Etiology of Sweet Potato Chlorotic Dwarf Disease in Argentina

Feo, L. Di; Nomé, S. F.; Biderbost, E.; Fuentes, Segundo; Salazar, Luis

doi:10.1094/pdis.2000.84.1.35

Cited by 42 publications

(17 citation statements)

References 22 publications

(24 reference statements)

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“…The apparent synergistic effect of SPFMV and SPCSV is now well documented (Gutierrez et al 1999). Nevertheless, viral synergism is not exclusively restricted to SPFMV and SPCSV, as other virus interactions have been reported (Di Feo et al 2000; Salazar and Fuentes 2000; Clark and Hoy 2006). The symptomatology seems to be different depending on the virus complex and is difficult to be distinguished by inexperienced observers.…”

Section: Discussionmentioning

confidence: 90%

“…Sweetpotato viral disease (SPVD), caused by the synergistic interaction of sweetpotato feathery mottle virus and sweetpotato chlorotic stunt virus, reduces yield by up to 98 % (Mwanga et al 2002; Clark et al 2012). Besides this, chlorotic dwarf, Camote Kulot and some other complex infections exist (Di Feo et al 2000; Salazar and Fuentes 2000). Virtually all sweetpotatoes grown from non-virus-tested materials revealed the presence of one or more viruses in them (Moyer and Salazar 1989).…”

Section: Introductionmentioning

confidence: 99%

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Elimination and detection of viruses in meristem-derived plantlets of sweetpotato as a low-cost option toward commercialization

et al. 2012

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Viral diseases affecting sweetpotato are the most devastating and cause up to 98 % yield loss. In this paper, we report, meristem culture, graft transmission and virus indexing for management of viral pathogens in seven elite sweetpotato cultivars. Plantlets were developed in vitro from the apical meristematic dome with one to two leaf primordia. Mericlones were grafted on virus-sensitive indicator plant Ipomoea setosa and no viral disease symptoms were seen on I. setosa leaves in most cases. This indicates that no viruses translocated from meristem-derived scions to the virus-sensitive root stock. On the other hand, most of the non-tested traditional planting material induced distinct disease symptoms upon grafting, which revealed the presence of one or more viruses in it. About 85 % of mericlones recovered from 0.3–0.5 mm size meristem were tested as virus free, whereas it is difficult to culture meristems smaller than 0.3 mm due to dissection damage and too small a size. Virus-tested mericlones were further micropropagated and transferred to the field. Only few plants were found to be diseased in the R1 field trial. Root yield in the R2 generation was increased significantly when compared with non-tested control plants. During field exposure, only a low percentage of healthy plants were found infected with viruses when managed in a net house. This implies that viral vectors were present during the growing season and reinfection could be effectively reduced by net house management. We concluded that this low-cost technique of producing virus-tested planting material would significantly boost the yield through efficient removal of yield-reducing pathogens.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Elimination and detection of viruses in meristem-derived plantlets of sweetpotato as a low-cost option toward commercialization

et al. 2012

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“…However, no effect was observed when sap from white clover was used, suggesting that inhibitor levels vary greatly even within cultivars of the same species. However, the inherent difficulty in isolating and detecting SPFMV as well as other viruses directly from sweetpotato is reflected in the fact that the majority of studies used indicator plants such as I. setosa, I. nil, and others for the indirect isolation of these viruses (6,8,14,17,24). Even though, at 3 weeks after inoculation, the titers of SPFMV were not different among the three hosts, the titer levels of potyviruses in I. setosa and I. nil were clearly and consistently above the threshold of detection; whereas, in sweetpotato plants, they often were near or below the threshold.…”

Section: Discussionmentioning

confidence: 99%

“…The second step involved the actual testing of the selected primer/probe sets against templates of either total RNA or DNA extracted from leaves of sweetpotato plants graft inoculated separately with SPFMV-RC, IVMV, SPVG, SPCSV (isolate BWFT-3), and the United States strain of SPLCV, SPLCV-US (isolate SWFT-1). In addition, the SPFMV primer/MGB probe set was tested against preparations of SPFMV-RC and SPFMV-C (common strain of SPFMV) particles purified following the procedures of DiFeo et al (8) and adjusted to a concentration of 25 µg/ml based on absorption at 260 and 280 nm. Total RNA and DNA extracts from virus-tested plants were included in each test.…”

Section: Methodsmentioning

confidence: 99%

Real-Time PCR Assays for Detection and Quantification of Sweetpotato Viruses

Kokkinos

Clark

2006

Plant Disease

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Viral diseases, especially those caused by mixed infections, are among the economically most important diseases of sweetpotato. The difficulties inherent in detecting, quantifying, and isolating viruses directly from sweetpotato have impeded progress in sweetpotato virus research. Real-time polymerase chain reaction (PCR) assays were developed for the detection and relative quantification in singleplex reactions of the potyviruses Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG), and Ipomoea vein mosaic virus (IVMV); the crinivirus Sweet potato chlorotic stunt virus (SPCSV); and the begomovirus Sweet potato leaf curl virus(SPLCV) directly from infected sweetpotato plants. There was no significant effect from potential inhibitors in total nucleic acid extracts from sweetpotato leaves on the performance of the real-time PCR assays. Virus titers of SPFMV, IVMV, and SPVG were quantified using real-time PCR and found to be lower in singly infected sweetpotato plants compared with singly infected Brazilian morning-glory (Ipomoea setosa Ker.) and I. nil cv. Scarlet O'Hara plants. Real-time PCR was a more efficient detection method for SPLCV than conventional PCR assay.

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“…It is a required component for the manifestation of several severe viral diseases caused by coinfection with other viruses (9,13,23). For example, sweet potato virus disease is caused by coinfection with SPCSV and Sweet potato feathery mottle virus (genus Potyvirus) and is the major disease of sweet potato in Africa and perhaps worldwide (8).…”

mentioning

confidence: 99%

Complete Genome Sequence and Analyses of the Subgenomic RNAs of Sweet Potato Chlorotic Stunt Virus Reveal Several New Features for the Genus Crinivirus

Kreuze

Savenkov

Valkonen

2002

J Virol

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The complete nucleotide sequences of genomic RNA1 (9,407 nucleotides [nt]) and RNA2 (8,223 nt) of Sweet potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) were determined, revealing that SPCSV possesses the second largest identified positive-strand single-stranded RNA genome among plant viruses after Citrus tristeza virus. RNA1 contains two overlapping open reading frames (ORFs) that encode the replication module, consisting of the putative papain-like cysteine proteinase, methyltransferase, helicase, and polymerase domains. RNA2 contains the Closteroviridae hallmark gene array represented by a heat shock protein homologue (Hsp70h), a protein of 50 to 60 kDa depending on the virus, the major coat protein, and a divergent copy of the coat protein. This grouping resembles the genome organization of Lettuce infectious yellows virus (LIYV), the only other crinivirus for which the whole genomic sequence is available. However, in striking contrast to LIYV, the two genomic RNAs of SPCSV contained nearly identical 208-nt-long 3 terminal sequences, and the ORF for a putative small hydrophobic protein present in LIYV RNA2 was found at a novel position in SPCSV RNA1. Furthermore, unlike any other plant or animal virus, SPCSV carried an ORF for a putative RNase III-like protein (ORF2 on RNA1). Several subgenomic RNAs (sgRNAs) were detected in SPCSV-infected plants, indicating that the sgRNAs formed from RNA1 accumulated earlier in infection than those of RNA2. The 5 ends of seven sgRNAs were cloned and sequenced by an approach that provided compelling evidence that the sgRNAs are capped in infected plants, a novel finding for members of the Closteroviridae.

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Etiology of Sweet Potato Chlorotic Dwarf Disease in Argentina

Cited by 42 publications

References 22 publications

Elimination and detection of viruses in meristem-derived plantlets of sweetpotato as a low-cost option toward commercialization

Elimination and detection of viruses in meristem-derived plantlets of sweetpotato as a low-cost option toward commercialization

Real-Time PCR Assays for Detection and Quantification of Sweetpotato Viruses

Complete Genome Sequence and Analyses of the Subgenomic RNAs of Sweet Potato Chlorotic Stunt Virus Reveal Several New Features for the Genus Crinivirus

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