Ethanol-Induced Perturbations to Planar Lipid Bilayer Structures

Setiawan, Iwan; Blanchard, G. J.

doi:10.1021/jp410305m

Cited by 22 publications

(40 citation statements)

References 58 publications

(120 reference statements)

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“…Recently, Setiawan and Blanchard showed that ethanol interferes with the size and distribution of cholesterol domains in planar lipid bilayer structures [ 37 ]. To determine whether plasma membrane cholesterol could be involved in the inhibitory effect of ethanol, we evaluated the effect of Mβ alone or together with ethanol on FcεRI-mediated degranulation.…”

Section: Resultsmentioning

confidence: 99%

Ethanol Inhibits High-Affinity Immunoglobulin E Receptor (FcεRI) Signaling in Mast Cells by Suppressing the Function of FcεRI-Cholesterol Signalosome

et al. 2015

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Ethanol has multiple effects on biochemical events in a variety of cell types, including the high-affinity immunoglobulin E receptor (FcεRI) signaling in antigen-activated mast cells. However, the underlying molecular mechanism remains unknown. To get better understanding of the effect of ethanol on FcεRI-mediated signaling we examined the effect of short-term treatment with non-toxic concentrations of ethanol on FcεRI signaling events in mouse bone marrow-derived mast cells. We found that 15 min exposure to ethanol inhibited antigen-induced degranulation, calcium mobilization, expression of proinflammatory cytokine genes (tumor necrosis factor-α, interleukin-6, and interleukin-13), and formation of reactive oxygen species in a dose-dependent manner. Removal of cellular cholesterol with methyl-β-cyclodextrin had a similar effect and potentiated some of the inhibitory effects of ethanol. In contrast, exposure of the cells to cholesterol-saturated methyl-β-cyclodextrin abolished in part the inhibitory effect of ethanol on calcium response and production of reactive oxygen species, supporting lipid-centric theories of ethanol action on the earliest stages of mast cell signaling. Further studies showed that exposure to ethanol and/or removal of cholesterol inhibited early FcεRI activation events, including tyrosine phosphorylation of the FcεRI β and γ subunits, SYK kinases, LAT adaptor protein, phospholipase Cγ, STAT5, and AKT and internalization of aggregated FcεRI. Interestingly, ethanol alone, and particularly in combination with methyl-β-cyclodextrin, enhanced phosphorylation of negative regulatory tyrosine 507 of LYN kinase. Finally, we found that ethanol reduced passive cutaneous anaphylactic reaction in mice, suggesting that ethanol also inhibits FcεRI signaling under in vivo conditions. The combined data indicate that ethanol interferes with early antigen-induced signaling events in mast cells by suppressing the function of FcεRI-cholesterol signalosomes at the plasma membrane.

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Section: Resultsmentioning

confidence: 99%

Ethanol Inhibits High-Affinity Immunoglobulin E Receptor (FcεRI) Signaling in Mast Cells by Suppressing the Function of FcεRI-Cholesterol Signalosome

et al. 2015

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“…The instrument used to acquire fluorescence anisotropy decay images has been described previously. [36] Briefly, the instrument is based on an inverted microscope optical configuration (Nikon Eclipse Ti−U). All images were collected using a 10x microscope objective.…”

Section: Methodsmentioning

confidence: 99%

Diffusional motion as a gauge of fluidity and interfacial adhesion. Supported alkylphosphonate monolayers

Baumler

Reidy

Blanchard

2016

Journal of Colloid and Interface Science

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We report on the use of diffusion measurements to gauge the fluidity and surface binding properties of a molecular monolayer. The monolayer film consists of octadecyl-1-phosphonic acid (ODPA) and controlled amounts of a lyso-phosphatidic acid tagged with the fluorescent probe BODIPY (BLPA). The monolayer films were formed using a Langmuir-Blodgett (LB) trough and deposited onto a glass slide. Monolayer morphology was characterized during film formation using Brewster angle microscopy (BAM). Fluorescence Recovery After Photobleaching (FRAP) microscopy was used to measure translational diffusion of BLPA and Fluorescence Anisotropy Decay Imaging (FADI) was used to measure rotational diffusion of the BLPA chromophore. These results provide information on the motional freedom of the probe and, importantly, on the strength of interaction between the probe and the support. Compositional variations in the monolayer give rise to changes in constituent dynamics that reflect intermolecular interactions.

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“…DOPC and DOPE are representatives of two bulk lipids found across practically all eukaryotes [43][44][45][46], with PE also being common in prokaryotes [37,47]. The phase behavior of DOPE and DOPC has been investigated in considerable detail [39,40,42,48,49], making deviations from the typical behavior of these systems generally straightforward to identify [29,38,50,51]. Therefore, they are useful for characterizing the behavior of lipids in response to minor additives [29,36,38].…”

Section: Resultsmentioning

confidence: 99%

Bioactive Metabolites of Marine Origin Have Unusual Effects on Model Membrane Systems

et al. 2020

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Marine sponges and soft corals have yielded novel compounds with antineoplastic and antimicrobial activities. Their mechanisms of action are poorly understood, and in most cases, little relevant experimental evidence is available on this topic. In the present study, we investigated whether agelasine D (compound 1) and three agelasine analogs (compound 2–4) as well as malonganenone J (compound 5), affect the physical properties of a simple lipid model system, consisting of dioleoylphospahtidylcholine and dioleoylphosphatidylethanolamine. The data indicated that all the tested compounds increased stored curvature elastic stress, and therefore, tend to deform the bilayer which occurs without a reduction in the packing stress of the hexagonal phase. Furthermore, lower concentrations (1%) appear to have a more pronounced effect than higher ones (5–10%). For compounds 4 and 5, this effect is also reflected in phospholipid headgroup mobility assessed using 31P chemical shift anisotropy (CSA) values of the lamellar phases. Among the compounds tested, compound 4 stands out with respect to its effects on the membrane model systems, which matches its efficacy against a broad spectrum of pathogens. Future work that aims to increase the pharmacological usefulness of these compounds could benefit from taking into account the compound effects on the fluid lamellar phase at low concentrations.

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Ethanol-Induced Perturbations to Planar Lipid Bilayer Structures

Cited by 22 publications

References 58 publications

Ethanol Inhibits High-Affinity Immunoglobulin E Receptor (FcεRI) Signaling in Mast Cells by Suppressing the Function of FcεRI-Cholesterol Signalosome

Ethanol Inhibits High-Affinity Immunoglobulin E Receptor (FcεRI) Signaling in Mast Cells by Suppressing the Function of FcεRI-Cholesterol Signalosome

Diffusional motion as a gauge of fluidity and interfacial adhesion. Supported alkylphosphonate monolayers

Bioactive Metabolites of Marine Origin Have Unusual Effects on Model Membrane Systems

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