Ethylene-dependent regulation of an α-l-arabinofuranosidase is associated to firmness loss in ‘Gala’ apples under long term cold storage

Storch, Tatiane Timm; Finatto, Taciane; Pegoraro, Camila; Cero, Joceani Dal; Laurens, François; Rombaldi, César Valmor; Quecini, Vera; Girardi, C. L.

doi:10.1016/j.foodchem.2015.02.123

Cited by 15 publications

(30 citation statements)

References 35 publications

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“…In control fruit, the expression of MdACO1 was as described previously, with increased expression along maturation ( Figure 2) (Binnie & McManus, 2009;Ireland et al, 2012;Wiersma et al, 2007;Yang et al, 2013;Zhu et al, 2008), (Figure 2), in agreement with the effective role of the inhibitor in preventing ethylene biosynthesis in apples (Watkins, 2006(Watkins, , 2008 (Storch et al, 2015b). Although the use of 1-MCP is known to reduce ethylene synthesis, basal levels of the hormone may occur (Hiwasa et al, 2003).…”

Section: Experiments IIsupporting

confidence: 84%

“…The observed behavior of MdATT2 suggests that the (Figure 2), confirming the positive regulation of their transcription by ethylene (Tatsuki et al, 2011;Wakasa et al, 2006). Similar results were obtained for MdAF3 and MdAF1 (Storch et al, 2015b) � .…”

Section: Experiments IIsupporting

confidence: 81%

“…was inversely correlated to pulp firmness, so that higher accumulation of MdPG1, MdAF1 and MdAF3 transcripts coincided with lower firmness (Storch et al, 2015b). This behavior suggests that the enzymes coded by MdPG1, MdAF1 and Relative mRNA abundance in 'Gala' apple (Malus x domestica) fruit in different storage conditions.…”

Section: The Expression Of Mdpg1 Mdaf3 and Mdaf1mentioning

confidence: 92%

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Identification of biomarkers associated to ‘Gala’ apples ripening and postharvest quality

et al. 2017

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Apple is, sociocultural and economically, on of the most important species in the world, and stands out for its high storage potential. However, the monitoring of factors that could result in fruit quality modifications during postharvest is essential to ensure the acceptability and for the development of new storage technologies in order to increase fruit shelf life. Approaches focused on molecular biology, such as RT-qPCR have been used to better understand the mechanisms involved in fruit development and maturation. In this study the use of RT-qPCR to monitoring apple quality during ripening and development in different postharvest conditions such as room temperature, cold storage with or without control of atmosphere and the 1-methylcyclopropene usage were proposed. The potential of genes involved in ethylene biosynthesis and response, cell wall modification and degradation, sugar and aroma metabolisms for employment as biomarkers of fruit development and quality were evaluated. Thus MdEXP4 is highlighted as biomarker for development and MdACO1, MdPG1, MdAF1, MdAF3 and MdAAT2 as potential biomarkers for ripening. MdACO1 and MdPG1 appear as suitable markers for quality, conservation technologies and storage time in apples. This work suggests that the study of gene expression by RT-qPCR may be an alternative for a better fruit characterization during the development and postharvest period.

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Section: Experiments IIsupporting

confidence: 84%

Section: Experiments IIsupporting

confidence: 81%

Section: The Expression Of Mdpg1 Mdaf3 and Mdaf1mentioning

confidence: 92%

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Identification of biomarkers associated to ‘Gala’ apples ripening and postharvest quality

et al. 2017

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“…Ethylene synthesis in the 1-MCP treatments was between 5.3-and 15-fold lower than the untreated fruits after 70 days of storage and between 2.1-and 66-fold lower than the untreated fruits after 140 days of storage at 0 ∘ C followed by 7 days at 20 ∘ C (Table 2). Studies have shown that ethylene synthesis changes during long-term storage of apples [23,32,33]. However, results differed, depending on cultivar and storage conditions.…”

Section: Resultsmentioning

confidence: 99%

Influence of 1-Methylcyclopropene Treatment on Postharvest Quality of Four Scab (Venturia inaequalis)-Resistant Apple Cultivars

Zucoloto

Kim

et al. 2017

Journal of Food Quality

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Scab (Venturia inaequalis) is a very serious disease for apples causing up to 80% of loss in yield but there are only a few studies on postharvest quality of scab-resistant cultivars. In this study we evaluated the effect of 1-methylcyclopropene (1-MCP) on fruit quality, total phenolic content, and antioxidant capacity after storage of four scab-resistant cultivars and compared to a standard cultivar, "Golden Delicious." In general, ethylene production and respiration rates significantly differed among cultivars, between control and 1-MCP-treated fruits, and between storage duration regimes. 1-MCP treatment retarded fruit softening and lowered juice pH but storage effect on soluble solids and acidity depended on cultivar and 1-MCP treatment. Total phenolic content was significantly affected by storage duration and 1-MCP treatment. Antioxidant capacity of the four scab-resistant cultivars was either similar to or significantly higher than that of "Golden Delicious" with the 1-MCP-treated fruits having significantly higher antioxidant capacity than the nontreated fruits after storage. Our results clearly show that the quality of four scab-resistant cultivars was comparable to that of "Golden Delicious" and 1-MCP effect differed among cultivars. These differences need to be considered in developing storage regime to minimize quality deterioration during long-term storage. IntroductionThe hazardous effects of pesticides on human health and the environment have contributed to the introduction of cultivars with effective resistance genes to major plant diseases. Yield losses from fungal diseases are significant in most apple producing countries worldwide. Apple scab, caused by the fungus Venturia inaequalis, is one of the most destructive diseases of apples throughout the world, causing up to 80% loss in yield without chemical intervention, which could include multiple classes of fungicides applied up to 22 times during the growing season [1]. In 1944 Hough, from University of Illinois, was the first to identify the scab-resistant gene in Malus floribunda [2]. Since 1944, additional scab-resistant genes have been identified in other apple species [3]. In 1948, three US universities (Purdue, Rutgers, and Illinois, PRI) formed a research team to develop apple cultivars resistant to scab, utilizing the gene [3]. In recent years, several apple cultivars containing have been introduced into commercial production from the PRI program, including "Dayton," "Prima," "Priscilla," and "Jonafree" [4]. Among the most promising introductions are "WineCrisp" [5], "CrimsonCrisp" [6], "Pixie Crunch" [7], and "GoldRush" [8]. In addition to the scab-resistant apple cultivars released by the PRI breeding program, several cultivars have also been released by breeding programs at Cornell University in NY, by Agriculture Canada, and by the National Institute of Agronomic Research in Angers, France, and Consorzio Italiano Vivaisti in Italy, and Breeding Initiative Niederelbe in Germany [9].Despite the introduction of several apple scab-resis...

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“…The members in the families show characteristic gene expression patterns and differential enzymatic properties (Minic and Jouanin, 2006;Tateishi, 2008). Since many ripening-related genes are induced by the ethylene signal, the effect of ethylene treatment on α-Larabinofuranosidase expression, particularly at the transcriptional level, has been extensively studied (Hayama et al, 2006;Mwaniki et al, 2007;Storch et al, 2015;Yoshioka et al, 2010). In many cases, however, it appears that the gene expression is not specifically regulated by ethylene or a ripening signal.…”

Section: Introductionmentioning

confidence: 99%

Opposite Accumulation Patterns of Two Glycoside Hydrolase Family 3 α-L-Arabinofuranosidase Proteins in Avocado Fruit during Ripening

et al. 2018

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Avocado fruit ripen with ethylene production after harvest and the flesh becomes soft and edible due to degradation of cell wall polysaccharides during ripening. α-L-Arabinofuranosidase is a hydrolytic enzyme known to digest arabinose-containing cell wall polysaccharides. It has been shown that its activity increased with fruit ripening. However, our previous study showed that an α-L-arabinofuranosidase gene (PaArf/Xyl3A) is expressed in the avocado fruit before ethylene production. In addition, the transcripts were detected in some organs in which the level of ethylene was extremely low. These results indicate that the gene expression is independent of ethylene. In the present study, we carried out immunoblot analyses of α-L-arabinofuranosidase at the protein level. Using a polyclonal antibody raised against Japanese pear α-L-arabinofuranosidase, two α-L-arabinofuranosidase proteins with molecular masses of 72 kDa and 68 kDa, presumably belonging to glycoside hydrolase family 3, were detected in ripening avocado fruit. The protein levels in ethylene or 1methylcyclopropene (1-MCP)-treated fruits were examined and the results indicated that the two proteins responded to ethylene in opposite ways; the 68 kDa protein showed a temporary accumulation, whereas the 72 kDa protein exhibited dissipation possibly caused by a loss of stability. The total enzyme activity of α-Larabinofuranosidase was elevated faster in the ethylene-treated fruit throughout ripening and was slower in the 1-MCP-treated fruit, suggesting the existence of another α-L-arabinofuranosidase, which did not crossreact with the antibody and was positively regulated by ethylene, in ripening avocado fruit.

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Ethylene-dependent regulation of an α-l-arabinofuranosidase is associated to firmness loss in ‘Gala’ apples under long term cold storage

Cited by 15 publications

References 35 publications

Identification of biomarkers associated to ‘Gala’ apples ripening and postharvest quality

Identification of biomarkers associated to ‘Gala’ apples ripening and postharvest quality

Influence of 1-Methylcyclopropene Treatment on Postharvest Quality of Four Scab (Venturia inaequalis)-Resistant Apple Cultivars

Opposite Accumulation Patterns of Two Glycoside Hydrolase Family 3 α-L-Arabinofuranosidase Proteins in Avocado Fruit during Ripening

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