Ethanol production from henequen (Agave fourcroydes Lem.) juice and molasses by a mixture of two yeasts

Cáceres-Farfan, Mirbella; Lappe, Patricia; Larqué-Saavedra, Alfonso; Magdub-Méndez, Abdo; Barahona-Pérez, Luis Felipe

doi:10.1016/j.biortech.2008.04.063

Cited by 35 publications

(23 citation statements)

References 15 publications

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“…Similar reports of ethanol production by Bacillus strains from Agave biomass have been found by other researchers [4]. Other microorganisms such as yeasts could also produce ethanol from Agave biomass fermentation [24][25][26].…”

Section: Production Of Ethanol By Degradation Of Agave Biomasssupporting

confidence: 69%

Two Bacillus Species Isolated from Rotting Wood Samples are Good Candidates for the Production of Bioethanol using Agave Biomass

Paudel¹,

Qin

2015

J Microb Biochem Technol

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The biorefining of crop and plant organic matter represents a promising route to produce renewable fuels and bioproducts. The high contents of cellulose and hemicellulose make Agave americana an ideal candidate to produce value-added products. In this study, two different cellulase producing bacteria, isolated from rotting wood samples, were incubated and cultivated to examine their ability to decompose agave and produce ethanol. The results showed the transparent zones called halos on the plates containing Agave as the sole carbon source after iodine staining for these two isolates and positive control Cellulomonas xylanilytica; while, no halo was observed for negative control: Escherichia coli BL21. The Bacillus species K1 and A0 displayed hydrolysis ability greater than that of positive control based on halo diameter. Moreover, the quantitative ability to decompose agave was studied for the same two bacterial strains using minimal salt media containing 5% agave biomass. Dinitrosalicylic acid (DNS) method was used to detect cellulase and reducing sugars. Ethanol was detected by using micro-dichromate method. The results showed that strain K1 produced 0.435 g ethanol/g Agave biomass and the isolate A0 produced 0.397 g/g ethanol on the 4 th day of incubation.

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Section: Production Of Ethanol By Degradation Of Agave Biomasssupporting

confidence: 69%

Two Bacillus Species Isolated from Rotting Wood Samples are Good Candidates for the Production of Bioethanol using Agave Biomass

Paudel¹,

Qin

2015

J Microb Biochem Technol

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“…However, the assay kit did not overcome the interference by glucose and glycerol. Although GC can be used for the determination of bioethanol, the dichromate oxidation method is still used after distillation of the culture broth [5].…”

Section: Discussionmentioning

confidence: 99%

Measurement of ethanol concentration using solvent extraction and dichromate oxidation and its application to bioethanol production process

Seo¹,

Kim

Lee

et al. 2008

J Ind Microbiol Biotechnol

107

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A method for measuring the ethanol concentration in a yeast culture broth was developed using both microtubes and a 96-deepwell microplate. The strategy involved first the solvent extraction of ethanol from the yeast culture broth and measurements of the ethanol concentration using the dichromate oxidation method. Particular focus was made on selecting the extraction solvent as well as determining the measurable range of ethanol concentrations using this solvent extraction-dichromate oxidation method. This method was developed as an assay format in 2.0-ml microtubes and 1.2-ml 96-deepwell microplates, and the ethanol concentration in the batch cultures and fed-batch fermentations was measured. Tri-n-butyl phosphate [non-alcoholic solvent, density = 0.9727, solubility in water = 0.028% (w/v)] was used for solvent extraction when measuring the ethanol concentration from the yeast culture broth. The maximum detectable ethanol concentration was 8% (v/v) when 10 g potassium dichromate in 100 ml of 5 M sulfuric acid was used. The concentrations determined from the solvent extraction-dichromate oxidation methods were remarkably similar to those of gas chromatography in which samples were prepared from seven experiments, such as four batch cultures and three fed-batch fermentations.

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“…It was previously reported that the coculture system of S. cerevisiae and K. marxianus produced more ethanol from cheese whey powder solution compared to the monoculture system (25). Another study also reported that the coculture system of S. cerevisiae and K. marxianus was more effective than the monoculture system of K. marxianus for ethanol production from henequen juice and molasses (26).…”

Section: Table 1 Yields and End Products Of Batch Ethanol Fermentatimentioning

confidence: 94%

High-temperature ethanol fermentation by immobilized coculture of Kluyveromyces marxianus and Saccharomyces cerevisiae

Eiadpum¹,

Limtong²,

Phisalaphong³

2012

Journal of Bioscience and Bioengineering

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Ethanol production from henequen (Agave fourcroydes Lem.) juice and molasses by a mixture of two yeasts

Cited by 35 publications

References 15 publications

Two Bacillus Species Isolated from Rotting Wood Samples are Good Candidates for the Production of Bioethanol using Agave Biomass

Two Bacillus Species Isolated from Rotting Wood Samples are Good Candidates for the Production of Bioethanol using Agave Biomass

Measurement of ethanol concentration using solvent extraction and dichromate oxidation and its application to bioethanol production process

High-temperature ethanol fermentation by immobilized coculture of Kluyveromyces marxianus and Saccharomyces cerevisiae

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