Ethanolic Extract from Pteris wallichiana Alleviates DSS-Induced Intestinal Inflammation and Intestinal Barrier Dysfunction by Inhibiting the TLR4/NF-κB Pathway and Regulating Tight Junction Proteins

Abstract

The aim of the research was to determine the protective effect and mechanism of Pteris wallichiana J. Agardh extract (PWE) on DSS-induced ulcerative colitis (UC) in mice. In this research, PWE is rich in flavonoids and diterpenoids by UPLC-MS/MS analysis. In LPS-induced RAW264.7 cells, PWE reduced the productions of inflammatory factors (i.e., NO, TNF-α, IL-6, and IL-1β). In DSS-induced UC in mice, PWE improved disease activity index (DAI) score, attenuated oxidative stress by decreasing MPO and MDA activities and activating GSH and SOD levels, and inhibited TNF-α, IL-6, and IL-1β expressions in the colonic tissues. PWE also improved the intestinal barrier by upregulating the expressions of tight junction proteins, including occludin and ZO-1. Moreover, PWE extract alleviated intestinal inflammation by suppressing the TLR4/MyD88/NF-κB signaling pathway. Conclusion: PWE can alleviate DSS-induced UC in mice by increasing the expressions of intestinal tight junction proteins and inhibiting the TLR4/NF-κB inflammatory pathway.

Dexmedetomidine alleviates intestinal barrier dysfunction and inflammatory response in mice via suppressing TLR4/MyD88/NF-κB signaling in an experimental model of ulcerative colitis

“…According to a previous study, TLR4/MyD88/NF-κB signaling is associated with intestinal inflammation in UC [52,53], and DEX was shown to inhibit TLR4/MyD88/NF-κB signaling in septic acute kidney injury [54]. Therefore, whether DEX can regulate this pathway in the experimental UC model was investigated in our study.…”

Naringin mitigates LPS-induced intestinal barrier injury in mice

“…24,25 ZO-1, occludin, JAM2 and claudin-1 are intestinal epithelial tight junction proteins, which play an important role in maintaining the integrity of intestinal epithelia. 26 MUC2, distributed in small intestine and large intestine, is the main component of the intestinal mucus layer, and the contents of MUC2 can strengthen the intestinal barrier. 27 In this study, our data indicated that LPS significantly increased the DAO activity and the D-LA content in serum and decreased the mRNA expression of tight junction protein related genes ZO-1, occludin, claudin-1, MUC2 and JAM2 in jejunum of mice.…”

Bioactive Compounds in Moringa oleifera: Mechanisms of Action, Focus on Their Anti-Inflammatory Properties

“…At 0.3%, kaempferol ( 10 ) decreased the plasma leukotriene B4 [LTB(4)] level in all treated animals, while NO and PGE2 contents decreased significantly [ 110 ]. Kaempferol ( 10 ) also suppressed MPO activity in the colon mucosa [ 114 ]. Additionally, in kaempferol ( 10 ) pre-treated animals, the quantity of TFF3 (a marker of goblet cell function) mRNA was upregulated, indicating its usefulness [ 110 , 115 ].…”