Ethylene receptors and genetic engineering of ethylene sensitivity in plants.

Sato‐Nara, Kumi; Yuhashi, Ken Ichi; Ezura, Hiroshi

doi:10.5511/plantbiotechnology.16.321

Cited by 6 publications

(1 citation statement)

References 51 publications

(28 reference statements)

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“…Homologues of ethylene receptor genes have been isolated from tomato, tobacco and some other plants (Sato-Nara et al 1999a). In tomato, five ethylene receptor genes namely LeETR1, LeETR2, NR, LeETR4 and LeETR5 have been identified and their gene expressions in defense responses investigated (Wilkinson et al 1995, Zhou et al 1996, Lashbrook et al 1998, Tieman and Klee 1999, Ciardi et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Spatial Expression and Characterization of a Putative Ethylene Receptor Protein NTHK1 in Tobacco

Xie

Zhang

et al. 2002

Plant and Cell Physiology

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A putative ethylene receptor gene NTHK1 encodes a protein with a putative signal peptide, three transmembrane segments, a putative histidine kinase domain and a putative receiver domain. The receiver domain was expressed in an Escherichia coli expression system, purified and used to generate polyclonal antibodies for immunohistochemistry analysis. The spatial expression of the NTHK1 protein was then investigated. We found that NTHK1 was abundant during flower and ovule development. It was also expressed in glandular hairs, stem, and in leaves that had been wounded. The NTHK1 gene was further introduced into the tobacco plant and we found that, in different transgenic lines, the NTHK1 gene was transcribed to various degrees. Upon ACC treatment, the etiolated transgenic seedlings showed reduced ethylene sensitivity when compared with the control, indicating that NTHK1 is a functional ethylene receptor in plants.

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