Ethylene Does Not Promote Adventitious Root Initiation on Apple Microcuttings

Harbage, James F.; Stimart, Dennis P.

doi:10.21273/jashs.121.5.880

Cited by 15 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is difficult to directly compare the results of Harbage and Stimart (1996) with our own. Although there was no effect of ethylene on apple rooting, they reported only root number with no information on rooting percentage, root elongation or callus formation.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we tested the effects of ACC, AgNO 3 , AVG and CoCl 2 on root regeneration from shoot cultures of the apple cultivar Royal Gala, for which we have developed a transformation system (Yao et al 1995). After these experiments were completed, Harbage and Stimart (1996) published results suggesting that ethylene was not associated with the rooting of apple microcuttings.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ethylene inhibitors enhance in vitro root formation from apple shoot cultures

Ma¹,

Yao²,

Cohen³

et al. 1998

Plant Cell Reports

View full text Add to dashboard Cite

Effects of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and three ethylene inhibitors, AgNO 3 , aminoethoxyvinyglycine (AVG) and CoCl 2 , on root formation were tested in vitro using shoot cultures of the apple (Malus×domestica Borkh.) cultivar Royal Gala. ACC inhibited root formation by delaying root emergence and increasing callus formation at the bases of shoots. In contrast, ethylene inhibitors promoted root formation. Both AgNO 3 and AVG at the appropriate concentrations increased the percentage of shoots producing roots and reduced callus formation at the base of these shoots. AgNO 3 stimulated root emergence and enhanced root growth, while AVG increased the number of roots per shoot. CoCl 2 slightly increased root number and rooting efficiency. These promotive effects may result from a reduction in ethylene concentration or inhibition of ethylene action. The results found in this study may be used to improve the rooting efficiency of other apple cultivars and rootstocks, and possibly of other plant species.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ethylene inhibitors enhance in vitro root formation from apple shoot cultures

Ma¹,

Yao²,

Cohen³

et al. 1998

Plant Cell Reports

View full text Add to dashboard Cite

show abstract

“…Ethylene response has been shown very specifically, and conflicting responses have been found from the same species (Harbage and Stimart 1996;Ma et al 1998). Hence it follows that the effects of ethylene vary markedly between species or even within the same species.…”

Section: Resultsmentioning

confidence: 99%

Involvement of ethylene in the rooting of seedling shoot cultures of Bixa orellana L.

Neto

Reis

Finger

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Using seedlings derived from the shoot apex of annatto (Bixa orellana L. cv. Bico-de-Pato) we observed the rooting frequency of B. orellana, the number and length of roots and the rate of ethylene production during 30 d in culture. The rhizogenesis response was affected by auxins (NAA or IBA) and by both the ethylene biosynthesis precursor 1-aminocyclopropane-1-carboxylic acid (ACC) and the inhibitor 2-aminoethoxyvinylglycine (AVG). Auxin supplementation to the medium resulted in root induction, ethylene production, and an undesirable callusing in the epidermal and cortical tissues. Irrespective of the presence of auxins, supplementing the medium with ACC promoted ethylene biosynthesis and callusing, which resulted in increased cell proliferation mainly in the cortical and vascular tissues, while the epidermis was mostly unaltered. In both ACC and auxin-supplemented medium, increased ethylene production and callusing occurred, suggesting a synergistic effect between these two responses. ACC was capable of inducing adventitious root formation, but the roots produced had a wrinkled appearance when compared to normal roots. Conversely, AVG reduced ethylene production and callusing, while the epidermis, cortex, and inner tissues remained unaltered, regardless of the presence of auxins. AVG was beneficial in these aspects, although its application led to a reduction in the number of roots and in the average root length. In conclusion, it was not possible to establish a direct relation between ethylene and rooting, but we hypothesize that, under the experimental conditions described, ethylene may enhance tissue sensitivity to auxin. However, ethylene did not seem essential to the rhizogenesis process in annatto.

show abstract

“…We hypothesized that ethephon exerts its effect on increasing shoot formation in 'Little Pinkie' through its decomposition to ethylene in the headspace of culture vessels, and thus investigated the putative effects of ethephon and ethylene on the plants in a culture vessel capable of gas exchange. Previous studies on the presence of ethylene in vitro have merely described the negative/positive effects of ethylene/ethephon on growth variables of plants (Harbage and Stimart, 1996;Kerbauy and Colli, 1997), in contrast to detailing an ethephon-ethylene relationship within an unsealed culture vessel used for in vitro culture. The culture vessels used in our study needed to be unsealed to allow for gas exchange during plant growth in vitro.…”

mentioning

confidence: 99%

Is Endogenous Ethylene Production Associated with Increased Shoot Number in Gentian Cultured In Vitro? Developing a Method for Ethylene Measurement at Nano Level within a Gas-exchangeable In Vitro System

Keshavarzi

Funnell

Woolley

et al. 2021

horts

View full text Add to dashboard Cite

We investigated the possibility of either exogenous ethylene or endogenous ethylene production having an association with the increase in shoot number when nodal explants of Gentiana spp. ‘Little Pinkie’ were cultured in an in vitro medium supplemented with ethephon (10 mg⋅L–1). For the first time within an in vitro system, we report the application of laser ethylene detector technology, and optimization of the methodology to quantify concentrations of ethylene (in the part-per-billion range) released from ethephon decomposition within the atmosphere of gas-exchangeable culture vessels including nodal explants. Compared with continuous (continuous measurements on the same replicate of vessels) and repeated (sampling same replicate of vessels every 48 hours) sampling methodologies, the nonrepeated (sampling fresh replicate of vessels every 48 hours) method of measurement of ethylene concentration was more representative of the actual condition within vessels. Although no prior published data exist showing the positive or negative effect of gaseous ethylene in the headspace of culture vessels on bud outgrowth in gentian, our study shows gaseous ethylene in the headspace of culture vessels was not effective in increasing shoot formation in gentian explants cultured in vitro, whereas ethephon supplementation in agar was effective. Plant material in culture vessels did not have a significant effect on ethylene production regardless of the presence or absence of ethephon. Therefore, although ethephon supplementation in the medium produced gaseous ethylene in the headspace, it was unlikely to cause endogenous ethylene production in explants, but it did trigger shoot formation in ‘Little Pinkie’, perhaps through decomposition to ethylene within the explant tissue, enhancing the internal ethylene level possibly at a locally high concentration.

show abstract

Ethylene Does Not Promote Adventitious Root Initiation on Apple Microcuttings

Cited by 15 publications

References 19 publications

Ethylene inhibitors enhance in vitro root formation from apple shoot cultures

Ethylene inhibitors enhance in vitro root formation from apple shoot cultures

Involvement of ethylene in the rooting of seedling shoot cultures of Bixa orellana L.

Is Endogenous Ethylene Production Associated with Increased Shoot Number in Gentian Cultured In Vitro? Developing a Method for Ethylene Measurement at Nano Level within a Gas-exchangeable In Vitro System

Contact Info

Product

Resources

About