Ethanol Production and Other Bioproducts by Galactomyces geotrichum from Sugarcane Bagasse Hydrolysate

Lamounier, Kênia Francisca Resende; Rodrigues, Patrísia de Oliveira; Pasquini, Daniel; Santos, Alexandre Soares dos; Baffi, Milla Alves

doi:10.1007/s00284-019-01866-7

Cited by 12 publications

(8 citation statements)

References 28 publications

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“…Sugarcane bagasse was composed of 54.36% of cellulose, 13.52% of hemicelluloses and 26.14% of lignin (Table 1), indicating that the evaluated sample presents a lignocellulosic structure similar to those reported in previous studies (Rodrigues et al, 2017;Lamounier et al, 2020). This result showed SB as a rich source of polysaccharides for potential use in the biosynthesis of cellulases and hemicellulases.…”

Section: Chemical Composition Of the Substratessupporting

confidence: 77%

“…Among biofuels, cellulosic ethanol can be synthesized from the depolymerization of lignocellulose in fermentable sugars (Toquero and Bolado, 2014). In Brazil, the ethanol obtained from sugarcane bagasse is a promising strategy, since this by-product is generated in large quantities and does not compete with the food chain (Lamounier et al 2020). Hemicellulases and cellulases are lignocellulose-degrading enzymes responsible for the synergistic breakdown of biomass into free monosaccharides, with wide industrial application such as biofuels, pulp and paper industry, textile sector, detergents, feed, beverages, among others (Verma et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Production and characterization of cellulases and hemicellulases from a consortium between Pleurotus ostreatus and Aspergillus niger cultured in agro-industrial wastes

Martins

Gomes

Pasquini

et al. 2021

RSD

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The enzyme biosynthesis using agricultural wastes by solid state fermentation (SSF) and the study of their physicochemical properties are meaningful approaches to improve the biomass hydrolysis. Among them, β-glucosidases and β-xylosidases are key enzymes at the lignocellulose depolymerization, which act in the cleavage of oligosaccharides in monosaccharides. In this study, the production of hemicellulases and cellulases by Pleurotus ostreatus and Aspergillus niger monocultures or in consortium was investigated, using raw sugarcane bagasse (SB) and wheat bran (WB) as substrates. The highest enzymatic activities were observed in the crude extract produced by P. ostreatus PLO6 and A. niger SCBM4 consortium with 98.5, 62.9, 3.8, 12.4, 13.3 and 20.2 U/g for β-glucosidase (β-glu), β-xylosidase (Bxyl), filter paper cellulase (FPase), xylanase (Xyl), exoglucanase (Exgl) and endoglucanase (Engl), respectively. The pH and temperature effects on β-glu and β-xyl were characterized. Optimal activities were obtained at pH 4.0 and 45 °C for β-glu and 3.5 and 55 °C for β-xyl. Both enzymes were stable at acid pH and presented thermostability. The results indicated that the enzymatic cocktail demonstrated potential characteristics for future applications in saccharifications. The use of sugarcane bagasse and wheat bran for microbial growth contributed to aggregate value to these byproducts.

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Section: Chemical Composition Of the Substratessupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Production and characterization of cellulases and hemicellulases from a consortium between Pleurotus ostreatus and Aspergillus niger cultured in agro-industrial wastes

Martins

Gomes

Pasquini

et al. 2021

RSD

Self Cite

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“…The 10 mL of fermentation liquid was centrifuged (2500 × g ) for 10 min, and the supernatant was collected and diluted appropriately. Reducing sugar was measured using the method of Lamounier et al 35 …”

Section: Methodsmentioning

confidence: 99%

“…Ethanol content was measured according to the method of Ruz et al 34 Measurement of reducing sugar content in culture medium The 10 mL of fermentation liquid was centrifuged (2500 × g) for 10 min, and the supernatant was collected and diluted appropriately. Reducing sugar was measured using the method of Lamounier et al 35 Measurement of intracellular glycerol content For this, 10 mL of fermentation liquid was taken, centrifuged (2500 × g) for 10 min, then the supernatant was discarded, and 20 mL of 100 mmol L −1 Tris-HCl buffer (pH 7.0) was added for washing twice. The washed yeast was placed in a pre-cooled mortar and ground with liquid nitrogen to break the wall, and diluted with distilled water.…”

Section: Measurement Of Ethanol Content In Culture Mediummentioning

confidence: 99%

Effect of glucose levels on carbon flow rate, antioxidant status, and enzyme activity of yeast during fermentation

2022

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BACKGROUND:The physiological metabolism of yeast has a significant impact on the quality of fermentation products. The present study aimed to investigate yeast metabolism in response to a changing glucose content environment, especially in fermentation products, as well as the change of carbon flow rate, antioxidant status, and yeast enzyme activity. RESULTS: Yeast in a 0 g L −1 glucose level was subjected to carbon starvation stress, cell growth retardation and cell proliferation was significantly inadequate; in the logarithmic growth stage of yeast, at a 30 g L −1 glucose level, the carbon source mainly flowed to tricarboxylic acid cycle and pentose phosphate metabolism, cell division, proliferation, and increased cell growth. In later logarithmic growth period and stable period, carbon flowed into glycerol and trehalose metabolism, to cope with the environmental stress; yeast in 60 and 150 g L −1 glucose levels faced high glucose stress at the beginning, the content of reactive oxygen increased, malondialdehyde content increased, cell damage was reduced through the regulation of superoxide dismutase and catalase enzyme activities, and most of the carbon flowed into the metabolic pathway of ethanol, glycerol, and trehalose to cope with high glucose stress, the pentose phosphate pathway showed a large late influx, and NADPH also started to increase rapidly after 24 h. CONCLUSION: Yeast was stressed in a high-sugar environment and ensured the activity of yeast by preferentially increasing the metabolic intensity of trehalose, glycerol, and glycolytic metabolism, weakening tricarboxylic acid metabolism, and first weakening and then increasing pentose phosphate metabolism.

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“…Glycoside hydrolase activities on polysaccharides were assayed by incubating 0.1 mg/mL enzymes in an assay mixture containing 1% ( w / v ) of Avicel PH101, carboxymethyl cellulose (CMC), phosphoric acid-swollen cellulose, filter paper (waterman No. 1), oat spelt xylan, citrus peel pectin, or milled corn stover in MES buffer at 60 °C for 24 h. The released sugar concentration was estimated by the dinitrosalicylic acid (DNS) method using glucose as the standard [ 26 ] at time points of 0, 1, 2, 4, 8, and 24 h. Phosphoric acid-swollen cellulose was prepared as described previously [ 27 ]. Cellobiosidase activity was measured using cellobiose as the substrate in above reaction systems.…”

Section: Methodsmentioning

confidence: 99%

Synergy of Cellulase Systems between Acetivibrio thermocellus and Thermoclostridium stercorarium in Consolidated-Bioprocessing for Cellulosic Ethanol

et al. 2022

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Anaerobes harbor some of the most efficient biological machinery for cellulose degradation, especially thermophilic bacteria, such as Acetivibrio thermocellus and Thermoclostridium stercorarium, which play a fundamental role in transferring lignocellulose into ethanol through consolidated bioprocessing (CBP). In this study, we compared activities of two cellulase systems under varying kinds of hemicellulose and cellulose. A. thermocellus was identified to contribute specifically to cellulose hydrolysis, whereas T. stercorarium contributes to hemicellulose hydrolysis. The two systems were assayed in various combinations to assess their synergistic effects using cellulose and corn stover as the substrates. Their maximum synergy degrees on cellulose and corn stover were, respectively, 1.26 and 1.87 at the ratio of 3:2. Furthermore, co-culture of these anaerobes on the mixture of cellulose and xylan increased ethanol concentration from 21.0 to 40.4 mM with a high cellulose/xylan-to-ethanol conversion rate of up to 20.7%, while the conversion rates of T. stercorarium and A. thermocellus monocultures were 19.3% and 15.2%. The reason is that A. thermocellus had the ability to rapidly degrade cellulose while T. stercorarium co-utilized both pentose and hexose, the metabolites of cellulose degradation, to produce ethanol. The synergistic effect of cellulase systems and metabolic pathways in A. thermocellus and T. stercorarium provides a novel strategy for the design, selection, and optimization of ethanol production from cellulosic biomass through CBP.

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Ethanol Production and Other Bioproducts by Galactomyces geotrichum from Sugarcane Bagasse Hydrolysate

Cited by 12 publications

References 28 publications

Production and characterization of cellulases and hemicellulases from a consortium between Pleurotus ostreatus and Aspergillus niger cultured in agro-industrial wastes

Production and characterization of cellulases and hemicellulases from a consortium between Pleurotus ostreatus and Aspergillus niger cultured in agro-industrial wastes

Effect of glucose levels on carbon flow rate, antioxidant status, and enzyme activity of yeast during fermentation

Synergy of Cellulase Systems between Acetivibrio thermocellus and Thermoclostridium stercorarium in Consolidated-Bioprocessing for Cellulosic Ethanol

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