Ethylene suppresses tomato (<i>Solanum lycopersicum</i>) fruit set through modification of gibberellin metabolism

Shinozaki, Yoshihito; Hao, Shuhei; Kojima, Mikiko; Sakakibara, Hitoshi; Ozeki-Iida, Yuko; Zheng, Yi; Fei, Zhangjun; Zhong, Silin; Giovannoni, James J.; Rose, Jocelyn K. C.; Okabe, Yoshihiro; Heta, Yumi; Ezura, Hiroshi; Ariizumi, Tohru

doi:10.1111/tpj.12882

Cited by 127 publications

(111 citation statements)

References 62 publications

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“…The samples were harvested and frozen in liquid nitrogen at the indicated time points. The GAs were quantified with ultra‐high performance liquid chromatography (UHPLC)‐electrospray interface (ESI) and quadrupole‐orbitrap mass spectrometry (UHPLC/Q‐Exactive; Thermo Scientific) using an ODS column (AQUITY UPLC BEH C18 1.7 μm, 2.1 × 100 mm, Waters, http://www.waters.com/) as described previously (Kojima et al ., ; Shinozaki et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Suppression of DELLA signaling induces procambial cell formation in culture

et al. 2018

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The post-embryonic growth of plants requires the activities of apical meristems and lateral meristems. In the meristems, self-proliferation and differentiation of stem cells is tightly modulated by plant hormone signaling networks and specific transcription factors. Despite extensive studies on stem cell maintenance in plants, the mechanism by which stem cells are initially established is largely unknown. Vascular stem cells consisting of procambial/cambial cells give rise to xylem and phloem cells. In this study, we analyzed the establishment of procambial cells using the in vitro culture system VISUAL, in which mesophyll cells rapidly differentiate into xylem tracheary elements and phloem sieve elements via procambial cells. We found that procambial cell formation in VISUAL is initiated by light, which can be replaced by application of gibberellin (GA). Gibberellin was able to promote procambial cell formation through degradation of DELLA, whereas light did not elevate the endogenous GA content. Indeed, light in combination with bikinin reduced the accumulation of DELLA protein in VISUAL. Consistently, overexpression of a constitutively active DELLA protein repressed vascular cell differentiation even under light. These combined results suggest that DELLA signaling suppresses procambial cell formation during vascular development in VISUAL.

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Section: Methodsmentioning

confidence: 97%

Suppression of DELLA signaling induces procambial cell formation in culture

et al. 2018

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“…GC‐MS analysis was performed using a previously described system (Avin‐Wittenberg et al ., ), comprising a CTC CombiPAL autosampler (http://www.ctc.ch/), an Agilent 6890N gas chromatograph (http://agilent.com./) and a LECO Pegasus III TOF‐MS (http://www.leco.com/). LC‐MS analysis was performed as described previously (Kojima et al ., ; Shinozaki et al ., ) using an LC‐MS system (AQUITY UPLC System/XEVO‐TQS; Waters, http://www.waters.com/) with an ODS column (AQUITY UPLC HSS T3, 1.8 μm, 2.1 × 100 mm; Waters) for cytokinins and an ultra‐high‐performance liquid chromatography‐Q‐Exactive™ system (Thermo Scientific, https://www.thermofisher.com/) with an ODS column (AQUITY UPLC BEH C18, 1.7 μm, 2.1 × 100 mm; Waters) for other metabolites. The Roche Yellow line Maltose/Sucrose/D‐Glucose assay kit (r‐Biopharm, http://www.r-biopharm.com/) was also employed for the glucose quantification.…”

Section: Methodsmentioning

confidence: 99%

Temporal and spatial changes in gene expression, metabolite accumulation and phytohormone content in rice seedlings grown under drought stress conditions

et al. 2017

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In order to analyze the molecular mechanisms underlying the responses of plants to different levels of drought stress, we developed a soil matric potential (SMP)-based irrigation system that precisely controls soil moisture. Using this system, rice seedlings were grown under three different drought levels, denoted Md1, Md2 and Md3, with SMP values set to -9.8, -31.0 and -309.9 kPa, respectively. Although the Md1 treatment did not alter the visible phenotype, the Md2 treatment caused stomatal closure and shoot growth retardation (SGR). The Md3 treatment markedly induced SGR, without inhibition of photosynthesis. More severe drought (Sds) treatment, under which irrigation was terminated, resulted in the wilting of leaves and inhibition of photosynthesis. Metabolome analysis revealed the accumulation of primary sugars under Md3 and Sds and of most amino acids under Sds. The starch content was increased under Md3 and decreased under Sds. Transcriptome data showed that the expression profiles of associated genes supported the observed changes in photosynthesis and metabolites, suggesting that the time lag from SGR to inhibition of photosynthesis might lead to the accumulation of photosynthates under Md3, which can be used as osmolytes under Sds. To gain further insight into the observed SGR, transcriptome and hormonome analyses were performed in specific tissues. The results showed specific decreases in indole-3-acetic acid (IAA) and cytokinin levels in Md2-, Md3- and Sds-treated shoot bases, though the expression levels of hormone metabolism-related genes were not reflected in IAA and cytokinin contents. These observations suggest that drought stress affects the distribution or degradation of cytokinin and IAA molecules.

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“…Contents of cytokinins were quantified with ultra-performance liquid chromatography-electrospray interface and tandem quadrupole mass spectrometer (AQUITY UPLC System/Xevo-TQS; Waters) as described previously (Kojima et al, 2009). Auxin, JA, and ABA were quantified with ultra-high-performance liquid chromatography (HPLC)-electrospray interface and quadrupole-orbitrap mass spectrometer (UHPLC/Q-Exactive; Thermo Scientific) as described previously Shinozaki et al, 2015).…”

Section: Quantitative Analysis Of Plant Hormonesmentioning

confidence: 99%

Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes

et al. 2017

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Wounding is a primary trigger of organ regeneration, but how wound stress reactivates cell proliferation and promotes cellular reprogramming remains elusive. In this study, we combined transcriptome analysis with quantitative hormonal analysis to investigate how wounding induces callus formation in Arabidopsis (Arabidopsis thaliana). Our time course RNA-seq analysis revealed that wounding induces dynamic transcriptional changes, starting from rapid stress responses followed by the activation of metabolic processes and protein synthesis and subsequent activation of cell cycle regulators. Gene ontology analyses further uncovered that wounding modifies the expression of hormone biosynthesis and response genes, and quantitative analysis of endogenous plant hormones revealed accumulation of cytokinin prior to callus formation. Mutants defective in cytokinin synthesis and signaling display reduced efficiency in callus formation, indicating that de novo synthesis of cytokinin is critical for wound-induced callus formation. We further demonstrate that type-B ARABIDOPSIS RESPONSE REGULATOR-mediated cytokinin signaling regulates the expression of CYCLIN D3;1 (CYCD3;1) and that mutations in CYCD3;1 and its homologs CYCD3;2 and 3 cause defects in callus formation. In addition to these hormone-mediated changes, our transcriptome data uncovered that wounding activates multiple developmental regulators, and we found novel roles of ETHYLENE RESPONSE FACTOR 115 and PLETHORA3 (PLT3), PLT5, and PLT7 in callus generation. All together, these results provide novel mechanistic insights into how wounding reactivates cell proliferation during callus formation.

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Ethylene suppresses tomato (Solanum lycopersicum) fruit set through modification of gibberellin metabolism

Cited by 127 publications

References 62 publications

Suppression of DELLA signaling induces procambial cell formation in culture

Suppression of DELLA signaling induces procambial cell formation in culture

Temporal and spatial changes in gene expression, metabolite accumulation and phytohormone content in rice seedlings grown under drought stress conditions

Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes

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