Ethanol-induced neutrophil apoptosis is mediated through nitric oxide

Singhal, Pravin C.; Patel, Puja; Nahar, N; Franki, Nicholas; Kapasi, Aditi A.; Reddy, Krishna; Shah, Neena; Nwakoby, Izuchukwu E.; Mehrotra, Bhoomi

doi:10.1002/jlb.66.6.930

Cited by 30 publications

(18 citation statements)

References 46 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neutrophil functions such as chemotaxis and production of reactive oxygen species have previously shown to be affected by ethanol and polyphenols (Patel et al, 1996;Lu et al, 2001). Acute ETOH exposure in humans (five glasses of wine or more) induced neutrophil apoptosis (Singhal et al, 1999a), which is expected to impair neutrophil functions. In the present study, however, no significant effects on neutrophil as well as monocyte phagocytosis were observed with the different beverages suggesting that neither ETOH nor red grape-associated polyphenols were effective.…”

Section: Discussionmentioning

confidence: 99%

Daily moderate amounts of red wine or alcohol have no effect on the immune system of healthy men

et al. 2003

View full text Add to dashboard Cite

Objective: To investigate whether the daily intake of red wine (RW) at a dose which inversely correlates with cardiovascular disease (CVD) risk modulates immune functions in healthy men. Design: Randomized single-blind trial with four intervention periods. Setting: The Institute of Nutritional Physiology, Federal Research Centre for Nutrition, Karlsruhe, Germany. Subjects: A total of 24 healthy males with moderate alcohol consumption patterns were recruited and all completed the study. Intervention: Participants consumed 500 ml of RW (12% ethanol (ETOH)) or 500 ml of a 12% ETOH dilution per day for a period of 2 weeks. To control the potential effects of RW polyphenols, accordingly 500 ml/day of dealcoholized red wine (DRW) and of red grape juice (RGJ) were given. The following immune parameters were measured before beverage consumption and at 1 and 2 weeks following beverage consumption: phagocytic activity of neutrophils and monocytes, production of tumor necrosis factor-alpha (TNFa), interleukin-2 and -4, transforming growth factor-b, TNFa mRNA, lymphocyte proliferation, lytic activity of natural killer cells, and percentage of apoptotic lymphocytes. Results: Consumption of a moderate volume of alcohol with RW and with a 12% ETOH dilution had no effect on immune functions in healthy males. Consumption of polyphenol-rich beverages (DRW and RGJ) did not affect immunity-related parameters. Conclusions: Daily moderate consumption of alcohol and of RW for 2 weeks at doses which inversely correlate with CVD risk has no adverse effects on human immune cell functions. Polyphenol-rich beverages such as RGJ and DRW further do not suppress immune responses in healthy men.

show abstract

Section: Discussionmentioning

confidence: 99%

Daily moderate amounts of red wine or alcohol have no effect on the immune system of healthy men

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Ten milliliters of peripheral blood was collected from each donor in heparinized tubes. Neutrophils were separated by a standard technique of density-gradient centrifugation over Ficoll-Hypaque (29).…”

Section: Methodsmentioning

confidence: 99%

“…It has been demonstrated that interleukin-2, gamma interferon, tumor necrosis factor (TNF) alpha, granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor, and glucocorticoids increase the life span of neutrophils (4-6, 14, 36). On the contrary, nitric oxide generation, both endogenous and exogenous, may accelerate neutrophil apoptosis (8,29).…”

mentioning

confidence: 99%

Fas-Mediated Apoptosis of Neutrophils in Sera of Patients with Infection

et al. 2001

Self Cite

View full text Add to dashboard Cite

In the presence of infection, neutropenia is considered to be a marker of poor prognosis; conversely, neutrophilia may not be a determinant of a better prognosis. Since apoptotic neutrophils are compromised functionally, we evaluated the effect of infection on neutrophil apoptosis. The rate of apoptosis was greater for neutrophils isolated from patients with infection than for healthy controls. Escherichia coli did not directly modulate the rate of neutrophil apoptosis. However, sera from infected patients promoted (P < 0.001) neutrophil apoptosis. Interestingly, the sera of patients with different types of infection (gram negative, gram positive, or culture negative) exerted a more or less identical response on neutrophil apoptosis. Sera of infected patients showed a fivefold greater content of FasL compared to controls. Moreover, anti-FasL antibody partly attenuated the infected-serum-induced neutrophil apoptosis. In in vitro studies, E. coli enhanced monocyte FasL expression. Moreover, conditioned media prepared from activated macrophages from control mice showed enhanced apoptosis of human as well as mouse neutrophils. On the contrary, conditioned media prepared from activated macrophages isolated from FasL-deficient mice induced only a mild degree of neutrophil apoptosis. These results suggest that neutrophils in patients with infection undergo apoptosis at an accelerated rate. Infection not only promoted monocyte expression of FasL but also increased FasL content of the serum. Because the functional status of apoptotic cells is compromised, a significant number of neutrophils may not be participating in the body's defense. Since neutrophils play the most important role in innate immunity, their compromised status in the presence of infection may transfer the host defense burden from an innate response to acquired immunity. The present study provides some insight into the lack of correlation between neutrophilia and the outcome of infection.

show abstract

“…43 Whether this cell death comprises apoptosis or merely necrosis, however, remains unclear. An induction of neutrophil apoptosis has also been observed using both traditional NO donors such as SNP 44 and different sources of NO such as GSNO (0.5-5 mM). 45 Blaylock et al 46 reported that GEA 3162 (10 mM) increased neutrophil apoptosis as assessed by annexin V binding to exposed phosphatidylserine (PS) at 4 and 8 h, but not at 16 h. On the other hand, the ONOO À donor, SIN-1 (1 mM), showed no significant increase in PS exposure compared to control cells, although there was a small enhancement of annexin V binding at 4 h. The effects of these two compounds were also studied by Ward et al, 10 who demonstrated that concentrations of 10-100 mM GEA 3162 and 0.3-3 mM SIN-1 enhanced caspase-dependent morphological neutrophil apoptosis at 6 and 20 h, and 30-100 mM GEA 3162 increased annexin V binding at 6 h. In contrast to Blaylock's study, however, the increase in morphological apoptosis by 10 mM GEA 3162 was not significant at 6 h, whereas Blaylock observed significant differences at just 4 h. The principal methodological difference between the two studies is in the culture conditions.…”

Section: Neutrophilsmentioning

confidence: 96%

Nitric oxide: a key regulator of myeloid inflammatory cell apoptosis

et al. 2003

View full text Add to dashboard Cite

Apoptosis of inflammatory cells is a critical event in the resolution of inflammation, as failure to undergo this form of cell death leads to increased tissue damage and exacerbation of the inflammatory response. Many factors are able to influence the rate of apoptosis in neutrophils, eosinophils, monocytes and macrophages. Among these is the signalling molecule nitric oxide (NO), which possesses both anti-and proapoptotic properties, depending on the concentration and flux of NO, and also the source from which NO is derived. This review summarises the differential effects of NO on inflammatory cell apoptosis and outlines potential mechanisms that have been proposed to explain such actions.

show abstract

Ethanol-induced neutrophil apoptosis is mediated through nitric oxide

Cited by 30 publications

References 46 publications

Daily moderate amounts of red wine or alcohol have no effect on the immune system of healthy men

Daily moderate amounts of red wine or alcohol have no effect on the immune system of healthy men

Fas-Mediated Apoptosis of Neutrophils in Sera of Patients with Infection

Nitric oxide: a key regulator of myeloid inflammatory cell apoptosis

Contact Info

Product

Resources

About