanisms of resistance to three mite growth inhibitors ofTetranychus urticaein hops

Adesanya

Morales

Walsh

et al. 2017

Bull. Entomol. Res.

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“…P450s are known to be related to cyflumetofen resistance [ 33 ]. Additionally, P450s are known to be related to pyridaben and etoxazole resistance, so the ER and PR strains showed cross resistance to cyflumetofen [ 33 , 34 ]. Fluxametamide has a similar mode of action to diamide.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility to Acaricides and the Frequencies of Point Mutations in Etoxazole- and Pyridaben-Resistant Strains and Field Populations of the Two-Spotted Spider Mite, Tetranychus urticae (Acari: Tetranychidae)

Koo

Choi

Shin

et al. 2021

Insects

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The two-spotted spider mite Tetranychus urticae Koch is a major agricultural pest worldwide and is known to rapidly develop resistance to pesticides. In the present study, we explored a field strain that was collected in 2000 and 2003 and has been exhibiting resistance to etoxazole and pyridaben over the last 16 years. The resistance ratios of the etoxazole- and pyridaben-resistant strains (ER and PR) to etoxazole or pyridaben were more than 5,000,000- and 4109.6-fold higher than that of the susceptible strain, respectively. All field-collected populations showed resistance to etoxazole and pyridaben. The ER and PR strains showed cross-resistance to several acaricides. Both I1017F and H92R point mutations were detected in 7 out of 8 field groups. Spirodiclofen and spiromesifen resulted in more than 77.5% mortality in the 8 field groups. In addition, the genotype frequency of the I1017F point mutation was 100.0% in the ER strain, and that of the H92R point mutation was 97.0% in the PR strain. All of the field populations were found to have a high frequency of I1017F. These results suggest that the observation of resistance patterns will help in designing a sustainable IPM program for T. urticae.

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“…To the best of our knowledge, etoxazole resistance mechanisms other than the mutation in CHS1 in T. urticae, whereas the resistance in a phytoseiid mite Phytoseiulus persimilis Athias-Henriot was suggested to have a relevance to the activities of detoxification enzymes by Salman et al [30]. Application of this method to such metabolic resistance system may mostly be difficult, and the application to the detection of SNPs is also limited by the availability of restriction enzyme as well as PCR-RFLP.…”

Section: Discussionmentioning

confidence: 99%

Combination of restriction endonuclease digestion with the ΔΔCt method in real-time PCR to monitor etoxazole resistance allele frequency in the two-spotted spider mite

Osakabe

Imamura

Nakano

et al. 2017

Pesticide Biochemistry and Physiology

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Monitoring resistance allele frequency at the early stage of resistance development is important for the successful acaricide resistance management. Etoxazole is a mite growth inhibitor to which resistance is conferred by an amino acid substitution in the chitin synthase 1 (CHS1; I1017F) in T. urticae. If the susceptible allele can be specifically digested by restriction endonuclease, the ΔΔCt method using real-time PCR for genomic DNA (RED-ΔΔCt method) may be available for monitoring the resistance allele frequency. We tested whether the etoxazole resistance allele frequency in a pooled sample was accurately measured by the RED-ΔΔCt method and validated whether the resistance variant frequency was correlated with etoxazole resistance phenotype in a bioassay. Finally, we performed a pilot test using field populations. Strong linearity of the measures by the RED-ΔΔCt method with practical resistance allele frequencies; resistance allele frequency in the range between 0.5% to at least 0.75% was strictly represented. The strong linear relationship between hatchability of haploid male eggs after the etoxazole treatments (phenotype) and resistance allele frequencies in their mothers provided direct evidence that I1017F is a primary resistance factor to etoxazole in the strains used for experiments. The pilot test revealed a significant correlation between egg hatchability (including both diploid female eggs and haploid male eggs) and estimators in field populations. Consequently, we concluded that the RED-ΔΔCt method is a powerful tool for monitoring a resistance allele in a pooled sample.

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Etoxazole resistance in predatory mite Phytoseiulus persimilis A.-H. (Acari: Phytoseiidae): Cross-resistance, inheritance and biochemical resistance mechanisms

Cited by 16 publications

References 44 publications

Mechanisms of resistance to three mite growth inhibitors ofTetranychus urticaein hops

Mechanisms of resistance to three mite growth inhibitors ofTetranychus urticaein hops

Susceptibility to Acaricides and the Frequencies of Point Mutations in Etoxazole- and Pyridaben-Resistant Strains and Field Populations of the Two-Spotted Spider Mite, Tetranychus urticae (Acari: Tetranychidae)

Combination of restriction endonuclease digestion with the ΔΔCt method in real-time PCR to monitor etoxazole resistance allele frequency in the two-spotted spider mite

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