Ethanol may be used with the microdialysis technique to monitor blood flow changes in skeletal muscle: dialysate glucose concentration is blood‐flow‐dependent

Hickner, Robert C.; Rosdahl, Hans; Borg, Ingrid; Ungerstedt, Urban; Jorfeldt, L.; Henriksson, Jan

doi:10.1111/j.1748-1716.1991.tb09243.x

Cited by 95 publications

(85 citation statements)

References 2 publications

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“…In adipose tissue and skeletal muscle the ethanol ratio has been used to study local blood flow around the microdialysis probe (Hickner et al, 1991;Galitsky et al, 1993). A thorough methodological evaluation of this technique was made in vitro and in vivo in skeletal muscle, including a direct comparison with the radioactive xenon-washout technique (Hickner et al, 1992;.…”

Section: Resultsmentioning

confidence: 99%

Cholinoceptor‐mediated effects on glycerol output from human adipose tissue using in situ microdialysis

Andersson

Arner

1995

British J Pharmacology

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1 Possible cholinoceptor-mediated effects on lipolysis were investigated in vivo in human subcutaneous adipose tissue of non-obese, non-smoking, healthy subjects, by use of microdialysis. Cholinomimetic and sympathomimetic agents were added to the ingoing dialysate solvent. 2 Addition of nicotine to the perfusion solvent caused a concentration-dependent reversible increase in the levels of glycerol in the dialysate (lipolysis index). The opposite effect (also concentration-dependent and reversible) was caused by the addition of carbachol. The maximum effects were 100% stimulation and 50% inhibition, respectively, by nicotine and carbachol. Neither nicotine nor carbachol stimulated nutritive blood flow in adipose tissue (as measured with an ethanol escape technique). 3 The nicotine effect in situ was concentration-dependently counteracted by the nicotinic cholinoceptor antagonist, mecamylamine. Likewise, the carbachol effect was concentration-dependently counteracted by the muscarinic cholinoceptor antagonist, atropine. 4 When adipose tissue was pretreated with phentolamine plus propranolol in order to obtain a complete a and P-adrenoceptor blockade, the subsequent addition of nicotine or carbachol still induced an increase and decrease in dialysate glycerol levels (lipolytic or antilipolytic effects), respectively. When adipose tissue was pretreated with mecamylamine or atropine, the subsequent addition of acetylcholine caused a reversible decrease and increase, respectively, of the dialysate glycerol levels. 5 Nicotine and carbachol had no effects on glycerol release from human isolated subcutaneous fat cells that were incubated in vivo. 6 In conclusion, the data demonstrate a dual effect of the cholinoceptor system on glycerol output in human adipose tissue: stimulation through nicotinic receptors and inhibition through muscarinic receptors. These effects, which are not observed in vitro, are independent of the adrenergic system and the local blood flow and seem not to be mediated by a direct action on the fat cell.

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Section: Resultsmentioning

confidence: 99%

Cholinoceptor‐mediated effects on glycerol output from human adipose tissue using in situ microdialysis

Andersson

Arner

1995

British J Pharmacology

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“…36,38 Ethanol concentrations were measured both in the perfusate and the dialysate. The blood flow was estimated as ethanol clearance by dividing dialysate concentration by perfusate International Journal of Obesity Hormone sensitive lipase and weight loss M Kolehmainen et al concentration.…”

Section: Microdialysismentioning

confidence: 99%

Hormone sensitive lipase expression and adipose tissue metabolism show gender difference in obese subjects after weight loss

et al. 2002

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OBJECTIVE:The effect of weight reduction on hormone sensitive lipase (HSL) and lipoprotein lipase (LPL) gene expression and their relationship with adipose tissue metabolism were studied in massively obese men and women. SUBJECTS: Seventeen obese subjects (eight men, nine women) participated in the study (age 44 AE 2 y, weight 145 AE 8 kg, fat 40 AE 2% of body mass, mean AE s.e.m.), who were going through a gastric-banding operation for weight reduction. MEASUREMENTS: HSL and LPL mRNA expressions were analyzed using the reverse transcription competitive polymerase chain reaction. Subcutaneous fat lipolysis was measured in vivo by microdialysis and in vitro in isolated subcutaneous abdominal adipocytes. Measurements were done before and after 1 y of weight reduction. RESULTS: Significant reductions in weight (for men 7 20.3 AE 2.5%, for women 7 18.3 AE 2.1% (mean AE s.e.m.) and fat mass (for men 7 27.6 AE 7.9%, for women 7 21.8 AE 3.9%) were observed in both genders. In women HSL mRNA expression decreased by 31% (P ¼ 0.008) and LPL expression increased slightly, but nonsignificantly (42%, P ¼ 0.110). These changes were not observed in men. In men, inhibition of lipolysis with a 2 -adrenergic and adenosine agonist was improved (P ¼ 0.001) in isolated adipocytes. CONCLUSIONS: This study uncovers new differences between genders in adipocyte metabolism along with weight reduction. In women, the observed changes in HSL and LPL gene expression suggest that deposition of lipids into adipose tissue might be favored after weight reduction. In men, the results indicate improved responsiveness to inhibition in adipose tissue metabolism along with weight reduction.

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“…This issue was first raised when ethanol clearance from microdialysis perfusate was developed and validated [15] as a method of attaining a qualitative measure of local tissue blood flow at the site of microdialysis catheter insertion. Studies demonstrated that, in animals, gross changes in tissue blood flow alter the recovery of glucose by microdialysis [16] (i. e. an increase in local blood flow increases dialysate recovery of glucose) and similar findings have since been reported in human adipose and muscle tissues but with a less predictable effect of altered blood flow on the recovery of ECF lactate [17].…”

Section: Methodologic Considerationsmentioning

confidence: 61%

Microdialysis techniques in the study of brain and skeletal muscle

1997

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Ethanol may be used with the microdialysis technique to monitor blood flow changes in skeletal muscle: dialysate glucose concentration is blood‐flow‐dependent

Cited by 95 publications

References 2 publications

Cholinoceptor‐mediated effects on glycerol output from human adipose tissue using in situ microdialysis

Cholinoceptor‐mediated effects on glycerol output from human adipose tissue using in situ microdialysis

Hormone sensitive lipase expression and adipose tissue metabolism show gender difference in obese subjects after weight loss

Microdialysis techniques in the study of brain and skeletal muscle

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