Ethanol stress responses of Kluyveromyces marxianus CCT 7735 revealed by proteomic and metabolomic analyses

Alvim, Mariana Caroline Tocantins; Vital, Camilo Elber; Barros, Edvaldo; Vieira, Nívea Moreira; Silveira, Fernando A.; Balbino, Thércia Rocha; Diniz, Raphael Hermano Santos; Brito, Amanda Fernandes; Bazzolli, Denise Mara Soares; Ramos, Humberto Josué de Oliveira; Silveira, Wendel Batista da

doi:10.1007/s10482-018-01214-y

Cited by 20 publications

(18 citation statements)

References 60 publications

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“…Based on the type of compound and its location in the metabolic pathway, these metabolites are classified into different classes, such as glycometabolism, amino acids and carbohydrates. By metabolomics analysis of the adhesive activity of L. plantarum ATCC 14917 under initial acid and alkali stress (Wang et al, 2018a(Wang et al, ,2018b, some similar results were obtained; that is, under certain stress conditions, the intracellular metabolites, especially amino acids, fatty acids and carbohydrates, would change to some extent.…”

Section: Changes In Intracellular Metabolites In Ethanol-treated Cellsmentioning

confidence: 78%

“…Trehalose has a protective effect on microorganisms under environmental stress and helps to maintain the normal osmotic pressure of cells. Because of its high water holding capacity, it can stabilise the structure of macromolecular substances such as biofilm, protein and nucleic acid in cells (Wang et al, 2018a(Wang et al, ,2018b. A large body of evidence indicates that the accumulation of intracellular trehalose may be beneficial for the survival of cells under environmental stress (Wang et al, 2014;Divate et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Metabolomics analysis of growth inhibition of Lactobacillus plantarum under ethanol stress

Chen

Wang

Jin

et al. 2020

Int J of Food Sci Tech

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This experiment investigated the changes in morphology, cell membrane permeability and intracellular metabolites of Lactobacillus plantarum (L. plantarum) under different concentrations of ethanol stress. It was found that when treated with ethanol at the concentration of 8% (v/v), L. plantarum mortality was close to 60%, and the surface of cell was rough and collapsed, and the cell debris is obvious. The extracellular β-galactosidase activity increased to 1.24 and 1.73 times higher as compared to the control group after 6% (v/v) and 8% (v/v) ethanol stress, respectively. The inhibition of bacterial growth was positively correlated with its concentration. Ethanol stress can cause changes in cell morphology and membrane permeability of L. plantarum, causing physiological damage to cells. Metabolomics analysis revealed that the ethanol stress led to the inhibition of primary metabolic pathways through the suppression of the tricarboxylic acid cycle and glycolysis. It is therefore proposed that the inhibition effect of ethanol on L. plantarum is achieved by inducing intracellular metabolic imbalance via disruption of cell membrane functions. Importance Lactobacillus plantarum is often subjected to ethanol stress during food processing, production and storage, thus inhibiting the growth of bacteria. However, the detailed inhibition mechanism of ethanol on L. plantarum has not been deeply studied on metabolomics analysis. By measuring the changes of intracellular metabolites under ethanol stress, we found that ethanol destroyed the function of the membrane by changing the membrane structure, which hindered the carbon metabolism in the cell centre and reduced the vitality of bacteria. The results made a contribution for improvement of the target strain physiological and the strain industrial utility under ethanol stress.

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Section: Changes In Intracellular Metabolites In Ethanol-treated Cellsmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Metabolomics analysis of growth inhibition of Lactobacillus plantarum under ethanol stress

Chen

Wang

Jin

et al. 2020

Int J of Food Sci Tech

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“…The results were consistent with previous findings that ergosterol content was involved in stress tolerance of yeast cells. Indeed, Bhattacharya et al () found that precise regulation of ergosterol genes was essential for cellular homeostasis when yeast cells faced with multiple stresses, and Alvim et al () reported that ergosterol was related to ethanol stress tolerance.…”

Section: Resultsmentioning

confidence: 99%

Cellular mechanism for the improvement of multiple stress tolerance in brewer's yeast by potassium ion supplementation

Yang

Brennan

et al. 2020

Int J of Food Sci Tech

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The ethanol fermentation efficiency was affected by multiple stress tolerance of yeast during brewing and bioethanol industry. The effect of KCl on the multiple stress tolerance of yeast cells was examined. Results showed that KCl addition significantly enhanced the tolerance of yeast cells to osmotic and ethanol stress, which correlated with the decreased membrane permeability, the increased intracellular ergosterol and ATP content, and the improved activity of complex II and complex III in yeast cells. Biomass and viability of yeast cells under osmotic and ethanol stress were increased significantly by KCl addition. Supplementation of 4 and 10 g L À1 KCl exhibited the best promotion activity for yeast cells present in medium with 500 g L À1 sucrose and 10% (v v À1 ) ethanol, respectively. These results suggested that exogenous potassium addition might be an effective strategy to improve yeast tolerance and fermentation efficiency during industrial very-high-gravity (VHG) fermentation.

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“…In the work of Fu et al [19], in contrast, at high temperature (45 o C), genes related to lipid metabolism of the plasma membrane were upregulated and K. marxianus DMKU3-1042 also produced more ergosterol than it did in the normal condition. Differences in the expression patterns of lipid metabolism-encoding genes and/or ergosterol profiles between the studies of Diniz et al [15], Wang et al [18], Alvim et al [17], and Fu et al [19] might be explained by the differences in K. marxianus strains used in these experiments (e.g., CCT 7735, YHJ010, CCT 7735, and DMKU3-1042, respectively) since they used the same method [20] to measure ergosterol content.…”

Section: Introductionmentioning

confidence: 90%

“…The downregulation of genes involved in central carbon metabolism are consensus between ethanol stress [15,17], high temperature [19], and mixed inhibitors stress [18] (Table 1). In 1995, Piper stated that many changes induced by ethanol stress were similar to those triggered by heat stress and the synergistic effects of heat and ethanol stresses were recorded [21].…”

Section: Introductionmentioning

confidence: 99%

Kluyveromyces marxianus: Current State of Omics Studies, Strain Improvement Strategy and Potential Industrial Implementation

Ha-Tran¹,

Nguyen²

2020

Preprint

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Bioethanol has been considered as an excellent alternative to fossil fuels since it importantly contributes to the reduced consumption of the crude oil and to the alleviation of environmental pollution [1]. Up to now, the baker yeast Saccharomyces cerevisiae is the most common eukaryotic microorganism used in ethanol production. The inability of S. cerevisiae to grow on pentoses, however, hinders its effective growth on plant biomass hydrolysates, which contain large amounts of C5 and C12 sugars. The industrial-scale bioprocessing requires high temperature bioreactors, diverse carbon sources, and the high titer production of volatile compounds [2]. These criteria indicate that the search for alternative microbes possessing useful traits that meet the required standards of bioethanol production is necessary. Compared to other yeasts, Kluyveromyces marxianus has several advantages over the others, e.g. it could grow on a broad spectrum of substrates (C5, C6 and C12 sugars) [3], tolerate to high temperature, toxin [4,5] and a wide range of pH values [6], and produce volatile short-chain ester [2]. K. marxianus also shows a high ethanol production rate at high temperature and is a Crabtree-negative species [7]. These attributes make K. marxianus a promise as an industrial host for the biosynthesis of biofuels and other valuable chemicals.

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Ethanol stress responses of Kluyveromyces marxianus CCT 7735 revealed by proteomic and metabolomic analyses

Cited by 20 publications

References 60 publications

Metabolomics analysis of growth inhibition of Lactobacillus plantarum under ethanol stress

Metabolomics analysis of growth inhibition of Lactobacillus plantarum under ethanol stress

Cellular mechanism for the improvement of multiple stress tolerance in brewer's yeast by potassium ion supplementation

Kluyveromyces marxianus: Current State of Omics Studies, Strain Improvement Strategy and Potential Industrial Implementation

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