Etiologic Relationship of the RI-67 Agent to “Acute Respiratory Disease (ARD)”1

Abstract

As the result of intensive studies of 9ctte respiratory infection in military populations-from 1941 to 1946, an entity termed "acute respiratory disease" was described by the Commission on Acute Respiratory Diseases (1-4). This syndrome, for convenience, was termed "ARD," and this designation will be employed hereafter. ARD is an acute febrile respiratory infection of short duration with both constitutional symptoms and localized respiratory symptoms characterized predominately by cough and hoarseness (1, 2). In addition, irritated throat, nasal obstruction, and chest pain were frequent complaints. Epidemiological investigation (1) as well as human transmission experiments (3, 4) indicated that the incubation period of this infection was five to six days, and that during convalescence there developed an homologous immunity but not heterologous resistance to common cold or primary atypical pneumonia (4). It was postulated as a result of these investigations (1-4) that ARD was an entity distinguishable from other acute respiratory illnesses such as common cold, streptococcal pharyngitis and tonsillitis, and primary atypical pneumonia, and that it was caused by a single filterable agent, probably a virus, or a closely related group of agents. Attempts to isolate an agent in experimental animals and chick embryos were not successful.Although ARD has not been recognized in epidemic form in civilian populations, similar infections such as non-streptococcal pharyngitis and grippe-like illnesses not caused by influenza vi-I

Acute Respiratory Illness in Volunteers Following Intramuscular Administration of Live Adenovirus

“…-DISCUSSION Previous workers have reported the development of acute respiratory illness in human volunteers inoculated nasally with the filtered throat washings from a patient with ARD (4,20,21) and in human beings inoculated by the conjunctival or nasal route with adenoviruses propagated in tissue culture (22,23). The present experiments represent the first successful reproduction of acute respiratory disease in persons given adenovirus by a parenteral route and strongly support the etiological significance of these agents in respiratory illnesses of man.…”

SEQUENTIAL CELLULAR CHANGES PRODUCED BY TYPES 5 AND 7 ADENOVIRUSES IN HELA CELLS AND IN HUMAN AMNIOTIC CELLS

“…Tissue Culture: HeLa Cd/s.--Stock cells of the HeLa strain (6) were cultivated in Eagle's basal medium (7) supplemented with 10 per cent human serum, as previously described (8). For cytological and fluorescein-labelled antibody studies the.cells were grown on coverslip pieces in 16 X 150 ram.…”

Search for Illness Due to Adenovirus Type 4 Among College Dormitory Freshmen.

“…The illness became known as "acute respiratory disease (ARD) ." More recently, the etiology of the disease was ascribed principally to adenovirus type 4, although adenovirus type 7 was shown to be a cause, and adenovirus types 3 and 14 possible causes of such illness (2,3,7,8). With development of methods for detection of serum antibodies, additional tools became available for Reszdts.…”