Ethanol induced NF-κB activation protects against cell injury in cultured rat gastric mucosal epithelium

Abstract

Mustonen H, Hietaranta A, Puolakkainen P, Kemppainen E, Paimela H, Kiviluoto T, Kivilaakso E. Ethanol induced NF-B activation protects against cell injury in cultured rat gastric mucosal epithelium.

A Novel Transcription Mechanism Activated by Ethanol

“…Indeed, ethanol has been reported to regulate the activity of many transcription factors (17, 18, 36 -39). For example, ethanol has been shown to reduce or increase the binding activity of NF-B to an NF-B oligonucleotide probe in rat pancreatic acinar cells (36) and gastric mucosal epithelia, respectively (37). In addition, it has been suggested that ethanol increases the activity of transcription factors and STAT3, specificity protein-1 (Sp1), and AP1, thereby up-regulating IL-10 expression in human monocytes (38).…”

Taurocholate Potentiates Ethanol-Induced NF-κB Activation and Inhibits Caspase-3 Activity in Cultured Rat Gastric Mucosal Cells

“…Positive controls were obtained by transfecting the cells with Lamin A/C siRNA (100 nM, D-001050-01-05, Dharmacon, Lafayette,CO) and following the Lamin A/C expression in these cells. The effect of relA siRNA transfections on relA protein expression is given in [2].…”

“…We have recently shown that exposure to modest concentrations of ethanol (EtOH) induces transcription factor nuclear factor-jB (NF-jB) activation in gastric surface epithelial cells. Inhibition of this activation led to increased apoptotic activity, suggesting that NF-jB defends the epithelium against EtOH insult [1,2]. It is not known whether other ulcerogenic agents, such as bile acids or acetylsalicylic acid (ASA), exert a similar protective action in gastric surface epithelial cells.…”

“…TC, one of the bile acids, has been used in a model of experimental acute pancreatitis by injecting it into the pancreatic duct, which leads to NF-jB activation. This regulation is probably indirect activation mediated by cytokine production [5] rather than a direct mechanism as shown with EtOH in gastric mucosa [1,2]. TC is known to cause changes in cell membrane making it more permeable to other extracellular agents [6], increasing apical cell membrane permeability [7], and acting as a detergent eventually disrupting the cell membrane [8].…”

Influência da exposição à fumaça do cigarro e ao etanol sobre as alterações da mucosa do esôfago induzidas por dieta modificada em camundongos C57BL/6

“…Study in vitro has shown that ethanol exposure induced moderately Cox-2 expression in the rat gastric mucosal cell line cells (Mustonen et al, 2007). Here, we not observed difference of the ciclooxygenase-2 expression between the ethanol treated-groups and the respective control groups.…”