Ethylenoxid [MAK Value Documentation in German language, 2002]

doi:10.1002/3527600418.mb7521d0034

The MAK‐Collection for Occupational Health and Safety 2012

DOI: 10.1002/3527600418.mb7521d0034

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Ethylenoxid [MAK Value Documentation in German language, 2002]

Abstract: Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 34. Lieferung, Ausgabe 2002 Der Artikel enthält folgende Kapitel: Genotoxizität In vitro In vivo Untersuchungen an somatischen Zellen Exponierte Arbeiter Untersuchungen an Keimzellen … Show more

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Cited by 2 publications

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Hämoglobinaddukte von Ethylenoxid (N‐(2‐Hydroxyethyl)valin), Propylenoxid (N‐(2‐Hydroxypropyl)valin), Acrylnitril (N‐(2‐Cyanoethyl)valin), Acrylamid (N‐(2‐Carbonamidethyl)valin) und Glycidamid (N‐(2‐Hydroxy‐2‐carbonamidethyl)valin) [Biomonitoring Methods in German Language, 2015]

Schettgen

Müller

Ferstl

et al. 2016

The MAK‐Collection for Occupational Health and Safety

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The method permits the simultaneous quantification of the N‐terminal haemoglobin adducts N‐(2‐hydroxyethyl)valine (HEV), N‐(2‐hydroxypropyl)valine (HPV), N‐(2‐cyanoethyl)valine (CEV), N‐(2‐carbonamide ethyl)valine (AAV), and N‐(2‐hydroxy‐2‐carbonamide ethyl)valine (GAV) of the carcinogens ethylene oxide, propylene oxide, acrylonitrile, acrylamide, as well as of glycidamide the metabolite of acrylamide in blood. The adducts which are covalently bound to the N‐terminal valine of the globin chain are cleaved off from the globin chain by a modified Edman degradation. The resulting pentafluorophenyl thiohydantoine derivatives of the valine adducts are extracted with diethyl ether, purified and then subjected to tandem mass spectrometric detection. To protect the vicinal hydroxyl group of the glycidamide adduct, the modified Edman degradation is followed by a second derivatization using sulphuric acetone. For calibration, standards are prepared in non‐smoker pooled globin, adding solutions of dipeptide standards simulating the adduct‐bearing latter two N‐terminal amino acids of the haemoglobin chain. They are processed in the same way as the samples to be analyzed. As internal standards, solutions of isolated globin, which has prior been transformed with the deuterated compounds d3‐acrylo‐nitrile, d 3 ‐acrylamide or d 3 ‐propylene oxide, are used. As internal standard for glycidamide, the d 8 ‐labeled, acetonized pentafluorophenyl thiohydantoine of the glycidamide valine adduct is used.

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Hämoglobinaddukte von Ethylenoxid (N‐(2‐Hydroxyethyl)valin), Propylenoxid (N‐(2‐Hydroxypropyl)valin), Acrylnitril (N‐(2‐Cyanoethyl)valin), Acrylamid (N‐(2‐Carbonamidethyl)valin) und Glycidamid (N‐(2‐Hydroxy‐2‐carbonamidethyl)valin) [Biomonitoring Methods in German Language, 2015]

Schettgen

Müller

Ferstl

et al. 2016

The MAK‐Collection for Occupational Health and Safety

View full text Add to dashboard Cite

show abstract

Haemoglobin adducts of ethylene oxide (N‐(2‐hydroxyethyl)valine), propylene oxide (N‐(2‐hydroxypropyl)valine), acrylonitrile (N‐(2‐cyanoethyl)valine), acrylamide (N‐(2‐carbonamide ethyl)valine) and glycidamide (N‐(2‐hydroxy‐2‐carbonamide ethyl)valine) [Biomonitoring Methods, 2015]

Schettgen¹,

Müller²,

Ferstl³

et al. 2016

The MAK‐Collection for Occupational Health and Safety

View full text Add to dashboard Cite

The method permits the simultaneous quantification of the N‐terminal haemoglobin adducts N‐(2‐hydroxyethyl)valine (HEV), N‐(2‐hydroxypropyl)valine (HPV), N‐(2‐cyanoethyl)valine (CEV), N‐(2‐carbonamide ethyl)valine (AAV), and N‐(2‐hydroxy‐2‐carbonamide ethyl)valine (GAV) of the carcinogens ethylene oxide, propylene oxide, acrylonitrile, acrylamide, as well as of glycidamide the metabolite of acrylamide in blood. The adducts which are covalently bound to the N‐terminal valine of the globin chain are cleaved off from the globin chain by a modified Edman degradation. The resulting pentafluorophenyl thiohydantoine derivatives of the valine adducts are extracted with diethyl ether, purified and then subjected to tandem mass spectrometric detection. To protect the vicinal hydroxyl group of the glycidamide adduct, the modified Edman degradation is followed by a second derivatization using sulphuric acetone. For calibration, standards are prepared in non‐smoker pooled globin, adding solutions of dipeptide standards simulating the adduct‐bearing latter two N‐terminal amino acids of the haemoglobin chain. They are processed in the same way as the samples to be analyzed. As internal standards, solutions of isolated globin, which has prior been transformed with the deuterated compounds d 3 ‐acrylo‐nitrile, d 3 ‐acrylamide or d 3 ‐propylene oxide, are used. As internal standard for glycidamide, the d 8 ‐labeled, acetonized pentafluorophenyl thiohydantoine of the glycidamide valine adduct is used.

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Ethylenoxid [MAK Value Documentation in German language, 2002]

Abstract: Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 34. Lieferung, Ausgabe 2002 Der Artikel enthält folgende Kapitel: Genotoxizität In vitro In vivo Untersuchungen an somatischen Zellen Exponierte Arbeiter Untersuchungen an Keimzellen … Show more

Cited by 2 publications

References 57 publications

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