Etileno glicol na criopreservação de sêmen canino

Soares, Marcio Pereira; Rossi, Carlos Augusto Rigon; Mezzalira, Alceu; Cecim, Marcelo

doi:10.1590/s0103-84782002000400017

Cited by 14 publications

(4 citation statements)

References 9 publications

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“…Vanucchi et al (2002) reported a progressive motility of 62.9% for frozen semen using 5% EG as a cryoprotectant. Soares et al (2002) obtained similar results with semen which was frozen in extenders containing 0.25 or 0.5M of EG. These data showed that T2 preserved better the structural integrity of the plasma membrane than the two other treatments.…”

Section: Discussionsupporting

confidence: 62%

In vitro evaluation of canine spermatozoa cryopreserved in different extenders

Oliveira¹,

Juliani²,

Marques³

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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The efficacy of three extenders, tris-egg yolk-5% ethylene glycol (T1), lactose-egg yolk-5% ethylene glycol (T2) and lactose-egg yolk-5% dimethyl formamide (T3) on preserving the viability of postthawing canine spermatozoa was evaluated. Three ejaculates per dog were obtained of five animals. The semen was packaged in 0.5ml straws and cooled to 4°C for 120min. The straws were frozen 4cm above the nitrogen level for 15min and thawed in water-bath at 37°C for 60sec and at 75°C for 7sec. Progressive motility and vigour were evaluated immediately after thawing (time 0) and at 30, 60, 90 and 120min. Structural and functional integrity of plasma membrane of the spermatozoa were evaluated, respectively, by fluorescent staining probes and hypoosmotic swelling test. Lactose-egg yolk based extenders showed better cryoprotectant capability and dimethyl formamide was an alternative cryoprotectant agent for dog sperm cells.Keywords: dog, semen, cryopreservation, ethylene glycol, dimethyl formamide RESUMO Avaliou-se a eficácia de três diluidores, tris-gema com 5% de etileno glycol (T1), lactose-gema com 5% de etileno glicol (T2) e lactose-gema com 5% de dimetil-formamida (T3) na

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Section: Discussionsupporting

confidence: 62%

In vitro evaluation of canine spermatozoa cryopreserved in different extenders

Oliveira¹,

Juliani²,

Marques³

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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“…Entretanto as sementes crioconservadas, com o uso de crioprotetor (glicerol 50%), apresentaram menores teores de proteína e açúcar solúvel indicando inibição da atividade metabólica, provavelmente, devido ao aumento no teor de água das sementes pelo crioprotetor, capaz de promover danos às membranas celulares e ainda possivelmente à toxicidade do glicerol (Stanwood, 1985). Apesar de o glicerol ser o crioprotetor mais utilizado no congelamento de sêmem de espécies domésticas, seus efeitos deletérios sobre os espermatozóides ocorrem, provavelmente, pelas alterações causadas na membrana plasmática (Soares et al, 2002). Caso seja necessário o uso de crioprotetores para viabilizar o processo de crioconservação, deverá haver um esforço para dimuinuir a toxicidade do crioprotetor utilizando-se diferentes grupos químicos, concentrações e ainda adequando o tempo de exposição.…”

Section: Composição Químicaunclassified

Crioconservação em sementes de cebola

Molina¹,

Tillmann²,

Dode

et al. 2006

Rev. bras. sementes

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RESUMO -O presente trabalho teve como objetivo avaliar os efeitos de diferentes técnicas de crioconservação na qualidade fisiológica, composição química e índice mitótico de sementes de cebola. Sementes de cebola, cultivar Baia Periforme, foram expostas, durante uma hora, a duas técnicas de crioconservação: (a) sem e (b) com crioprotetor (glicerol 50%), seguidas de três métodos de descongelamento: (1) temperatura ambiente de 25°C, durante duas horas; (2) banho-maria a 37°C, durante cinco minutos; (3) microondas em potência de 70W, durante cinco minutos. Antes e após a crioconservação, foram realizadas as seguintes avaliações: teor de água, germinação, vigor (envelhecimento acelerado, primeira contagem de germinação e comprimento de raiz e de parte aérea das plântulas), composição química (teor de açúcar solúvel, proteínas e amido), análise do ciclo celular e índice mitótico. A imersão em nitrogênio líquido, sem o uso de crioprotetor, é uma técnica adequada para a crioconservação de sementes de cebola, não altera a percentagem de germinação e a composição química das sementes, sendo também o tratamento que proporciona maior número de células em divisão celular após o descongelamento das sementes. O tratamento criogênico, com uso de glicerol 50% como crioprotetor, é prejudicial à qualidade fisiológica e altera a composição química de sementes de cebola. O descongelamento das sementes em microondas é mais adequado do que em temperatura ambiente ou banho-maria, porém este último possibilita maior índice mitótico que os demais tratamentos.Termos para indexação: Allium cepa, armazenamento, nitrogênio líquido, qualidade fisiológica. CRYOCONSERVATION IN ONION SEEDSABSTRACT -The present paper aimed to evaluate the effects in the physiological, chemical and cellular quality of cryopreserved seeds through different techniques. Onion seeds of the Baia Periforme cultivar were submitted, for one hour, to two cryopreservation techniques. (a) immersion in N 2 fluid (-196°C) with cryoprotector (glycerol 50%) and (b) immersion in N 2 fluid. After the cryogenization period of time, the seeds were submitted to three thawing methods: (1) room temperature of 25ºC for two hours; (2) water bath (double-boiler) at 37ºC for five minutes; (3) microwave the 70W power for five minutes. Before and after the cryopreservation, the following parameters were assessed: water content, germination, vigour (accelerated aging, first counting, length of root and of the air parts of the plantules), chemical composition (soluble sugar, proteins and starch contents) and analysis of the cell cycle and mitotic index. The immersion in fluid nitrogen, without the use of cryoprotector, is an adequate technique for the cryopreservation of onion seeds that does not alter the germination nor the chemical composition of the seeds, and it was also the treatment that presented the highest number of cells in cell division of all of the melting methods. The cryogenic treatment with the use of glycerol 50% as cryoprotector damaged the physiological quality and altered...

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“…Moreover, a study on the cryopreservation of canine semen can serve as a basis for research on endangered canid species, such as the Brazilian maned wolf (Chrysocyon brachyurus), field fox (Pseudalopex sp.) (Silva, et al, 2000) and the dog do mato (Cerdocyon thous) (Soares, et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Seminal plasma interference in the kinetics and viability of cryopreserved canine sperm

Rigoto¹,

Guaitolini²,

Crespilho³

et al. 2020

RSD

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Most researchers have frozen dog semen using methodologies described for other species. Consequently, these studies have shown that the thawed semen of dogs is of low quality, with conception rates lower than that of other species. Therefore, in this study, we evaluated different freezing protocols for canine semen, using three adult Bulldog Campeiro males, aged 2 to 5 years, with proven fertility. Five semen samples were collected from each animal using the penile bulb digital manipulation method. The collected samples were divided into: group 1, the samples were diluted directly in Botudog® commercial freezing medium (Botupharma Biotecnologia Animal) and group 2, the samples were centrifuged at 600 g for 10 min and the pellet was resuspended in Botudog®, totaling a concentration end of 200 x 106 sperm per ml. The samples were packaged in 0.5 mL straws at a concentration of 100 x 106 viable sperm. The samples remained for 1 h in stabilization at 4 °C and transferred to nitrogen vapor for 20 min, immersed in nitrogen, stored in a cryogenic cylinder, and thawed at 46 °C for 20 s. It was found that the total motility (MT, %), path speed (VAP, μm/s), progressive motility (PM, %), progressive linear speed (VSL; µm/s), curvilinear speed (VCL; µm/s), linearity (LIN, %), percentage of fast sperm (RAP, %), and plasma and acrosomal membrane integrity were higher in group 1, with samples not being centrifuged. These data demonstrate that the canine semen freezing protocol, using the Botudog® diluent, does not recommend the centrifugation of the ejaculate, prior to freezing.

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Etileno glicol na criopreservação de sêmen canino

Cited by 14 publications

References 9 publications

In vitro evaluation of canine spermatozoa cryopreserved in different extenders

In vitro evaluation of canine spermatozoa cryopreserved in different extenders

Crioconservação em sementes de cebola

Seminal plasma interference in the kinetics and viability of cryopreserved canine sperm

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