Ethanol levels in honeybee hemolymph resulting from alcohol ingestion

Božič, Janko; DiCesare, John C.; Wells, Harrington; Abramson, Charles I.

doi:10.1016/j.alcohol.2007.04.003

Cited by 23 publications

(19 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, recent work has established a dose and time dependent response curve for the effects of ethanol on locomotor behavior in the honey bee, demonstrating that ethanol consumption has a profound effect on locomotion including reductions in walking behavior, increased grooming, and a loss of the righting reflex (Maze et al, 2006). Ethanol hemolymph (blood) levels also exhibit dose and time dependent changes after ethanol ingestion; the concentrations necessary to produce inebriation in the honey bee are similar to the levels observed in other invertebrate model systems and for mammals (Bozic, DiCesare, Wells, & Abranson, 2007; Maze et al, 2006). …”

Section: Introductionmentioning

confidence: 63%

Acute ethanol ingestion impairs appetitive olfactory learning and odor discrimination in the honey bee

Mustard

Edgar²,

Mazade

et al. 2008

Neurobiology of Learning and Memory

View full text Add to dashboard Cite

Invertebrates are valuable models for increasing our understanding of the effects of ethanol on the nervous system, but most studies on invertebrates and ethanol have focused on the effects of ethanol on locomotor behavior. In this work we investigate the influence of an acute dose of ethanol on appetitive olfactory learning in the honey bee (Apis mellifera), a model system for learning and memory. Adult worker honey bees were fed a range of doses (2.5, 5, 10 or 25%) of ethanol and then conditioned to associate an odor with a sucrose reward using either a simple or differential conditioning paradigm. Consumption of ethanol before conditioning significantly reduced both the rate of acquisition and the asymptotic strength of the association. Honey bees also exhibited a dose dependent reduction in arousal/attention during conditioning. Consumption of ethanol after conditioning did not affect recall 24 h later. The observed deficits in acquisition were not due to the affect of ethanol on gustatory sensitivity or motor function. However, honey bees given higher doses of ethanol had difficulty discriminating amongst different odors suggesting that ethanol consumption influences olfactory processing. Taken together, these results demonstrate that an acute dose of ethanol affects appetitive learning and olfactory perception in the honey bee.

show abstract

Section: Introductionmentioning

confidence: 63%

Acute ethanol ingestion impairs appetitive olfactory learning and odor discrimination in the honey bee

Mustard

Edgar²,

Mazade

et al. 2008

Neurobiology of Learning and Memory

View full text Add to dashboard Cite

show abstract

“…Bees are sensitive to ethanol concentrations comparable to those that affect mammals, nematodes, and fruit flies, but bees that consume ethanol display very slow kinetics of intoxication (Bozic, DiCesare, Wells, & Abramson, 2007). Hemolymph ethanol levels take up to 30 minutes to peak, and then they remain high for six to eight hours.…”

Section: Figure 231 Analysis Of Ethanol-induced Locomotor Behaviors Amentioning

confidence: 99%

The Genetics of Alcohol Responses of Invertebrate Model Systems

Rothenfluh

Troutwine

Ghezzi

et al. 2014

Neurobiology of Alcohol Dependence

View full text Add to dashboard Cite

“…Following intestinal dissection, the hemolymph of the dissected bees was collected according to the method of Bozic et al (2007). Briefly, the hemolymph of each bee was removed by making a small incision in the thorax and collected using a 10 lL pipette for a total of 2-3 lL per bee.…”

Section: Tissue Dissection and Hemolymph Collectionmentioning

confidence: 99%