Etiology and antimicrobial susceptibility profiles of anaerobic bacteria isolated from clinical samples in a university hospital in Madrid, Spain

López-Pintor, José María; García‐Fernández, Sergio; Ponce-Alonso, Manuel; Sánchez-Díaz, Ana Maria; Ruíz-Garbajosa, Patricia; Morosini, Marı́a-Isabel

doi:10.1016/j.anaerobe.2021.102446

Cited by 9 publications

(27 citation statements)

References 29 publications

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“…Increasing high levels of penicillin resistance have been observed for Bacteroides isolates over the years. More speci cally, the penicillin resistance rate of Bacteroides isolates was reported as 80% in 2010 [27], 97.1% in 2015 [28], 94% in 2019 [23], and 88.5% in 2021 [25]. In agreement with these studies, in the present study, a penicillin resistance rate of 100% was determined for the seven tested Bacteroides isolates.…”

Section: Discussionsupporting

confidence: 88%

Anaerobic bacteria from bloodstream infections: Comparison of three methods for identification and detection of the antibacterial susceptibilities of recovered isolates

Orak,

Karakaya,

Satıcıoğlu

et al. 2024

Preprint

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This study was aimed at the comparison of three different methods used for the identification of anaerobic bacteria isolated from blood cultures and the determination of the antibacterial susceptibilities of the isolates. The study material comprised of 5282 blood samples. The samples were incubated in a BacT/ALERT system. The species identification of the isolates was performed using BBL Crystal Anaerobe system, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and 16S rRNA gene sequence analysis. Antibacterial susceptibility testing was performed using the disk diffusion method with benzylpenicillin, clindamycin, piperacillin-tazobactam, meropenem, and metronidazole disks. In the BacT/ALERT system, forty-five anaerobic bacterial isolates were recovered from 39 (0.74%) of the samples that showed growth signs in blood culture bottles. The BBL Crystal Anaerobe system and 16S rRNA gene sequence analyses enabled the genus and species identification of all 45 isolates (100%), whereas with MALDI-TOF MS, only 37 (82.2%) of the isolates were able to be identified. The compatibility of the three tests for the identification of the isolates at genus and species level was 62.2% (28/45) and 37.8% (17/45), respectively. Antibacterial resistance rates of the isolates to piperacillin/tazobactam, clindamycin, benzylpenicillin, meropenem, and metronidazole were detected as 100%, 73.8%, 40%, 9.8%, and 5.5%, respectively. MALDI-TOF MS showed a higher level of compatibility with 16S rRNA gene sequence analyses, compared to the BBL Crystal Anaerobe system. The high rates of susceptibility to meropenem and metronidazole suggested that these antibiotics could be safely used for the empirical treatment of anaerobic bacterial infections.

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Section: Discussionsupporting

confidence: 88%

Anaerobic bacteria from bloodstream infections: Comparison of three methods for identification and detection of the antibacterial susceptibilities of recovered isolates

Orak,

Karakaya,

Satıcıoğlu

et al. 2024

Preprint

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“…Veillonella spp. have high rates of penicillin resistance, ranging from 29 to 55%, except in Korea, where Buyn et al reported 100% of resistance (n = 11), in contrast to Ali et al, who reported no resistant strains in Ireland (n = 9) [17,19,21,22,27,28]. Among Veillonella spp., high levels of resistance to TZP (MIC ≥ 128 mg/L) were observed [17,21,22].…”

Section: β-Lactamsmentioning

confidence: 96%

“…A decrease in susceptibility to meropenem among the B. fragilis group was also reported in Japan between 2010 and 2018–2019 (98% to 90%) [ 16 ]. In recent studies, AMC resistance ranges from 2 to 9% in B. fragilis, except in Spain, where higher rates were reported (29%) [ 15 , 17 , 18 , 19 , 20 ]. PTZ resistance remains lower, with a resistance rate varying between 1 and 3%, while the resistance rate reaches 5% in Korea and Greece [ 17 , 21 , 22 , 23 ].…”

Section: β-Lactamsmentioning

confidence: 99%

“…Higher AMC and PTZ resistance rates were reported in the B. fragilis group excluding B. fragilis , especially in B. thetaiotaomicron and Phocaeicola vulgatus [ 17 , 18 , 23 ]. In B. fragilis , the rate of resistance to carbapenems ranges from 0 to 5% for imipenem and from 2 to 5% for meropenem [ 15 , 17 , 18 , 19 , 20 , 21 , 23 , 24 ].…”

Section: β-Lactamsmentioning

confidence: 99%

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Recent Trends in Antimicrobial Resistance among Anaerobic Clinical Isolates

et al. 2023

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Anaerobic bacteria are normal inhabitants of the human commensal microbiota and play an important role in various human infections. Tedious and time-consuming, antibiotic susceptibility testing is not routinely performed in all clinical microbiology laboratories, despite the increase in antibiotic resistance among clinically relevant anaerobes since the 1990s. β-lactam and metronidazole are the key molecules in the management of anaerobic infections, to the detriment of clindamycin. β-lactam resistance is usually mediated by the production of β-lactamases. Metronidazole resistance remains uncommon, complex, and not fully elucidated, while metronidazole inactivation appears to be a key mechanism. The use of clindamycin, a broad-spectrum anti-anaerobic agent, is becoming problematic due to the increase in resistance rate in all anaerobic bacteria, mainly mediated by Erm-type rRNA methylases. Second-line anti-anaerobes are fluoroquinolones, tetracyclines, chloramphenicol, and linezolid. This review aims to describe the up-to-date evolution of antibiotic resistance, give an overview, and understand the main mechanisms of resistance in a wide range of anaerobes.

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“…They have a broad spectrum of activity with low toxicity and are efficacious across anaerobic genera. However, recent resistance to this has been observed with a prevalence of 60–80% in European countries [ 11 , 12 , 28 ], USA (65%), Canada 63.5% and as high as 91% in Korea [ 8 – 10 ]. In our study, the resistant rates ranged from 58 to 65% in Gram-negative anaerobes and as high as 100% in Gram-positives.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance pattern of anaerobic bacteria causing lower respiratory tract infections

Shariff,

Ramengmawi

2023

BMC Microbiol

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Background Anaerobes are normal flora of the human body. However, they can cause serious infections in humans. Anaerobic bacteria are known to cause respiratory infections like pneumonia and acute exacerbation of chronic lower airway infections. These are often missed due to the complexity of their isolation and identification. Hence, this study aimed to study anaerobes causing respiratory tract infections and determine their antibiotic susceptibility. Materials & methods Clinical specimens such as bronchial aspirates and pleural aspirates collected from patients with respiratory diseases attending Vallabhbhai Patel Chest Institute were processed, the anaerobes isolated were identified, and their susceptibilities to various groups of antimicrobials were studied using standard microbiological methods. Results Three hundred and fourteen patients were included in the study, 154 males and 160 females. Of these 314 patients, 148 (47%) yielded anaerobes in their clinical samples. Seventy patients had more than one type of anaerobic organism. Hence, 235 isolates were recovered belonging to as many as 17 genera. The MIC of seven antibiotics on 154 isolates was tested. The isolates belonged mostly to the genera Bacteroides, Prevotella, Veillonella, and Actinomyces. Variable resistance was observed to most classes of antibiotics by many genera. Conclusions Metronidazole is commonly used against anaerobes, but the study showed that the isolates were 20–30% resistant to the antibiotic. Starting this as an empirical therapy might lead to treatment failure.

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Etiology and antimicrobial susceptibility profiles of anaerobic bacteria isolated from clinical samples in a university hospital in Madrid, Spain

Cited by 9 publications

References 29 publications

Anaerobic bacteria from bloodstream infections: Comparison of three methods for identification and detection of the antibacterial susceptibilities of recovered isolates

Anaerobic bacteria from bloodstream infections: Comparison of three methods for identification and detection of the antibacterial susceptibilities of recovered isolates

Recent Trends in Antimicrobial Resistance among Anaerobic Clinical Isolates

Antimicrobial resistance pattern of anaerobic bacteria causing lower respiratory tract infections

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