Ethanol production in a microporous hollow‐fiber‐based extractive fermentor with immobilized yeast

Abstract

Microporous-membrane-based extractive product recovery in product-inhibited fermentations allows in situ recovery of inhibitory products in a nondispersive fashion. A tubular bioreactor with continuous strands of hydrophobic microporous hollow fibers having extracting solvent flowing in fiber lumen was utilized for yeast fermentation of glucose to ethanol. Yeast was effectively immobilized on the shell side in small lengths of chopped microporous hyrophilic hollow fibers. The beneficial effects of in situ dispersion-free solvent ex (oleyl alcohol and dibutyl phthalate) were demonstrated for a 300 g/L glucose substrate feed. Outlet glucose concentration dropped drastically from 123 to 41 g/L as solvent/ substrate flow ratio was increased from 0 to 3 at 9 mL/h of substrate flow rate with oleyl alcohol as extracting solvent. The significant productivity increase with in situ solvent extraction became more evident as solvent/ substrate flow ratio increased. A model of the locally integrated extractive bioreactor describes the observed fermentor performance quite well.

A study of polynucleotide phosphorylase production by Escherichia coli in a hollow fibre reactor

“…The asymmetric hollow fibre membrane (Tianjin Institute for Membrane, China) was made of polysulfone, with inner and outer diameters of 1.0 and 1.2 mm and a molecular cut-off of 100,000 Da (Kang et al 1990; sterile distilled water, was connected to the fermentor (Bibal et al 1991;Uribelarrea et al 1997).…”

Polymeric Membranes for Integrated Reaction and Separation

“…In general, only ultrafiltration (UF) or microfiltration (MF)-based processes have been reported and little is known on the application of reverse osmosis (RO) or nanofiltration (NF) in membrane bioreactors. Additionally, membrane contactor systems have been developed, based on microporous polyolefin or teflon membranes [52][53][54][55].…”

Ethanol fermentation coupled with complete cell recycle pervaporation system: Dependence of glucose concentration

“…several possible alternatives have been proposed: elimination of product inhibition by continuous product removal by either extraction, vacuum or membrane distillation and by pervaporation (Shabtai et al , 1991;Cysewski anti Wilke, 1977;Mori and Inaba, 1990;Udriot et al , 1989;Kang et al , 1990). several possible alternatives have been proposed: elimination of product inhibition by continuous product removal by either extraction, vacuum or membrane distillation and by pervaporation (Shabtai et al , 1991;Cysewski anti Wilke, 1977;Mori and Inaba, 1990;Udriot et al , 1989;Kang et al , 1990).…”