Ethnobotany: A Living Science for Alleviating Human Suffering

Qureshi, Rahmatullah; Ghazanfar, Shahina A.; Obied, Hassan K.; Vasileva, Viliana; Tariq, Mohammad

doi:10.1155/2016/9641692

Cited by 26 publications

(21 citation statements)

References 6 publications

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“…19 Scientists are targeting plant extracts to treat these complicated diseases, hypothesizing on their long utilization history by traditional communities. 6 , 20 According to the WHO report, the drug market for plant-derived compounds was approximately 100 million USD in 2007, and it is expected to reach 5 trillion USD by the year 2050. 5 A remarkable number of anticancer drugs have been made from medicinal plants and are currently in clinical use, based on ethnobotanical as well as ethnopharmacological exploration.…”

Section: Discussionmentioning

confidence: 99%

In vitro anticancer activities of Withania coagulans against HeLa, MCF-7, RD, RG2, and INS-1 cancer cells and phytochemical analysis

Maqsood

Qureshi

Ikram

et al. 2018

Integrative Medicine Research

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BackgroundThe Pakistani Salt Range has a rich floral diversity including Withania coagulans from the Solanaceae family.MethodsThe crude methanolic extracts of the root, leaf, leaf stalk, and fruit of this plant were screened for their cytotoxic activity against human (HeLa, MCF-7, RD) and rat (RG2 and INS-1) cancer cell lines at 20 μg/mL and compared to methotrexate. The IC50 values indicated that leaf stalk and fruit extracts exert an 80% or higher cytotoxic activity against all cell lines at 24 hours.ResultsThe leaf stalk extract showed the highest cytotoxic efficacy against all tested cell lines, with IC50 values ranging from 0.96 ± 0.01 μg/mL to 4.73 ± 0.05 μg/mL followed by the fruit extract with IC50 values of 0.69 ± 0.01–6.69 ± 0.06 μg/mL after 48–72 hours incubation. The leaf stalk and seed extracts were analyzed for polyphenols and flavonoids using RP-HPLC. The total flavonoid content (TFC) was calculated for all tested samples, and the highest TFC was recorded for the root extract (394.34 ± 1.26 μg/g). The total phenolic content (TPC) was found in the seed extract (307.86 ± 9.42 μg/g) of W. coagulans. The highest contents of myricetin (358.46 ± 2.91 μg/g) were noted in the leaf extract, and highest quercetin was recorded in the seed extract (21.43 ± 0.13 μg/g). The highest gallic acid concentration (83.62 ± 0.71 μg/g) was recorded in leaf stalk extract and p-hydroxybenzoic acid in the seed extract (157.46 ± 1.43 μg/g).ConclusionThe present study gives a scientific insight and comparative analysis of various plant parts in this medicinally important plant species from the Salt Range of Pakistan against both human and rat cancer cells.

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Section: Discussionmentioning

confidence: 99%

In vitro anticancer activities of Withania coagulans against HeLa, MCF-7, RD, RG2, and INS-1 cancer cells and phytochemical analysis

Maqsood

Qureshi

Ikram

et al. 2018

Integrative Medicine Research

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“…Herbal medicine is additionally prevalent in under-developed and developing countries where infectious diseases are common, compounded by poverty, poor hygiene and sanitation, and inaccessibility or high cost of good healthcare [ 25 , 26 ]. In fact, it is estimated that 80% of the under-developed and developing world population rely on plant-derived remedies for alleviating various ailments [ 19 , 27 , 28 , 29 , 30 , 31 ]. This fact is reinforced by the findings that plants are a rich source of bioactive molecules as efficacious as synthetic pharmaceutics or that can either be chemically modified to enhance their potency or act as templates for new pharmacophores [ 19 , 32 , 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity, phytochemical characterization and gas chromatography-mass spectrometry analysis of Aspilia pluriseta Schweinf. extracts

Njeru

Muema

2020

Heliyon

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Aspilia pluriseta is associated with various bioactivities, although with limited scientific justification. In this study, we evaluated the antimicrobial activity, and characterized the phytochemicals of root extracts of A. pluriseta aimed at validating its therapeutic potential. We used BACTEC MGIT™ 960 system to test for antitubercular activity, disc-diffusion together with the microdilution method to evaluate antimicrobial activities and qualitative phytochemical tests together with gas chromatography-mass spectrometry (GC-MS) analysis to determine the phytochemicals that associated with A. pluriseta extracts activity. We show that methanolic crude extract (at 1 g/mL) had high Mycobacterium tuberculosis (MTB) inhibitory activity (0 growth unit) and considerable potency against Escherichia coli (11.7 mm), Staphylococcus aureus (9.0 mm), and Candida albicans (7.7 mm). All the extract fractions exerted remarkable antimycobacterial activities with minimum inhibitory activity of between 6.26 – 25 μg/mL. The highest antimicrobial activity of petroleum ether and dichloromethane fraction was against E. coli at inhibition zone diameters of 8.3 mm, and 8.0 mm, respectively, while ethyl acetate fraction was against S. aureus with an inhibition zone of 8.7 mm. Methanolic fraction exhibited broad-spectrum activity against 87.5% of the tested microbes (inhibition zones 6.3–8.3 mm). Furthermore, we qualitatively detected terpenoids, alkaloids, and phenolics such as flavonoids, and anthraquinones in extract fractions. GC-MS analysis detected an abundance of fatty acid esters, 2-hydroxy-1-(hydroxymethyl) ethyl ester-hexadecanoic acid, and 2,3-dihydroxy propyl ester-octadecanoic acid and four alkanes. Taken together, we show that A. pluriseta extract fractions (especially ethyl acetate and methanolic fractions) have strong selective antitubercular activity, and thus, we scientifically validate the use of A. pluriseta as a potential source for the discovery of novel antitubercular agents.

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“…However, many probiotic bacteria are unable to survive under adverse temperature and acidity environments, and thus stabilization techniques should be applied. Studies aiming for the valorization of plants and lactic acid bacteria with probiotic and prebiotic potential as sources of innovative natural products and/or molecules for both traditional and modern medicine are re-emerging [13]. However, the use of combinations of biologically active compounds and viable cells, such as anthocyanins and lactic bacteria, in the same microencapsulated formula for the purpose of the cumulative exploitation of benefits, are not commonly reported.…”

Section: Introductionmentioning

confidence: 99%

Co-Microencapsulation of Anthocyanins from Cornelian Cherry Fruits and Lactic Acid Bacteria in Biopolymeric Matrices by Freeze-Drying: Evidences on Functional Properties and Applications in Food

et al. 2020

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Cornus mas was used in this study as a rich source of health-promoting bioactives. The cornelian cherries were used to extract the polyphenols and anthocyanins. The chromatographic profile of the Cornus mas fruit extract revealed the presence of several anthocyanins, mainly delphinidin, cyanidin and pelargonidin glycosides. The extract was co-microencapsulated with Lactobacillus casei ssp. paracasei in a unique combination of whey protein isolates, inulin and chitosan by freeze-drying, with an encapsulation efficiency of 89.16 ± 1.23% for anthocyanins and 80.33 ± 0.44% for lactic acid bacteria. The pink-red colored powder showed a total anthocyanins content of 19.86 ± 1.18 mg cyanidin-3-glucoside/g dry weight (DW), yielding an antioxidant activity of 54.43 ± 0.73 mMol Trolox/g DW. The viable cells were 9.39 × 109 colony forming units (CFU)/g DW. The confocal microscopy analysis revealed the microencapsulated powder as a complex one, with several large formations containing smaller aggregates, consisting of the lactic acid bacteria cells, the cornelian cherries’ bioactive compounds and the biopolymers. The powder was tested for stability over 90 days, showing a decrease of 50% in anthocyanins and 37% in flavonoids content, with no significant changes in antioxidant activity and CFU. The powder showed a significant inhibitory effect against the α-amylase of 89.72 ± 1.35% and of 24.13 ± 0.01% for α-glucosidase. In vitro digestibility studies showed a significant release of anthocyanins in gastric juice, followed by a decrease in intestinal simulated conditions. The functional properties of the powder were tested by addition into a yogurt, highlighting a higher and more stable antioxidant activity at storage when compared to the control.

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Ethnobotany: A Living Science for Alleviating Human Suffering

Cited by 26 publications

References 6 publications

In vitro anticancer activities of Withania coagulans against HeLa, MCF-7, RD, RG2, and INS-1 cancer cells and phytochemical analysis

In vitro anticancer activities of Withania coagulans against HeLa, MCF-7, RD, RG2, and INS-1 cancer cells and phytochemical analysis

Antimicrobial activity, phytochemical characterization and gas chromatography-mass spectrometry analysis of Aspilia pluriseta Schweinf. extracts

Co-Microencapsulation of Anthocyanins from Cornelian Cherry Fruits and Lactic Acid Bacteria in Biopolymeric Matrices by Freeze-Drying: Evidences on Functional Properties and Applications in Food

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