Ethylene as a Factor Regulating the Growth of Pea Epicotyls Subjected to Physical Stress

Goeschl, J. D.; Rappaport, Lawrence; Pratt, Harlan K.

doi:10.1104/pp.41.5.877

Cited by 238 publications

(118 citation statements)

References 9 publications

(4 reference statements)

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“…We are not aware of reports on levels of ethylene in soils, but since the roots themselves produce the gas they are probably continuously exposed to it. Goeschl et al (11) have shown that ethylene production of peas is increas-ed by mechanical resistance to growth. It is therefore possible that roots in the soil produce even more ethylene than in the laboratory.…”

Section: Resultsmentioning

confidence: 99%

Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

Radin

Loomis

1969

Plant Physiol.

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Abstract. Ethylene is produced by cultured radish roots in amounts large enough to be physiologically important. When roots were grown in oontrolled atmospheres, applied ethylene was generally inhibitory to elongation, lateral root initiation, and cambial activity. 1 % CO2 similarly affected roots not given ethylene. In contrast, elongation and lateral root production of ethylene-treated roots were stimulated by 1 % COO. The results suggest that the oftenobserved stimulation of root growth by CO2 is due to an interaction with endogenous ethylene.

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Section: Resultsmentioning

confidence: 99%

Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

Radin

Loomis

1969

Plant Physiol.

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“…Production of ethylene by plant tissues is nearly a universal phenomenon (3), which is affected by many environmental factors, such as light (6,13) or physical and chemical stimuli (7,17) and also by such internal factors as growth regulators (3). Inasmuch as most physiological phenomena induced by ethylene are also regulated by other growth regulators, ethylene may play an important role in the regulation of cellular metabolisms which are Plant Physiol.…”

Section: Resultsmentioning

confidence: 99%

Induction of Peroxidase Activity by Ethylene in Sweet Potato

Imaseki

1970

Plant Physiol.

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“…Goeschl et al (6) postulated that the evolution of ethylene was in response to some physical stress and based their assumption on their observations that physical obstruction to elongation of the pea hypocotyl resulted in increased evolution of ethylene, followed by reduced length and increased diameter of both the internodes and the cells of the internodes. Burg and Burg (3) compared this phenomenon with ethylene evolution during germination of peas and assumed that physical stress is not a primary cause of ethylene production because, after radicle emergence, the ethylene production continued and remained constant while the epicotyl expanded.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Germination Rate of Aged Seeds by Ethylene

Takayanagi¹,

Harrington²

1971

Plant Physiol.

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Naturally and artificially aged seeds of rape, Brassica napus L., produced less ethylene than freshly harvested seed during the early stage of germination. With (9); it also breaks the dormancy of seed of various species (4,9,10,18,19). Stewart and Freebaim (17) showed that heat-treated lettuce seed which became insensitive to gibberellic acid germinated well subsequent to treatment with exogenous ethylene. Ruge (15) showed earlier that ethylene enhanced the percentage germination of aged oat seed. In the present paper, the pattern of ethylene production by rape seedlings in the early stage of germination and the stimulation of the germination of aged seeds by exogenous ethylene were studied. MATERIALS AND METHODSMaterials used in the experiments were the seeds of rape, Brassica napus L., variety Chisaya-natane, harvested in 1965 and seeds were sealed in tin cans and were put with the other seed at 3 C until the experiment was started.A germination test of the seeds was conducted with three replications (100 seeds X 3) on December 10, 1969. The blotter method at 25 C was used. The first count was on the 3rd day, and the final count was on the 7th day. The number of normal seedlings, abnormal seedlings, and slowly germinating seeds was counted.For observing ethylene production by seedlings and effects of exogenous ethylene on aged seeds, all experiments were conducted in Erlenmeyer flasks equipped with rubber serum stoppers. To prevent mold growth, the flasks, stoppers, and seeds were sterilized at the start of the experiment. Flasks containing filter paper or agar media were autoclaved at 15 psi for 20 min. The rubber stoppers were immersed in 95% ethanol and dried under sterile conditions. The seeds were treated with 95% ethanol for 5 sec, rinsed with sterile water, sterilized with 0.1% sodium hypochlorite for 30 min, and washed three times with sterile water. Seedlings were germinated and grown under continuous fluorescent light in a room maintained at 25 C. Samples of ethylene given off from seedlings in a flask were withdrawn with a 1-ml syringe through the rubber stopper. The amount of ethylene was determined by gas chromatography, using an alumina column maintained at 50 C and a flame ionization detector. Each experiment was replicated three times.The first two experiments were conducted to follow the trend of ethylene production during germination. In the first trial, the seeds were maintained in a closed system in 25-ml Erlenmeyer flasks containing two layers of wet filter paper. A 1-ml gas sample was withdrawn daily for 9 days from each flask for ethylene determination. In the second trial, the seeds were transferred aseptically to 125-ml Erlenmeyer flasks containing 25 ml of 1% agar. In contrast to the first trial, a 1-ml gas sample was taken every 6 or 12 hr for ethylene analysis, after which the gaseous content of the flask was flushed with fresh ethylene-free air. This trial was conducted for 120 hr.In the third experiment, ethylene was introduced into the flask containing aged seeds t...

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Ethylene as a Factor Regulating the Growth of Pea Epicotyls Subjected to Physical Stress

Cited by 238 publications

References 9 publications

Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

Ethylene and Carbon Dioxide in the Growth and Development of Cultured Radish Roots

Induction of Peroxidase Activity by Ethylene in Sweet Potato

Enhancement of Germination Rate of Aged Seeds by Ethylene

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