Ethyl Alcohol Extracts of Hizikia fusiforme Sensitize AGS Human Gastric Adenocarcinoma Cells to Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-Mediated Apoptosis

Kim, Tae Yeon; Jin, Cheng‐Yun; Kim, Gi-Young; Choi, Il‐Whan; Jeong, Yong Kee; Nam, Taek‐Jeong; Kim, Se-Kwon; Choi, Yung Hyun

doi:10.1089/jmf.2008.1114

Cited by 18 publications

(18 citation statements)

References 20 publications

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“…Recently the inhibitory properties of H. fusiforme on the production of pro-inflammatory mediators and ethanol- induced cytotoxicity have been demonstrated [2,16]. Additionally, we previously reported that EAHF sensitizes TRAIL-induced apoptosis and inhibits matrix metalloproteinase activity as well as tight junction in cancer cells [4,17]. Before now, there were no reports on whether EAHF itself induces apoptosis in cancer cells.…”

Section: Discussionmentioning

confidence: 98%

“…Previous research has reported that H. fusiforme has a variety of physiological activities, such as reactive oxygen species (ROS) scavenging and lipid oxidation inhibitory effects [3]. Recently, we demonstrated that the ethyl alcohol extract of H. fusiforme (EAHF) sensitizes cancer cells to apoptosis through the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), although no cytotoxicity was reported at low concentrations of EAHF itself [4].…”

Section: Introductionmentioning

confidence: 99%

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Ethyl Alcohol Extract of Hizikia fusiforme Induces Caspase-dependent Apoptosis in Human Leukemia U937 Cells by Generation of Reactive Oxygen Species

Kang¹,

Kang²,

Boo³

et al. 2011

Trop. J. Pharm Res

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Purpose: Hizikia fusiforme is renowned for the possession of anti-inflammatory and anti-oxidant properties. In this study, the role of the ethyl alcohol extract of H. fusiforme (EAHF) Results: EAHF suppressed the proliferation of U937 cells in a dose-dependent manner. This effect was closely related to the induction of apoptosis via the downregulation of IAP family members such as IAP-1, IAP-2 and XIAP, as well as Bcl-2 proteins. The results also showed that caspases play an essential role in EAHF-induced apoptosis by generating of reactive oxygen species (ROS). In addition, ROS scavenging by N-acetyl-L-cysteine (NAC) and glutathione (GSH) decreased EAHF-induced apoptosis via the suppression of caspase activity. Although EAHF induced the phosphorylation of mitogenactivated protein kinases (MAPKs), treatment with MAPK inhibitors did not affect EAHF-induced apoptosis. Conclusion: These results suggest that EAHF induces apoptosis in U937 cells via ROS-dependent caspase activation.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Ethyl Alcohol Extract of Hizikia fusiforme Induces Caspase-dependent Apoptosis in Human Leukemia U937 Cells by Generation of Reactive Oxygen Species

Kang¹,

Kang²,

Boo³

et al. 2011

Trop. J. Pharm Res

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show abstract

“…malignant cells. Ethyl alcohol extract of H. fusiforme treatment inhibits tumor metastasis and invasion in Hep3B human hepacocarcinoma cells [23] and sensitizes AGS human gastric adenocarcinoma cells to TNF-related apoptosis-inducing ligand-medicated apoptosis [9]. Another study demonstrated the immunostimulatory and anti-tumor effect of ginsan [24].…”

Section: Effects Of Hfae On the Production Of No And Cytokines In Rawmentioning

confidence: 94%

“…Polysaccharides isolated from plants and algae have been reported to enhance macrophage activation [6][7][8]. A variety of biological activities, including anti-cancer [9] and antiinflammatory [10] activity of H. fusiforme have been reported, but little is known about the molecular mechanism of H. fusiforme as an immunostimulator.…”

Section: Introductionmentioning

confidence: 99%

Immunostimulatory effect by aqueous extract of Hizikia fusiforme in RAW 264.7 macrophage and whole spleen cells

Yoon

Lee²,

Park³

et al. 2011

Biotechnol Bioproc E

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Hizikia fusiforme is a commonly used food that possesses potent anti-bacterial, anti-fungal, and antiinflammatory activities. The immunostimulatory activities of aqueous extract of Hizikia fusiforme (HFAE) in RAW 264.7 macrophages and whole spleen cells were investigated. HFAE activated RAW 264.7 macrophages to produce cytokines such as nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in a dose-dependent manner. In addition, HFAE induced the mRNA expression of TNF-α, IL-1β, and IL-6 in RAW 264.7 macrophages. Moreover, HFAE stimulated proliferation of whole spleen cells and reference mitogen. Taken together, the results demonstrate that HFAE potently activates the immune function by regulating NO, TNF-α, IL-1β, and IL-6 in RAW 264.7 macrophage and promoting spleen cell proliferation.

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“…In this study, [16] Ethyl alcohol extract of H. fusiforme was prepared as previously described [12]. Briefly, dried H. fusiforme were extracted in 80% ethyl alcohol at a ratio of 10 ml/g for 24 hr in a refrigerator at 4°C.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Cell Invasion by Ethyl Alcohol Extracts of Hizikia fusiforme in AGS Human Gastric Adenocarcinoma Cells

Choi

2010

Journal of Life Science

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In this study, we investigated the effects of ethyl alcohol extracts of Hizikia fusiforme (EHF) on the correlation between tightening of tight junctions (TJs) and anti-invasive activity in human gastric adenocarcinoma AGS cells. Inhibitory effects of EHF on cell proliferation, motility, and invasiveness were found to be associated with increased tightness of the TJs, which was demonstrated by an increase in transepithelial electrical resistance. Activities of matrix metalloprotease (MMP)-2 and -9 in AGS cells were dose-dependently inhibited by treatment with EHF, and this was also correlated with a decrease in expression of their mRNA and proteins; however, tissue inhibitor of metalloproteinase (TIMP)-1 and -2 mRNA levels were increased. Additionally, immunoblotting results indicated that EHF repressed the levels of claudin proteins (claudin-1, -3, and -4), major components of TJs that play key roles in control and selectivity of paracellular transport. Furthermore, EHF decreased expression of insulin such as growth factor-1 receptor proteins, while concurrently increasing that of thrombospondin-1 and E-cadherin. In conclusion, these results suggest that EHF treatment may inhibit tumor cell motility and invasion, and therefore act as a dietary source to decrease the risk of cancer metastasis.

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Ethyl Alcohol Extracts of Hizikia fusiforme Sensitize AGS Human Gastric Adenocarcinoma Cells to Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-Mediated Apoptosis

Cited by 18 publications

References 20 publications

Ethyl Alcohol Extract of <i>Hizikia fusiforme</i> Induces Caspase-dependent Apoptosis in Human Leukemia U937 Cells by Generation of Reactive Oxygen Species

Ethyl Alcohol Extract of <i>Hizikia fusiforme</i> Induces Caspase-dependent Apoptosis in Human Leukemia U937 Cells by Generation of Reactive Oxygen Species

Immunostimulatory effect by aqueous extract of Hizikia fusiforme in RAW 264.7 macrophage and whole spleen cells

Inhibition of Cell Invasion by Ethyl Alcohol Extracts of Hizikia fusiforme in AGS Human Gastric Adenocarcinoma Cells

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