Ethylene-induced gene expression, enzyme activities, and water soaking in immature and ripe watermelon (Citrullus lanatus) fruit

Karakurt, Yaşar; Huber, Donald J.

doi:10.1078/0176-1617-01221

Cited by 32 publications

(24 citation statements)

References 31 publications

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“…Ethylene plays a major role in regulating the expression of cell wall-associated genes encoding PGs (Sitrit and Bennett, 1998; Hiwasa et al, 2004), EXPs (Rose et al, 1997; Hiwasa et al, 2003) and BGals (Karakurt and Huber, 2004; Nishiyama et al, 2007). Therefore, we suggest that an extensive up-regulation of cell wall-related DEGs in the abscising pods could, to some extent, be the result of an increased ethylene biosynthesis in these organs.…”

Section: Discussionmentioning

confidence: 99%

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

et al. 2017

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Yellow lupine (Lupinus luteus L., Taper c.), a member of the legume family (Fabaceae L.), has an enormous practical importance. Its excessive flower and pod abscission represents an economic drawback, as proper flower and seed formation and development is crucial for the plant's productivity. Generative organ detachment takes place at the basis of the pedicels, within a specialized group of cells collectively known as the abscission zone (AZ). During plant growth these cells become competent to respond to specific signals that trigger separation and lead to the abolition of cell wall adhesion. Little is known about the molecular network controlling the yellow lupine organ abscission. The aim of our study was to establish the divergences and similarities in transcriptional networks in the pods, flowers and flower pedicels abscised or maintained on the plant, and to identify genes playing key roles in generative organ abscission in yellow lupine. Based on de novo transcriptome assembly, we identified 166,473 unigenes representing 219,514 assembled unique transcripts from flowers, flower pedicels and pods undergoing abscission and from control organs. Comparison of the cDNA libraries from dropped and control organs helped in identifying 1,343, 2,933 and 1,491 differentially expressed genes (DEGs) in the flowers, flower pedicels and pods, respectively. In DEG analyses, we focused on genes involved in phytohormonal regulation, cell wall functioning and metabolic pathways. Our results indicate that auxin, ethylene and gibberellins are some of the main factors engaged in generative organ abscission. Identified 28 DEGs common for all library comparisons are involved in cell wall functioning, protein metabolism, water homeostasis and stress response. Interestingly, among the common DEGs we also found an miR169 precursor, which is the first evidence of micro RNA engaged in abscission. A KEGG pathway enrichment analysis revealed that the identified DEGs were predominantly involved in carbohydrate and amino acid metabolism, but some other pathways were also targeted. This study represents the first comprehensive transcriptome-based characterization of organ abscission in L. luteus and provides a valuable data source not only for understanding the abscission signaling pathway in yellow lupine, but also for further research aimed at improving crop yields.

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Section: Discussionmentioning

confidence: 99%

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

et al. 2017

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“…LOX-mediated fatty acid oxidation is initiated during the early stages of plant senescence, resulting in membrane breakdown and loss of cellular compartmentalization (Thompson et al, 1998). Increased LOX activity has been suggested to be associated with loss of firmness during fruit ripening (Wu et al, 1999), and stimulation of LOX gene expression has been found in ripening fruit such as tomato (Griffiths et al, 1999), apple (Schaffer et al, 2007), and watermelon (Karakurt and Huber, 2004). In this study, postharvest UV-C irradiation significantly suppressed the expression of LOX gene, thus might delayed fruit softening and ripening.…”

Section: Genes Involved In Cell Wall Disassemble and Lipid Metabolismmentioning

confidence: 99%

Temporary effect of postharvest UV-C irradiation on gene expression profile in tomato fruit

Liu

Cai

Han

et al. 2011

Gene

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“…Watermelon Promotion Board 2003). Like other fruit, fresh‐cut watermelon deteriorates faster than the whole fruit due to the wounding that occurs during processing (Watada and others 1990, 1996) as well as physiological changes that occur postprocessing (Karakurt and Huber 2004; Mao and others 2004, 2006). Although watermelon is a nonclimacteric fruit (Karakurt and Huber 2002) and usually produces little ethylene (Elkashif and others 1989), its physiology and quality are affected by low concentrations of exogenous ethylene to which the watermelon may be exposed during transportation and storage.…”

Section: Introductionmentioning

confidence: 99%

1‐Methylcyclopropene Counteracts Ethylene‐Induced Microbial Growth on Fresh‐Cut Watermelon

Zhou

McEvoy

Luo

et al. 2006

Journal of Food Science

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The effects of exogenous ethylene, 1‐methylcyclopropene (1‐MCP), or both on microbial growth on watermelon fruit and watermelon slices were investigated. Freshly harvested seedless watermelons (Citrullus lanatus, cv. Sugar Heart) were treated with 0.5 or 1.0 ppm 1‐MCP, 10 ppm ethylene, 1‐MCP + ethylene, or left untreated as controls. Fruits were processed into wedge‐shaped slices, packaged into rigid trays sealed with a polyethylene film with a 29.2 pmol s−1 m−2 Pa−1 oxygen transmission rate. The slices were evaluated after 0‐, 6‐, and 12‐d storage at 5 °C. Ethylene treatment alone increased the populations of aerobic bacteria, lactic acid bacteria, and yeasts and molds on the packaged slices during storage compared to those on corresponding control slices and resulted in extensive juice leakage from the slices. The ethylene treatment also resulted in high aerobic bacterial counts throughout the flesh of whole melons compared to the controls. Treating watermelons with 0.5 or 1.0 ppm 1‐MCP prior to ethylene exposure counteracted the deleterious effects of ethylene. Extending the time from harvest to 1‐MCP treatment increased the population of aerobic bacteria, but had no detectable effect on the growth of lactic acid bacteria or yeasts and molds. The results indicate that low concentrations (0.5 or 1.0 ppm) of 1‐MCP can be used on whole watermelon to avoid deleterious effects of exogenous ethylene to which the melons could be exposed during shipping or storage.

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Ethylene-induced gene expression, enzyme activities, and water soaking in immature and ripe watermelon (Citrullus lanatus) fruit

Cited by 32 publications

References 31 publications

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

Temporary effect of postharvest UV-C irradiation on gene expression profile in tomato fruit

1‐Methylcyclopropene Counteracts Ethylene‐Induced Microbial Growth on Fresh‐Cut Watermelon

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