The present study is aimed to screen the phytochemicals analysis and elements content of Datura innoxia seeds and leaves. The aqueous and methanol extracts were carried out by using the maceration method and soxhlet apparatus, respectively. Results of the study of Phytochemical screening revealed the presence of a high concentration of alkaloids, flavonoids, tannins, steroids and triterpines, and low concentration of saponin and coummarins. The elements in the leaves and seeds (K, Ca, S, Si, Cl, Fe, Al, P, Mg, Ti, Mn, Zn, Sr, Cu, V, Br, and Zr) were determined by energy-dispersive X-ray fluorescence (EDXRF) spectroscopy. K content was the highest in seeds (5.469 ± 0.021%), Ca and S the highest in leaves (2.461 ± 0.019 %, 1.254 ± 0.022 %, respectively). The elements Ti, Mn, Sr, V, Br, and Zr were detected in the leaves with range concentration 0.062-0.002 %. The elements Si, Cl, Fe, Al, P, Mg, and Zn concentration in seeds varied from 0.002 to 0.942% and in leaves varied from 0.014 to 0.346%. The concentration of these elements did not exceed the standard of dangerous toxic levels.