Ethnic variation in red cell glutathione peroxidase activity

Beutler, E; Matsumoto, F

doi:10.1182/blood.v46.1.103.bloodjournal461103

Cited by 8 publications

(8 citation statements)

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“…In addition to the difference in activites found among various ethnic groups (Beutler & Matsumoto, 1975), decreased levels of GP have been described in several clinical conditions. These include prolonged parenteral nutrition (Fleming et al, 1982), cancer, Keshan's disease (Chen et al, 1980).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant enzymes in lymphocytes from normal subjects and patients with chronic lymphocytic leukaemia: increased glutathione peroxidase activity in CLL B lymphocytes

et al. 1989

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The activities of several enzymes that protect against oxidative injury were determined in blood lymphocytes from patients with B chronic lymphocytic leukaemia (CLL) and from normal subjects. Similar glutathione reductase (GR), catalase and glucose-6-phosphate dehydrogenase (G6PD) activities were found in normal and CLL lymphocytes. Higher glutathione peroxidase (GP) activity was found in CLL lymphocytes. This activity in CLL B lymphocytes was 2-fold higher than that of normal B lymphocytes, and 3-fold higher than that of T lymphocytes from either source. Several disease processes have been associated with decreased glutathione peroxidase activity. Our finding with CLL B lymphocytes is believed to be the first example of an increased GP activity in a disease. It may reflect either the expansion of a rare type of B cell population or be an expression of the malignant process.

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Section: Discussionmentioning

confidence: 99%

Antioxidant enzymes in lymphocytes from normal subjects and patients with chronic lymphocytic leukaemia: increased glutathione peroxidase activity in CLL B lymphocytes

et al. 1989

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“…Levels of ATP, reactive oxygen species (ROS), glutathione (GSH), malondialdehyde (MDA), and mitochondrial cytochrome oxidase and superoxide dismutase (SOD) activity in liver homogenates were, respectively, determined by using commercial kits (Nanjing Jiancheng Biotech. Ltd. Co., Nanjing, China), where ATP level was assayed by luciferase-fluorescein bioluminescent method ( Sevin et al, 1988 ), ROS was detected by Fenton reaction and Griess reagent ( Lin et al, 2014 ), GSH was determined by the spectrophotometric method based on the use of Ellman’s reagent ( Buege and Aust, 1978 ), the MDA level was assayed by monitoring thiobarbituric acid reactive substance formation ( Beutler, 1975 ; Fu et al, 2006 ), mitochondrial cytochrome oxidase and SOD activity were measured by using resazurin method and pyrogallol autoxidation method, respectively ( Marklund and Marklund, 1974 ; Wang et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Mitotherapy for Fatty Liver by Intravenous Administration of Exogenous Mitochondria in Male Mice

et al. 2017

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Mitochondrial dysfunction is a major and common mechanism in developing non-alcoholic fatty liver disease (NAFLD). Replacement of dysfunctional mitochondria by functional exogenous mitochondria may attenuate intrahepatic excessive lipid and recover hepatocyte function. However, no data shows that mitochondria can be systemically administrated to animals to date. Here we suggest that mitochondria isolated from hepatoma cells are used as a mitotherapy agent to treat mouse fatty liver induced by high-fat diets. When the mitochondria were intravenously injected into the mice, serum aminotransferase activity and cholesterol level decreased in a dose-dependent manner. Also, the mitotherapy reduced lipid accumulation and oxidation injury of the fatty liver mice, improved energy production, and consequently restored hepatocyte function. The mitotherapy strategy offers a new potential therapeutic approach for treating NAFLD.

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“…Reduced GSH was determined according to Beutler’s method using Ellman’s reagent (Beutler 1975). The procedure is based on the reduction of Ellman’s reagent by –SH groups to form 5,5′-dithiobis (2-nitrobenzoic acid) with an intense yellow color, measured spectrophotometrically at 412 nm using a Shimadzu Spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Combined Effects of Vitamin C, Vitamin E, and Sodium Selenate Supplementation on Absolute Ethanol-Induced Injury in Various Organs of Rats

et al. 2007

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In this study, the effect of combination of vitamin C (ascorbic acid), vitamin E (alpha -tocopherol), and selenium (sodium selenate) on ethanol-induced liver and intestine injury in rats was investigated. The ethanol-induced injury was produced by the administration of 1 ml of absolute ethanol to each rats. Animals received vitamin C (250 mg/kg), vitamin E (250 mg/kg), and sodium selenate (Se) (0.5 mg/kg) for 3 days; 1 h after the final antioxidant administration, they were sacrificed. Lipid peroxidation and glutathione levels, catalase (CAT), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and glutathione peroxidase (GP(x)) activities were determined in liver and intestine tissues. Myeloperoxidase (MPO), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT) were determined in liver tissue. Also, CAT activity, urea, creatinine, uric acid, and total lipid levels were determined in serum samples. In the ethanol group, serum urea, creatinine, uric acid, and total lipid levels; liver and intestine LDH; liver MPO, AST, ALP, ALT, and GGT activities; and liver and intestine LPO levels increased, whereas serum CAT activity, liver and intestine GSH levels, and CAT, SOD, and GP(x) activities decreased. On the other hand, treatment with vitamin C, vitamin E, and Se reversed these effects. As a result of these findings, we can say that the combination of vitamin C, vitamin E, and selenium has a protective effect on ethanol-induced changes in lipid peroxidation, glutathione levels, and antioxidant enzyme activities in liver and intestine tissues, and in some serum parameters of rats.

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Ethnic variation in red cell glutathione peroxidase activity

Abstract: Glutathioneperoxidase activity was measured in blood and cultured fibroblasts from healthy persons of several different

Cited by 8 publications

References 0 publications

Antioxidant enzymes in lymphocytes from normal subjects and patients with chronic lymphocytic leukaemia: increased glutathione peroxidase activity in CLL B lymphocytes

Antioxidant enzymes in lymphocytes from normal subjects and patients with chronic lymphocytic leukaemia: increased glutathione peroxidase activity in CLL B lymphocytes

Mitotherapy for Fatty Liver by Intravenous Administration of Exogenous Mitochondria in Male Mice

Combined Effects of Vitamin C, Vitamin E, and Sodium Selenate Supplementation on Absolute Ethanol-Induced Injury in Various Organs of Rats

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