Ethylene regulates the timing of leaf senescence in <i>Arabidopsis</i>

Grbić, Vojislava; Bleecker, Anthony B.

doi:10.1046/j.1365-313x.1995.8040595.x

Cited by 446 publications

(353 citation statements)

References 25 publications

(40 reference statements)

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“…In contrast, ethylene production accompanying initial hydration of tomato seeds does not significantly alter NR expression and the transient increase in ethylene synthesis observed in tomato seedlings undergoing shoot elongation is not accompanied by changes in NR mRNA accumulation. Similarly, stimulation of NR mRNA is not evident in senescing leaves, where alterations in ethylene sensitivity are well established (Grbic and Bleecker, 1995). Additionally, analysis of RNAs isolated from individual floral explant tissues at anthesis and throughout floral senescence, abscission and fruit set, did not detect any increases in NR mRNA, although significant changes in ethylene metabolism are associated with these processes (Drory et al, 1993).…”

Section: Discussionmentioning

confidence: 98%

Differential regulation of the tomato ETR gene family throughout plant development

1998

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SummaryEthylene perception in plants is co-ordinated by multiple hormone receptor candidates sharing sequence commonalties with prokaryotic environmental sensor proteins known as two-component regulators. Two tomato homologs of the Arabidopsis ethylene receptor ETR1 were cloned from a root cDNA library. Both cDNAs, termed LeETR1 and LeETR2, were highly homologous to ETR1, exhibiting~90% deduced amino acid sequence similarity and 80% deduced amino acid sequence identity. LeETR1 and LeETR2 contained all the major structural elements of two-component regulators, including the response regulator motif absent in LeETR3, the gene encoding tomato NEVER RIPE (NR). Using RNase protection analysis, the mRNAs of LeETR1, LeETR2 and NR were quantified in tissues engaged in key processes of the plant life cycle, including seed germination, shoot elongation, leaf and flower senescence, floral abscission, fruit set and fruit ripening. LeETR1 was expressed constitutively in all plant tissues examined. LeETR2 mRNA was expressed at low levels throughout the plant but was induced in imbibing tomato seeds prior to germination and was down-regulated in elongating seedlings and senescing leaf petioles. NR expression was developmentally regulated in floral ovaries and ripening fruit. Notably, hormonal regulation of NR was highly tissue-specific. Ethylene biosynthesis induced NR mRNA accumulation in ripening fruit but not in elongating seedlings or in senescing leaves or flowers. Furthermore, the abundance of mRNAs for all three LeETR genes remained uniform in multiple plant tissues experiencing marked changes in ethylene sensitivity, including the cell separation layer throughout tomato flower abscission.

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Section: Discussionmentioning

confidence: 98%

Differential regulation of the tomato ETR gene family throughout plant development

1998

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“…Chlorophyll was extracted from leaves and measured according to the protocol of Grbi and Bleecker (1995). The leaves used at each sampling time point were excised from at least three separate plants.…”

Section: Chlorophyll Content and Membrane Ion Leakage Analysismentioning

confidence: 99%

Overexpression of a LAM Domain Containing RNA-Binding Protein LARPIc Induces Precocious Leaf Senescence in Arabidopsis

Zhang

Jia

Yang

et al. 2012

Molecules and Cells

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Leaf senescence is the final stage of leaf life history, and it can be regulated by multiple internal and external cues. La-related proteins (LARPs), which contain a well-conserved La motif (LAM) domain and normally a canonical RNA recognition motif (RRM) or noncanonical RRM-like motif, are widely present in eukaryotes. Six LARP genes (LARP1a-1c and LARP6a-6c) are present in Arabidopsis, but their biological functions have not been studied previously. In this study, we investigated the biological roles of LARP1c from the LARP1 family. Constitutive or inducible overexpression of LARP1c caused premature leaf senescence. Expression levels of several senescence-associated genes and defense-related genes were elevated upon overexpression of LARP1c. The LARP1c null mutant 1c-1 impaired ABA-, SA-, and MeJA-induced leaf senescence in detached leaves. Gene expression profiles of LARP1c showed age-dependent expression in rosette leaves. Taken together, our results suggest LARP1c is involved in regulation of leaf senescence.

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“…Cytokinins have been found to play a role in delaying leaf senescence (Gan and Amasino, 1995;McCabe et al, 2001;Kim et al, 2006), whereas ethylene, jasmonates (JAs), brassinosteroids, and salicylic acids are known to promote the process of senescence (Grbic and Bleecker, 1995;Oh et al, 1997;Morris et al, 2000;Jing et al, 2002;Yin et al, 2002). For example, JA could rapidly induce the expression of several SAGs (Park et al, 1998;Schenk et al, 2000).…”

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confidence: 99%

A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice

et al. 2006

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Leaf senescence is a developmentally programmed degeneration process, which is fine tuned by a complex regulatory network for plant fitness. However, molecular regulation of leaf senescence is poorly understood, especially in rice (Oryza sativa), an important staple crop for more than half of the world population. Here, we report a novel nuclear-localized CCCH-type zinc finger protein, Oryza sativa delay of the onset of senescence (OsDOS), involved in delaying leaf senescence in rice. The expression of OsDOS was down-regulated during natural leaf senescence, panicle development, and pollination, although its transcripts were accumulated in various organs. RNAi knockdown of OsDOS caused an accelerated age-dependent leaf senescence, whereas its overexpression produced a marked delay of leaf senescence, suggesting that it acts as a negative regulator for leaf senescence. A genome-wide expression analysis further confirmed its negative regulation for leaf senescence and revealed that, in particular, the jasmonate (JA) pathway was found to be hyperactive in the OsDOS RNAi transgenic lines but impaired in the OsDOS overexpressing transgenic lines, indicating that this pathway is likely involved in the OsDOS-mediated delaying of leaf senescence. Furthermore, methyl JA treatments of both seeds and detached leaves from the RNAi and the overexpressing transgenic lines showed hyperand hyporesponses, respectively, consistent with the negative regulation of the JA pathway by OsDOS. Together, these results indicate that OsDOS is a novel nuclear protein that delays leaf senescence likely, at least in part, by integrating developmental cues to the JA pathway.

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Ethylene regulates the timing of leaf senescence in Arabidopsis

Cited by 446 publications

References 25 publications

Differential regulation of the tomato ETR gene family throughout plant development

Differential regulation of the tomato ETR gene family throughout plant development

Overexpression of a LAM Domain Containing RNA-Binding Protein LARPIc Induces Precocious Leaf Senescence in Arabidopsis

A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice

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