Ethylene is Involved in the Control of Male Gametophyte Development and Germination in Petunia

Ковалева, Л. В.; Dobrovol'skaya, A. A.; Воронков, А. С.; Rakitin, V. Yu.

doi:10.1007/s00344-010-9168-6

Cited by 17 publications

(13 citation statements)

References 34 publications

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“…Furthermore, plant hormones are important signaling molecules that regulate anther development. A series of plant hormones, such as ethylene and gibberellin (GA), play key roles in the regulation of anther development in plants (Thornsberry et al 2001;Kovaleva et al 2011). In our transcriptome study, GO and KEGG enrichment analysis revealed that the DEGs were significantly enriched in plant hormone signal transduction pathways.…”

Section: The Abnormal Metabolisms Of Starch and Sucrose May Be An Impmentioning

confidence: 82%

Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing

Wei

et al. 2020

Mol Genet Genomics

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Genetic male sterility (GMS) facilitates hybrid seed production in crops including cotton (Gossypium hirsutum). However, the genetic and molecular mechanisms specifically involved in this developmental process are poorly understood. In this study, small RNA sequencing, degradome sequencing, and transcriptome sequencing were performed to analyze miRNAs and their target genes during anther development in a GMS mutant ('Dong A') and its fertile wildtype (WT). A total of 80 known and 220 novel miRNAs were identified, 71 of which showed differential expressions during anther development. A further degradome sequencing revealed a total of 117 candidate target genes cleaved by 16 known and 36 novel miRNAs. Based on RNA-seq, 24, 11, and 21 predicted target genes showed expression correlations with the corresponding miRNAs at the meiosis, tetrad and uninucleate stages, respectively. In addition, a large number of differentially expressed genes were identified, most of which were involved in sucrose and starch metabolism, carbohydrate metabolism, and plant hormone signal transduction based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The results of our study provide valuable information for further functional investigations of the important miRNAs and target genes involved in genetic male sterility and advance our understanding of miRNA regulatory functions during cotton anther development.

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Section: The Abnormal Metabolisms Of Starch and Sucrose May Be An Impmentioning

confidence: 82%

Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing

Wei

et al. 2020

Mol Genet Genomics

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“…In anther development, ethylene is important for male gametophyte germination and anther dehiscence [43,44] and it has been reported that fertile male gametophyte development is accompanied by two peaks of ethylene production in anther tissues and the mature pollen is characterized by a high content of ethylene [45]. In the current study, Gh-miR396 was differentially expressed between ‘Dong A’ WT and its GMS mutant anthers in the meiosis stage, and it had a higher level of expression in the GMS mutant anthers in the uninucleate microspore stage.…”

Section: Discussionmentioning

confidence: 99%

Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton

Wei

et al. 2013

BMC Plant Biol

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BackgroundGenetic male sterility (GMS) in cotton (Gossypium hirsutum) plays an important role in the utilization of hybrid vigor. However, the molecular mechanism of the GMS is still unclear. While numerous studies have demonstrated that microRNAs (miRNA) regulate flower and anther development, whether different small RNA regulations exist in GMS and its wild type is unclear. A deep sequencing approach was used to investigate the global expression and complexity of small RNAs during cotton anther development in this study.ResultsThree small RNA libraries were constructed from the anthers of three development stages each from fertile wild type (WT) and its GMS mutant cotton, resulting in nearly 80 million sequence reads. The total number of miRNAs and short interfering RNAs in the three WT libraries was significantly greater than that in the corresponding three mutant libraries. Sixteen conserved miRNA families were identified, four of which comprised the vast majority of the expressed miRNAs during anther development. In addition, six conserved miRNA families were significantly differentially expressed during anther development between the GMS mutant and its WT.ConclusionsThe present study is the first to deep sequence the small RNA population in G. hirsutum GMS mutant and its WT anthers. Our results reveal that the small RNA regulations in cotton GMS mutant anther development are distinct from those of the WT. Further results indicated that the differently expressed miRNAs regulated transcripts that were distinctly involved in anther development. Identification of a different set of miRNAs between the cotton GMS mutant and its WT will facilitate our understanding of the molecular mechanisms for male sterility.

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“…The mature pollen is characterized by a high content of ethylene. During the anther development, fertile male gametophyte development is accompanied by two peaks of ethylene production by anther tissues [41]. The first peak occurs during microspore development simultaneously with degeneration of both tapetal tissues and the middle layers of the anther wall.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of differentially expressed genes during anther development in genetic male sterile and wild type cotton by digital gene-expression profiling

et al. 2013

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BackgroundCotton (Gossypium hirsutum) anther development involves a diverse range of gene interactions between sporophytic and gametophytic tissues. However, only a small number of genes are known to be specifically involved in this developmental process and the molecular mechanism of the genetic male sterility (GMS) is still poorly understand. To fully explore the global gene expression during cotton anther development and identify genes related to male sterility, a digital gene expression (DGE) analysis was adopted.ResultsSix DGE libraries were constructed from the cotton anthers of the wild type (WT) and GMS mutant (in the WT background) in three stages of anther development, resulting in 21,503 to 37,352 genes detected in WT and GMS mutant anthers. Compared with the fertile isogenic WT, 9,595 (30% of the expressed genes), 10,407 (25%), and 3,139 (10%) genes were differentially expressed at the meiosis, tetrad, and uninucleate microspore stages of GMS mutant anthers, respectively. Using both DGE experiments and real-time quantitative RT-PCR, the expression of many key genes required for anther development were suppressed in the meiosis stage and the uninucleate microspore stage in anthers of the mutant, but these genes were activated in the tetrad stage of anthers in the mutant. These genes were associated predominantly with hormone synthesis, sucrose and starch metabolism, the pentose phosphate pathway, glycolysis, flavonoid metabolism, and histone protein synthesis. In addition, several genes that participate in DNA methylation, cell wall loosening, programmed cell death, and reactive oxygen species generation/scavenging were activated during the three anther developmental stages in the mutant.ConclusionsCompared to the same anther developmental stage of the WT, many key genes involved in various aspects of anther development show a reverse gene expression pattern in the GMS mutant, which indicates that diverse gene regulation pathways are involved in the GMS mutant anther development. These findings provide the first insights into the mechanism that leads to genetic male sterility in cotton and contributes to a better understanding of the regulatory network involved in anther development in cotton.

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Ethylene is Involved in the Control of Male Gametophyte Development and Germination in Petunia

Cited by 17 publications

References 34 publications

Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing

Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing

Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton

Transcriptomic analysis of differentially expressed genes during anther development in genetic male sterile and wild type cotton by digital gene-expression profiling

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