Ethanol production from cellobiose by Zymobacter palmae carrying the Ruminocuccus albus β-glucosidase gene

Yanase, Hideshi; Yamamoto, Keiko; Sato, Dai; Okamoto, Kenji

doi:10.1016/j.jbiotec.2005.02.009

Cited by 19 publications

(7 citation statements)

References 21 publications

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“…Xylose and metabolites, such as lactate and acetate, in the culture broth were determined by HPLC as described (Yanase et al 2005b). Fermentation results are averages from three individual experiments.…”

Section: Fermentationmentioning

confidence: 99%

An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose

Wang

Chen

et al. 2007

Biotechnol Lett

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An ethanol-tolerant mutant, ET1, was isolated by an enrichment method from Escherichia coli JM109. Strains JM109 and ET1 were transformed with expression vector pZY507bc containing Zymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes, resulting in an ethanol-sensitive recombinant strain JMbc and an ethanol-tolerant recombinant strain, ET1bc. Alcohol dehydrogenase and pyruvate decarboxylase activities were 24 and 32% lower, respectively, in JMbc than in ET1bc. ET1bc fermented 10% (w/v) xylose to give 39.4 g ethanol/l (77%, theoretical yield), a 1.3-fold increase compared with the ethanol-sensitive strain JMbc.

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Section: Fermentationmentioning

confidence: 99%

An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose

Wang

Chen

et al. 2007

Biotechnol Lett

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“…This organism produces approximately 2 mol of ethanol per mol of glucose without accumulation of by-products and shows productivity similar to that of Z. mobilis (16). Moreover, we previously described a host-vector system for Z. palmae and showed that by using the system we could breed a strain capable of producing ethanol from cellobiose (22).…”

mentioning

confidence: 92%

“…But wild-type Z. palmae does not ferment xylose. We therefore investigated the possibility of conferring that ability on Z. palmae by introducing the E. coli genes encoding XI, XK, TA, and TK by using our previously described host-vector system (22). Here we report the simultaneous fermentation of xylose and glucose to ethanol by a recombinant Z. palmae strain expressing E. coli xylose catabolic enzymes.…”

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confidence: 99%

Genetic Engineering of Zymobacter palmae for Production of Ethanol from Xylose

Yanase

Sato

Yamamoto

et al. 2007

Appl Environ Microbiol

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Its metabolic characteristics suggest that Zymobacter palmae gen. nov., sp. nov. could serve as a useful new ethanol-fermenting bacterium, but its biotechnological exploitation will require certain genetic modifications. We therefore engineered Z. palmae so as to broaden the range of its fermentable sugar substrates to include the pentose sugar xylose. The Escherichia coli genes encoding the xylose catabolic enzymes xylose isomerase, xylulokinase, transaldolase, and transketolase were introduced into Z. palmae, where their expression was driven by the Zymomonas mobilis glyceraldehyde-3-phosphate dehydrogenase promoter. When cultured with 40 g/liter xylose, the recombinant Z. palmae strain was able to ferment 16.4 g/liter xylose within 5 days, producing 91% of the theoretical yield of ethanol with no accumulation of organic acids as metabolic by-products. Notably, xylose acclimation enhanced both the expression of xylose catabolic enzymes and the rate of xylose uptake into recombinant Z. palmae, which enabled the acclimated organism to completely and simultaneously ferment a mixture of 40 g/liter glucose and 40 g/liter xylose within 8 h, producing 95% of the theoretical yield of ethanol. Thus, efficient fermentation of a mixture of glucose and xylose to ethanol can be accomplished by using Z. palmae expressing E. coli xylose catabolic enzymes.

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“…Today, a number of microorganisms are known to have a natural or acquired ability to ferment cellobiose into ethanol. For example, S. cerevisiae , which accumulates 38 g/l of ethanol at 30°C (Choi et al 2022 ), Myceliophthora thermophila— 11.3 g/l of ethanol at 45–50°C, (Li et al 2020 ), Zymobacter palmae —10 g/l of ethanol at 30°C, (Yanase et al 2005 ). The O. polymorpha currently produces a maximum of only 5 g/l of ethanol at 45°C.…”

Section: Discussionmentioning

confidence: 99%

Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose

Vasylyshyn,

Dmytruk,

Sybirnyy

et al. 2024

FEMS Yeast Research

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Successful conversion of cellulosic biomass into biofuels requires organisms capable of efficiently utilizing xylose as well as cellodextrins and glucose. Ogataea (Hansenula) polymorpha is the natural xylose-metabolizing organism and is one of the most thermotolerant yeasts known, with a maximum growth temperature above 50°C. Cellobiose-fermenting strains, derivatives of an improved ethanol producer from xylose O. polymorpha BEP/cat8∆, were constructed in this work by the introduction of heterologous genes encoding cellodextrin transporters (CDTs) and intracellular enzymes (β-glucosidase or cellobiose phosphorylase) that hydrolyze cellobiose. For this purpose, the genes gh1-1 of β-glucosidase, CDT-1 m and CDT-2 m of cellodextrin transporters from Neurospora crassa and the CBP gene coding for cellobiose phosphorylase from Saccharophagus degradans, were successfully expressed in O. polymorpha. Through metabolic engineering and mutagenesis, strains BEP/cat8∆/gh1-1/CDT-1 m and BEP/cat8∆/CBP-1/CDT-2mAM were developed, showing improved parameters for high-temperature alcoholic fermentation of cellobiose. The study highlights the need for further optimization to enhance ethanol yields and elucidate cellobiose metabolism intricacies in O. polymorpha yeast. This is the first report of the successful development of stable methylotrophic thermotolerant strains of O. polymorpha capable of co-utilizing cellobiose, glucose, and xylose under high-temperature alcoholic fermentation conditions at 45°C.

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Ethanol production from cellobiose by Zymobacter palmae carrying the Ruminocuccus albus β-glucosidase gene

Cited by 19 publications

References 21 publications

An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose

An ethanol-tolerant recombinant Escherichia coli expressing Zymomonas mobilis pdc and adhB genes for enhanced ethanol production from xylose

Genetic Engineering of Zymobacter palmae for Production of Ethanol from Xylose

Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose

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