The rapid development of the internet influences information availability. It makes easier for someone to do the plagiarism of a work. The rise of information available online makes the habit of copy-paste without mentioning the reference to become easy so that scientific work unwittingly becomes the result of plagiarism from other scientific works. Plagiarism prevention efforts are envolving in various sector. Designing and developing plagiarism checker applications is the purpose of this paper. Specifically by knowing the percentage of similarity between the original document and the test document. This research using Winnowing algorithm because it can detect plagiarism in documents up to subsection of the document. This research using three validates consisting of computational mathematicians, software engineering experts, and users to test the application feasibility. The experiment uses several scenarios and the result of effectiveness evaluation yields 82% sensitivity, 100% specificity, and 91% accuracy. The implemented system works effectively so the system can be used to detect document plagiarism.