Ethylene directs auxin to control root cell expansion

Strader, Lucia C.; Chen, Grace L.; Bartel, Bonnie

doi:10.1111/j.1365-313x.2010.04373.x

Cited by 152 publications

(100 citation statements)

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“…The mechanisms of synergistic inhibition of root elongation by ethylene and auxin have been described previously (Ruzicka et al, 2007;Stepanova et al, 2007;Strader et al, 2010;Swarup et al, 2007). This work explores the mechanisms of auxin and ethylene crosstalk, in a very different scenario in which auxin and ethylene act antagonistially, rather than synergistically.…”

Section: Discussionmentioning

confidence: 99%

“…Mutations in CTR1, which confer constitutive ethylene signaling (Huang et al, 2003;Kieber et al, 1993), and the ethylene overproducing eto1 mutant (Guzman and Ecker, 1990), both negatively affect root branching (Negi et al, 2008;Strader et al, 2010). Additionally, treatment with ethylene or the ethylene precursor, 1-aminocyclopropane carboxylic acid (ACC) reduces lateral root formation in both species (Negi et al, 2008;Negi et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Ethylene inhibits lateral root development, increases IAA transport and expression of PIN3 and PIN7 auxin efflux carriers

et al. 2011

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SUMMARYWe used genetic and molecular approaches to identify mechanisms by which the gaseous plant hormone ethylene reduces lateral root formation and enhances polar transport of the hormone auxin. Arabidopsis thaliana mutants, aux1, lax3, pin3 and pin7, which are defective in auxin influx and efflux proteins, were less sensitive to the inhibition of lateral root formation and stimulation of auxin transport following treatment with the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC). By contrast, pin2 and abcb19 mutants exhibited wild-type ACC responses. ACC and indole-3-acetic acid (IAA) increased the abundance of transcripts encoding auxin transport proteins in an ETR1 and EIN2 (ethylene signaling)-dependent and TIR1 (auxin receptor)-dependent fashion, respectively. The effects of ACC on these transcripts and on lateral root development were still present in the tir1 mutant, suggesting independent signaling networks. ACC increased auxin-induced gene expression in the root apex, but decreased expression in regions where lateral roots form and reduced free IAA in whole roots. The ethylene synthesis inhibitor aminoethoxyvinylglycine (AVG) had opposite effects on auxin-dependent gene expression. These results suggest that ACC affects root development by altering auxin distribution. PIN3-and PIN7-GFP fluorescence was increased or decreased after ACC or AVG treatment, respectively, consistent with the role of PIN3 and PIN7 in ACC-elevated transport. ACC treatment abolished a localized depletion of fluorescence of PIN3-and PIN7-GFP, normally found below the site of primordia formation. These results suggest that ACC treatment increased PIN3 and PIN7 expression, resulting in elevated auxin transport, which prevented the localized accumulation of auxin needed to drive lateral root formation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ethylene inhibits lateral root development, increases IAA transport and expression of PIN3 and PIN7 auxin efflux carriers

et al. 2011

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“…Auxin is responsible for controlling the process of cell elongation and growth and differentiation of primary and vascular tissues, such as metaxylem and protoxylem (Aloni et al, 2006). Furthermore, this hormone is responsible for leading various processes of root growth, such as tropic responses to light and gravity, the general architecture of plants and cell growth (Strader et al, 2010;Tanimoto, 2005).…”

Section: Discussionmentioning

confidence: 99%

Phytotoxic potential of young leaves from Blepharocalyx salicifolius (Kunth) O. Berg (Myrtaceae)

et al. 2016

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The loss of leaves by plant species found in the Cerrado (Brazilian savanna) is an energetically expensive process due to adverse environmental conditions and predation by herbivory. The mature leaves have adaptations which minimize these events. However, the young individuals lack these structures and produce high leaf concentrations of secondary metabolites as a form of protection. These compounds can be used in bioprospection of natural herbicides. Thus, this study aimed to evaluate the phytotoxicity of hexane, ethyl acetate and aqueous extracts of young leaves from Blepharocalyx salicifolius (Kunth) O. Berg on the elongation of wheat coleoptiles (Triticum aestivum L.) and evaluate the potential phytotoxic of ethyl acetate extract on germination, growth and cell size of metaxylem of sesame (Sesamum indicum L.) seedlings. The hexane and ethyl acetate extracts inhibited the elongation of wheat coleoptiles at all concentrations; however, the most promising results were observed in coleoptile fragments treated with the ethyl acetate extract. This treatment changed the mean germination time and the synchrony of sesame seeds, inhibited the growth of shoots and roots, reduced the dry weight of seedlings, led to abnormalities in the seedlings and reduced the length of the metaxylem cells in the sesame seedlings. These results demonstrated the phytotoxic potential of young leaf extracts of B. salicifolius and the high phytotoxicity of the ethyl acetate extract in the initial development of S. indicum.Keywords: Triticum aestivum, Sesamum indicum, murta, allelopathy, bioactivity. Potencial fitotóxico de folhas jovens de

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“…(A) PCR-based genotyping of abp1-5, Col-0, Ws-2, and Ler-0 using mapping markers nga162 (Bell and Ecker, 1994), nga112 (Bell and Ecker, 1994), GLL310 (Strader et al, 2010), GLL320, and LCS332. See Table 2 for genotyping information.…”

Section: Accession Numbersmentioning

confidence: 99%

Genome Sequencing of Arabidopsis abp1-5 Reveals Second-Site Mutations That May Affect Phenotypes

Enders

Yang

et al. 2015

The Plant Cell

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Auxin regulates numerous aspects of plant growth and development. For many years, investigating roles for AUXIN BINDING PROTEIN1 (ABP1) in auxin response was impeded by the reported embryo lethality of mutants defective in ABP1. However, identification of a viable Arabidopsis thaliana TILLING mutant defective in the ABP1 auxin binding pocket (abp1-5) allowed inroads into understanding ABP1 function. During our own studies with abp1-5, we observed growth phenotypes segregating independently of the ABP1 lesion, leading us to sequence the genome of the abp1-5 line described previously. We found that the abp1-5 line we sequenced contains over 8000 single nucleotide polymorphisms in addition to the ABP1 mutation and that at least some of these mutations may originate from the Arabidopsis Wassilewskija accession. Furthermore, a phyB null allele in the abp1-5 background is likely causative for the long hypocotyl phenotype previously attributed to disrupted ABP1 function. Our findings complicate the interpretation of abp1-5 phenotypes for which no complementation test was conducted. Our findings on abp1-5 also provide a cautionary tale illustrating the need to use multiple alleles or complementation lines when attributing roles to a gene product. INTRODUCTIONThe plant hormone auxin regulates cell division and cell expansion to affect all aspects of plant growth (reviewed in PerrotRechenmann, 2010). Auxin regulates a wide range of developmental processes, and tight control of auxin response is maintained by multiple modes of regulation. These include regulating auxin biosynthesis and metabolism, transport, and signaling (reviewed in Enders and Strader, 2015). To date, nuclear auxin signaling components have been well characterized (reviewed in Chapman and Estelle, 2009;Salehin et al., 2015). In addition, a nontranscriptional auxin response pathway has been proposed, with AUXIN BINDING PROTEIN1 (ABP1) acting in the apoplast as an auxin receptor and transmitting a cytoplasmic signal to regulate auxin responses such as auxin transport and cytoskeletal rearrangements (reviewed in Shi and Yang, 2011;Sauer et al., 2013).Study of ABP1 has a long, complicated history. Experiments demonstrating auxin binding activity for ABP1 were published as early as the 1980s (reviewed in Jones, 1994). Reverse genetics proved to be a complicated approach to study ABP1 function in Arabidopsis thaliana, as two independently generated abp1 mutants, presumed to be null alleles, appeared to be embryo lethal (Chen et al., 2001;Tzafrir et al., 2004;Meinke et al., 2008;Sassi et al., 2014). Knockdown lines of ABP1 provided some ability to study ABP1 function by reverse genetics. Arabidopsis lines expressing an inducible ABP1 antisense transcript or an inducible single-chain fragment variable scFv12 (David et al., 2007) from a monoclonal antibody raised to ABP1 (Leblanc et al., 1999), targeted to either the apoplast (SS12S) or the endoplasmic reticulum (SS12K), led to phenotypes consistent with decreased auxin activity (Braun et al., 2008;Tromas et al.,...

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Ethylene directs auxin to control root cell expansion

Cited by 152 publications

References 68 publications

Ethylene inhibits lateral root development, increases IAA transport and expression of PIN3 and PIN7 auxin efflux carriers

Ethylene inhibits lateral root development, increases IAA transport and expression of PIN3 and PIN7 auxin efflux carriers

Phytotoxic potential of young leaves from Blepharocalyx salicifolius (Kunth) O. Berg (Myrtaceae)

Genome Sequencing of Arabidopsis abp1-5 Reveals Second-Site Mutations That May Affect Phenotypes

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